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	76. 2381 Accesses	Methodology article Highly efficient concentration of lenti- and retroviral vector preparations by membrane adsorbers and ultrafiltration Katrin Zimmermann, Oliver Scheibe, Andreas Kocourek, Jutta Muelich, Elke Jurkiewicz, Alexander Pfeifer BMC Biotechnology 2011, 11:55 (20 May 2011) Abstract \| Full text \| PDF \| PubMed
	77. 2379 Accesses	Research article Efficient Production of an Engineered Apoptin from Chicken Anemia Virus in a Recombinant E. coli for Tumor Therapeutic Applications Meng-Shiou Lee, Fang-Chun Sun, Chi-Hung Huang, Yi-Yang Lien, Shin-Huei Feng, Guan-Hua Lai, Meng-Shiunn Lee, Jung Chao, Hsi-Jien Chen, Jason T C Tzen, Hao-Yuan Cheng BMC Biotechnology 2012, 12:27 (6 June 2012) Abstract \| Full text \| PDF \| PubMed
	78. 2319 Accesses	Methodology article mRNA transcript quantification in archival samples using multiplexed, color-coded probes Patricia P Reis, Levi Waldron, Rashmi S Goswami, Wei Xu, Yali Xuan, Bayardo Perez-Ordonez, Patrick Gullane, Jonathan Irish, Igor Jurisica, Suzanne Kamel-Reid BMC Biotechnology 2011, 11:46 (9 May 2011) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary The NanoString nCounter™ gene expression system, a novel technology based on multiplexed, color-coded probes, performs better than quantitative real-time PCR to accurately quantify mRNA transcripts in archived formalin-fixed, paraffin-embedded oral carcinoma samples.
	79. 2316 Accesses	Methodology article Construction of siRNA/miRNA expression vectors based on a one-step PCR process Jun Xu, Jie Zeng, Gang Wan, Gui Hu, Hong Yan, Li Ma BMC Biotechnology 2009, 9:53 (2 June 2009) Abstract \| Full text \| PDF \| PubMed
	80. 2295 Accesses	Research article Prevention of post-surgical abdominal adhesions by a novel biodegradable thermosensitive PECE hydrogel. Bing Yang, ChangYang Gong, ZhiYong Qian, Xia Zhao, ZhengYu Li, XiaoRong Qi, ShengTao Zhou, Qian Zhong, Feng Luo, YuQuan Wei BMC Biotechnology 2010, 10:65 (9 September 2010) Abstract \| Full text \| PDF \| PubMed
	81. 2283 Accesses	Research article pEPito: a significantly improved non-viral episomal expression vector for mammalian cells Rudolf Haase, Orestis Argyros, Suet-Ping Wong, Richard P Harbottle, Hans J Lipps, Manfred Ogris, Terese Magnusson, Maria Pinto, Jürgen Haas, Armin Baiker BMC Biotechnology 2010, 10:20 (15 March 2010) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary pEPito, a novel non-viral protein expression vector is significantly smaller, contains only one transcription unit and 60% less CpG motives in comparison to the prototype pEPI-1 vector, considerably improving efficacy both in vitro and in vivo.
	82. 2277 Accesses	Methodology article A new approach to 'megaprimer' polymerase chain reaction mutagenesis without an intermediate gel purification step Rajiv Tyagi, Richard Lai, Ronald G Duggleby BMC Biotechnology 2004, 4:2 (26 February 2004) Abstract \| Full text \| PDF \| PubMed
	83. 2271 Accesses	Methodology article PCR-based gene synthesis to produce recombinant proteins for crystallization Damien Marsic, Ronny C Hughes, Miranda L Byrne-Steele, Joseph D Ng BMC Biotechnology 2008, 8:44 (29 April 2008) Abstract \| Full text \| PDF \| PubMed
	84. 2271 Accesses	Research article Hydrocarbon phenotyping of algal species using pyrolysis-gas chromatography mass spectrometry Dinesh K Barupal, Tobias Kind, Shankar L Kothari, Do Lee, Oliver Fiehn BMC Biotechnology 2010, 10:40 (21 May 2010) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Hydrocarbon phenotyping of algae can be achieved cost-effectively using substructure annotation algorithms to assess data from pyrolysis-gas chromatography quadrupole mass spectrometry, enabling rapid discrimination between algal strains for biofuel applications.
	85. 2249 Accesses	Research article A 28 nt long synthetic 5^′UTR (synJ) as an enhancer of transgene expression in dicotyledonous plants Shaveta Kanoria, Pradeep Kumar Burma BMC Biotechnology 2012, 12:85 (10 November 2012) Abstract \| Full text \| PDF \| PubMed
	86. 2243 Accesses	Review Less is more: strategies to remove marker genes from transgenic plants Yuan-Yeu Yau, C Neal Stewart BMC Biotechnology 2013, 13:36 (23 April 2013) Abstract \| Provisional PDF \| PubMed \| Editor’s summary Yuan-Yeu Yau and C Neal Stewart review existing strategies to remove selectable marker genes from transgenic plants, including site specific recombination systems, and explore emerging technologies for increased precision genome modification.
	87. 2184 Accesses	Research article Construction and model-based analysis of a promoter library for E. coli: an indispensable tool for metabolic engineering Marjan De Mey, Jo Maertens, Gaspard J Lequeux, Wim K Soetaert, Erick J Vandamme BMC Biotechnology 2007, 7:34 (18 June 2007) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	88. 2172 Accesses	Research article Optimization of culture conditions to produce high yields of active Acetobacter sp. CCTCC M209061 cells for anti-Prelog reduction of prochiral ketones Xiao-Hong Chen, Wen-Yong Lou, Min-Hua Zong, Thomas J Smith BMC Biotechnology 2011, 11:110 (20 November 2011) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	89. 2170 Accesses	Methodology article A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering Morten HH Nørholm BMC Biotechnology 2010, 10:21 (16 March 2010) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	90. 2169 Accesses	Methodology article Improved Tet-responsive promoters with minimized background expression Rainer Loew, Niels Heinz, Mathias Hampf, Hermann Bujard, Manfred Gossen BMC Biotechnology 2010, 10:81 (24 November 2010) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	91. 2150 Accesses	Research article Thrombolytic effects of Douchi Fibrinolytic enzyme from Bacillus subtilis LD-8547 in vitro and in vivo Jun Yuan, Jing Yang, Zhenhong Zhuang, Yanling Yang, Ling Lin, Shihua Wang BMC Biotechnology 2012, 12:36 (2 July 2012) Abstract \| Full text \| PDF \| PubMed
	92. 2135 Accesses	Research article Effect of different freezing rates during cryopreservation of rat mesenchymal stem cells using combinations of hydroxyethyl starch and dimethylsulfoxide Yahaira Naaldijk, Marek Staude, Viktoriya Fedorova, Alexandra Stolzing BMC Biotechnology 2012, 12:49 (13 August 2012) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	93. 2125 Accesses	Commentary Armed and accurate: engineering cytotoxic T cells for eradication of leukemia Marko Radic BMC Biotechnology 2012, 12:6 (8 February 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Marko Radic discusses recent advances in cancer immunotherapy, whereby cytotoxic T cells from patients with leukemia are turned into efficient and specific killers of their own cancer cells.
	94. 2125 Accesses	Methodology article High-efficiency protein expression in plants from agroinfection-compatible Tobacco mosaic virus expression vectors John A Lindbo BMC Biotechnology 2007, 7:52 (27 August 2007) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	95. 2115 Accesses	Methodology article Assaying proline hydroxylation in recombinant collagen variants by liquid chromatography-mass spectrometry S W Chan, John Greaves, Nancy A Da Silva, Szu-Wen Wang BMC Biotechnology 2012, 12:51 (17 August 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary A novel liquid chromatography-mass spectrometry method evaluates proline hydroxylation in recombinant collagen, allowing accurate determination of hydroxylation in yeast expression systems and represents a useful alternative to conventional amino acid analysis.
	96. 2105 Accesses	Research article Identification of immunogenic proteins and generation of antibodies against Salmonella Typhimurium using phage display Torsten Meyer, Thomas Schirrmann, André Frenzel, Sebastian Miethe, Janin Stratmann-Selke, Gerald F Gerlach, Katrin Strutzberg-Minder, Stefan Dübel, Michael Hust BMC Biotechnology 2012, 12:29 (15 June 2012) Abstract \| Full text \| PDF \| PubMed
	97. 2103 Accesses	Research article Design and development of a peptide-based adiponectin receptor agonist for cancer treatment Laszlo Otvos, Eva Haspinger, Francesca La Russa, Federica Maspero, Patrizia Graziano, Ilona Kovalszky, Sandor Lovas, Kaushik Nama, Ralf Hoffmann, Daniel Knappe, Marco Cassone, John Wade, Eva Surmacz BMC Biotechnology 2011, 11:90 (5 October 2011) Abstract \| Full text \| PDF \| PubMed
	98. 2088 Accesses	Research article Validation of glypican-3-specific scFv isolated from paired display/secretory yeast display library Yonghai Li, Donald L Siegel, Nathalie Scholler, David E Kaplan BMC Biotechnology 2012, 12:23 (7 May 2012) Abstract \| Full text \| PDF \| PubMed
	99. 2083 Accesses	Research article Exobiopolymer production of Ophiocordyceps dipterigena BCC 2073: optimization, production in bioreactor and characterization Kanokarn Kocharin, Pranee Rachathewee, Jean-Jacques Sanglier, Wai Prathumpai BMC Biotechnology 2010, 10:51 (12 July 2010) Abstract \| Full text \| PDF \| PubMed
	100. 2071 Accesses	Research article New biotechnological perspectives of a NADH oxidase variant from Thermus thermophilus HB27 as NAD⁺-recycling enzyme Javier Rocha-Martín, Daniel Vega, Juan M Bolivar, Cesar A Godoy, Aurelio Hidalgo, José Berenguer, José M Guisán, Fernando López-Gallego BMC Biotechnology 2011, 11:101 (3 November 2011) Abstract \| Full text \| PDF \| PubMed \| 1 comment