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LRE qPCR Website (Bob Rutledge, 20 November 2014)

Please note that a website has been constructed that provides additional information about LRE qPCR, in addition to providing the most current version of the LRE Analyzer, a fully featured Java desktop program that automates LRE-based quantification. The site also includes a three part video that provides a detailed overview of how LRE qPCR was developed and tested. read full comment

Comment on: Rutledge et al. BMC Biotechnology, 8:47

New email contact (Dafne Solera PhD, 15 October 2013)

The corrisponding author new email address: read full comment

Comment on: Liu et al. BMC Biotechnology, 12:28

Further clarification (Dafne Solera PhD, 20 September 2013)

The authors would like to further clarify that they have retracted this article [1] due to the ethical misconduct of Karel Bezouska. His misappropriation of data fundamentally changes the main findings of the article. Specifically, the upper amino acid sequence in Figure 1 (by KB) in BMC Biotechnol 11:2 was incorrect, therefore Nit-ANigWT (nitrilase purified from Aspergillus niger K10) and Nit-ANigRec (nitrilase expressed in E. coli harbouring a nit gene from A. niger K10) were not variants of the same enzyme as hypothesized in the original article. The different biochemical properties of Nit-ANigWT and Nit-ANigRec discussed in the paper were caused by a significant difference in the primary structures of these enzymes [2].... read full comment

Comment on: Kaplan et al. BMC Biotechnology, 13:57

Details of preparing the solubilized extract for RNeasy column isolation of the RNA (Elizabeth Floyd, 24 October 2012)

The solubilized bone extract is separated from the bone material by centrifugation at 8,600 x g for 15 seconds at room temperature as described in the manuscript. The solubilized extract is then transferred to a new microtube and the TriReagent is removed from the solubilized extract by adding chloroform at a TriReagent:chloroform ratio of 5:1. So we add 0.2 ml chloroform to the extract and mix by inverting the tube a few times. The phases are allowed to begin separating at room temperature x 4-5 minutes before centrifuging at 12,000 x g for 15 minutes at 4C. The resulting supernatant** (aqueous phase) is transferred to a new microfuge tube and 1 volume of 70% ethanol is added to the extract before placing the extract-ethanol mixture onto the RNeasy... read full comment

Comment on: Carter et al. BMC Biotechnology, 12:5

Abstract correction (Fernando Lopez Gallego, 19 December 2011)

In the abstract, the sentence " to asymmetric reduction of prochiral substrates in order to obtain enantiopure compounds." is wrong and we meant "to enantionselective oxidation of racemic substrates in order to obtain enatiopure compounds." read full comment

Comment on: Rocha-Martín et al. BMC Biotechnology, 11:101

Correction (Hilko van der Voet, 25 March 2011)

Unfortunately, an error ocurred during the final phase of publishing.

In the section Methods, subsection Linear mixed models, there is a numbered list summarizing the appropriate calculations for performing the difference and equivalence tests.
Under numbers 2 and 4 in this list, the first argument of the lsd function in the equations for the confidence limits should be GR instead of GC.
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Comment on: van der Voet et al. BMC Biotechnology, 11:15

current adress (mickael malnoy, 16 December 2010)

Mickael MALNOY PhD
Research and Innovation Center
Structural and Functional Genomics
Fondazione Edmund Mach (FEM)
Istituto Agrario San Michele (IASMA)
Via Mach 1,
38010 San Michele all'Adige (TN)

Phone office: +39 0461 61 55 36 begin_of_the_skype_highlighting              +39 0461 61 55 36      end_of_the_skype_highlighting
Phone lab: +39 0461 61 54 16
Fax: +39 0461 65 09 56

E-mail: read full comment

Comment on: Borejsza-Wysocka et al. BMC Biotechnology, 10:41

Figure 1 (matthias braun, 01 October 2010)

The pattern of green fluorescence in Fig. 1C looks quite different from Fig. 1A: the fluorescence is punctate and does not appear cytosolic as in 1A. Have both images been obtained under the same conditions, e.g. with similar laser power? Can it be excluded that Fig. 1C shows autofluorescence following strong excitation? read full comment

Comment on: Lefebvre et al. BMC Biotechnology, 10:28

Updated version for the Java program (Bob Rutledge, 15 October 2009)

The prototypic Java program provided as supplementary materials has been updated, which fixes a number of bugs and provides a somewhat improved algorithm for LRE window selection. A copy of this updated program can be obtained by request to the corresponding author.

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Comment on: Rutledge et al. BMC Biotechnology, 8:47

Are Antibodies better than Antibiotics? (Marko Radic, 23 September 2009)

The overuse of antibiotics is contributing to problems of drug resistance and outbreaks of exotic parasites in poultry. Alternatives are sorely needed. Here is an approach using recombinant antibodies expressed in feed plants to passively provide "immunity" to birds. Will there be an outcry over using recombinant DNA that is one step removed from the human consumer? Are dangers (perceived and real) associated with GMF also applicable to the consumption of chickens that feed on a genetically-modified plant? Presumably, a breast or drumstick of such a bird would not contain any traces of the antibody proteins. Still a concern? read full comment

Comment on: Zimmermann et al. BMC Biotechnology, 9:79

Equation 2 is incorrect (Bob Rutledge, 23 October 2008)

Equation 2 should read:E = 10-Slope - 1It is also important to note that it is essential to fix the fluorescence threshold (Ft) to a single value across all runs, in order to avoid artificial quantitative variances.The mathematics of standard curve construction and evaluation of quantitative variance generated by standard curves are addressed in detail in an earlier study:Rutledge RG, Côté C: Mathematics of quantitative kinetic PCR and the application of standard curves.Nucleic Acids Res 2003, 31(16):e93. read full comment

Comment on: Roberts et al. BMC Biotechnology, 8:57