Most viewed
Editor-in-Chief Melissa Norton, MD
Biology Editor Penelope Webb, PhD
In-house Editor J. Ann Le Good
BMC Biotechnology: Most viewed articles in past year [more info ]
Figures given indicate the abstract, full text and PDF accesses on BioMed Central for each article, over the last year. All research articles are also available via the PubMed Central archive, so the total number of accesses to each article is significantly higher. Lists of
most-viewed articles over the last 30 days and of
all time most-viewed articles are also available
most viewed articles over the last year from all BioMed Central Journals
1. Accesses 8105
Methodology article
Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions Frederique Ponchel, Carmel Toomes, Kieran Bransfield, Fong T Leong, Susan H Douglas, Sarah L Field, Sandra M Bell, Valerie Combaret, Alain Puisieux, Alan J Mighell, Philip A Robinson, Chris F Inglehearn, John D Isaacs, Alex F Markham BMC Biotechnology 2003, 3 :18 (13 October 2003) [Abstract ] [Full text ] [PDF ] [PubMed ] [Related articles ] [Cited on BioMed Central ]
2. Accesses 4866
Research article
Isolation of mesenchymal stem cells from equine umbilical cord blood Thomas G Koch, Tammy Heerkens, Preben D Thomsen, Dean H Betts BMC Biotechnology 2007, 7 :26 (30 May 2007) [Abstract ] [Full text ] [PDF ] [PubMed ] [Related articles ] [Cited on BioMed Central ]
3. Accesses 4698
Methodology article
Use of polyethyleneimine polymer in cell culture as attachment factor and lipofection enhancer Ajith R Vancha, Suman Govindaraju, Kishore VL Parsa, Madhuri Jasti, Maribel Gonz lez-Garc a, Rafael P Ballestero BMC Biotechnology 2004, 4 :23 (15 October 2004) [Abstract ] [Full text ] [PDF ] [PubMed ] [Related articles ]
4. Accesses 4130
Research article
Quantum dot imaging for embryonic stem cells Shuan Lin, Xiaoyan Xie, Manishkumar R Patel, Yao-Hung Yang, Zongjin Li, Feng Cao, Oliver Gheysens, Yan Zhang, Sanjiv S Gambhir, Jiang Hong Rao, Joseph C Wu BMC Biotechnology 2007, 7 :67 (9 October 2007) [Abstract ] [Full text ] [PDF ] [PubMed ] [Related articles ] [Cited on BioMed Central ]
5. Accesses 3799
Methodology article
Performance of high-throughput DNA quantification methods Kashif A Haque, Ruth M Pfeiffer, Michael B Beerman, Jeff P Struewing, Stephen J Chanock, Andrew W Bergen BMC Biotechnology 2003, 3 :20 (28 October 2003) [Abstract ] [Full text ] [PDF ] [PubMed ] [Related articles ] [Cited on BioMed Central ]
6. Accesses 3657
Methodology article
A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods Justin C Yarrow, Zachary E Perlman, Nicholas J Westwood, Timothy J Mitchison BMC Biotechnology 2004, 4 :21 (9 September 2004) [Abstract ] [Full text ] [PDF ] [PubMed ] [Related articles ] [F1000 Biology ] [Cited on BioMed Central ]
7. Accesses 3518
Methodology article
Design and cloning strategies for constructing shRNA expression vectors Glen J McIntyre, Gregory C Fanning BMC Biotechnology 2006, 6 :1 (5 January 2006) [Abstract ] [Full text ] [PDF ] [PubMed ] [Related articles ]
8. Accesses 3403
Research article
Single chain Fab (scFab) fragment Michael Hust, Thomas Jostock, Christian Menzel, Bernd Voedisch, Anja Mohr, Mariam Brenneis, Martina I Kirsch, Doris Meier, Stefan D bel BMC Biotechnology 2007, 7 :14 (8 March 2007) [Abstract ] [Full text ] [PDF ] [PubMed ] [Related articles ] [Cited on BioMed Central ]
9. Accesses 3122
Methodology article
p HUSH: a single vector system for conditional gene expressionDaniel C Gray, Klaus P Hoeflich, Li Peng, Zhenyu Gu, Alvin Gogineni, Lesley J Murray, Mike Eby, Noelyn Kljavin, Somasekar Seshagiri, Mary J Cole, David P Davis BMC Biotechnology 2007, 7 :61 (26 September 2007) [Abstract ] [Full text ] [PDF ] [PubMed ] [Related articles ]
10. Accesses 2791
Methodology article
Rapid and reliable extraction of genomic DNA from various wild-type and transgenic plants Tae-Jin Kang, Moon-Sik Yang BMC Biotechnology 2004, 4 :20 (2 September 2004) [Abstract ] [Full text ] [PDF ] [PubMed ] [Related articles ] [Cited on BioMed Central ]