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		Research article Immobilized Rhizopus oryzae lipase catalyzed synthesis of palm stearin and cetyl alcohol wax esters: Optimization by Response Surface Methodology Mohamed Sellami, Imen Aissa, Fakher Frikha, Youssef Gargouri, Nabil Miled BMC Biotechnology 2011, 11:68 (17 June 2011) Abstract \| Full text \| PDF \| PubMed
		Methodology article RNA interference in marine and freshwater sponges: actin knockdown in Tethya wilhelma and Ephydatia muelleri by ingested dsRNA expressing bacteria Ajna S Rivera, Jörg U Hammel, Karri M Haen, Elizabeth S Danka, Brandon Cieniewicz, Ian P Winters, Dora Posfai, Gert Wörheide, Dennis V Lavrov, Scott W Knight, Malcolm S Hill, April L Hill, Michael Nickel BMC Biotechnology 2011, 11:67 (16 June 2011) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Soaking developing sponges in a solution containing double-stranded RNA (dsRNA), or feeding them bacteria expressing dsRNA, can lead to gene knock-down by RNA interference in both marine and freshwater sponges allowing loss-of-function manipulation in these emerging model organisms.
		Methodology article Nano-biolistics: a method of biolistic transfection of cells and tissues using a gene gun with novel nanometer-sized projectiles John A O'Brien, Sarah CR Lummis BMC Biotechnology 2011, 11:66 (10 June 2011) Abstract \| Full text \| PDF \| PubMed
		Research article A novel subtilase with NaCl-activated and oxidant-stable activity from Virgibacillus sp. SK37 Ekkarat Phrommao, Jirawat Yongsawatdigul, Sureelak Rodtong, Montarop Yamabhai BMC Biotechnology 2011, 11:65 (9 June 2011) Abstract \| Full text \| PDF \| PubMed
		Research article Modification of the loops in the ligand-binding site turns avidin into a steroid-binding protein Tiina A Riihimäki, Soili Hiltunen, Martina Rangl, Henri R Nordlund, Juha AE Määttä, Andreas Ebner, Peter Hinterdorfer, Markku S Kulomaa, Kristiina Takkinen, Vesa P Hytönen BMC Biotechnology 2011, 11:64 (9 June 2011) Abstract \| Full text \| PDF \| PubMed
		Research article Facile, high efficiency immobilization of lipase enzyme on magnetic iron oxide nanoparticles via a biomimetic coating Yuhong Ren, Jose G Rivera, Lihong He, Harsha Kulkarni, Dong-Keun Lee, Phillip B Messersmith BMC Biotechnology 2011, 11:63 (8 June 2011) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Lipase can be immobilized efficiently under aqueous conditions by coating magnetic nanoparticles with a polydopamine biomimetic inspired by mussel adhesive proteins, with the enzyme still retaining high activity even after many cycles of magnetic separation and reuse.
		Research article Evaluation of biolistic gene transfer methods in vivo using non-invasive bioluminescent imaging techniques Jixiang Xia, Angela Martinez, Henry Daniell, Steven N Ebert BMC Biotechnology 2011, 11:62 (2 June 2011) Abstract \| Full text \| PDF \| PubMed
		Research article Antibodies on demand: a fast method for the production of human scFvs with minimal amounts of antigen Ingrid Babel, Rodrigo Barderas, Alberto Peláez-García, J Ignacio Casal BMC Biotechnology 2011, 11:61 (2 June 2011) Abstract \| Full text \| PDF \| PubMed
		Research article Purification of functional baculovirus particles from silkworm larval hemolymph and their use as nanoparticles for the detection of human prorenin receptor (PRR) binding Tatsuya Kato, Fumiaki Suzuki, Enoch Y Park BMC Biotechnology 2011, 11:60 (2 June 2011) Abstract \| Full text \| PDF \| PubMed
		Research article Purification of phage display-modified bacteriophage T4 by affinity chromatography Anna Oślizło, Paulina Miernikiewicz, Agnieszka Piotrowicz, Barbara Owczarek, Agnieszka Kopciuch, Grzegorz Figura, Krystyna Dąbrowska BMC Biotechnology 2011, 11:59 (31 May 2011) Abstract \| Full text \| PDF \| PubMed
		Research article Construction of uricase-overproducing strains of Hansenula polymorpha and its application as biological recognition element in microbial urate biosensor Kostyantyn V Dmytruk, Oleh V Smutok, Olena V Dmytruk, Wolfgang Schuhmann, Andriy A Sibirny BMC Biotechnology 2011, 11:58 (25 May 2011) Abstract \| Full text \| PDF \| PubMed
		Methodology article Cell-free synthesis of a functional G protein-coupled receptor complexed with nanometer scale bilayer discs Jian-Ping Yang, Tatiana Cirico, Federico Katzen, Todd C Peterson, Wieslaw Kudlicki BMC Biotechnology 2011, 11:57 (23 May 2011) Abstract \| Full text \| PDF \| PubMed
		Research article A visible, targeted high-efficiency gene delivery and transfection strategy Qiao-Ying Yuan, Jing Huang, Bao-Cheng Chu, Xing-Sheng Li, Liang-Yi Si BMC Biotechnology 2011, 11:56 (21 May 2011) Abstract \| Full text \| PDF \| PubMed
		Methodology article Highly efficient concentration of lenti- and retroviral vector preparations by membrane adsorbers and ultrafiltration Katrin Zimmermann, Oliver Scheibe, Andreas Kocourek, Jutta Muelich, Elke Jurkiewicz, Alexander Pfeifer BMC Biotechnology 2011, 11:55 (20 May 2011) Abstract \| Full text \| PDF \| PubMed
		Methodology article Isolation and analysis of high quality nuclear DNA with reduced organellar DNA for plant genome sequencing and resequencing Kerry A Lutz, Wenqin Wang, Anna Zdepski, Todd P Michael BMC Biotechnology 2011, 11:54 (20 May 2011) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
		Research article Construction of high quality Gateway™ entry libraries and their application to yeast two-hybrid for the monocot model plant Brachypodium distachyon Shuanghe Cao, Chamindika L Siriwardana, Roderick W Kumimoto, Ben F Holt BMC Biotechnology 2011, 11:53 (19 May 2011) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
		Research article Determination of some significant batch culture conditions affecting acetyl-xylan esterase production by Penicillium notatum NRRL-1249 S Atta, S Ali, MN Akhtar, I Haq BMC Biotechnology 2011, 11:52 (16 May 2011) Abstract \| Full text \| PDF \| PubMed
		Research article Identification of a novel temperature sensitive promoter in cho cells Haruthai Thaisuchat, Martina Baumann, Jens Pontiller, Friedemann Hesse, Wolfgang Ernst BMC Biotechnology 2011, 11:51 (12 May 2011) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
		Methodology article Multiple strategies to improve sensitivity, speed and robustness of isothermal nucleic acid amplification for rapid pathogen detection Yanhong Tong, Bertrand Lemieux, Huimin Kong BMC Biotechnology 2011, 11:50 (11 May 2011) Abstract \| Full text \| PDF \| PubMed
		Research article Potential errors in conventional DOT measurement techniques in shake flasks and verification using a rotating flexitube optical sensor Sven Hansen, Frank Kensy, Andreas Käser, Jochen Büchs BMC Biotechnology 2011, 11:49 (11 May 2011) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
		Correction Sequencing bias: comparison of different protocols of MicroRNA library construction Geng Tian, ShangQuang Gan, XuYang Yin, Hong Luo, XiaoHong Xu, Lars Bolund, XiuQing Zhang, Ning Li BMC Biotechnology 2011, 11:48 (11 May 2011) Abstract \| Full text \| PDF \| PubMed
		Research article Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris Kristina Nordén, Maria Agemark, Jonas ÅH Danielson, Erik Alexandersson, Per Kjellbom, Urban Johanson BMC Biotechnology 2011, 11:47 (10 May 2011) Abstract \| Full text \| PDF \| PubMed
		Methodology article mRNA transcript quantification in archival samples using multiplexed, color-coded probes Patricia P Reis, Levi Waldron, Rashmi S Goswami, Wei Xu, Yali Xuan, Bayardo Perez-Ordonez, Patrick Gullane, Jonathan Irish, Igor Jurisica, Suzanne Kamel-Reid BMC Biotechnology 2011, 11:46 (9 May 2011) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary The NanoString nCounter™ gene expression system, a novel technology based on multiplexed, color-coded probes, performs better than quantitative real-time PCR to accurately quantify mRNA transcripts in archived formalin-fixed, paraffin-embedded oral carcinoma samples.
		Research article Efficient production of human acidic fibroblast growth factor in pea (Pisum sativum L.) plants by agroinfection of germinated seeds Yajun Fan, Wei Li, Junjie Wang, Jingying Liu, Meiying Yang, Duo Xu, Xiaojuan Zhu, Xingzhi Wang BMC Biotechnology 2011, 11:45 (6 May 2011) Abstract \| Full text \| PDF \| PubMed
		Methodology article Estimation of affinities of ligands in mixtures via magnetic recovery of target-ligand complexes and chromatographic analyses: chemometrics and an experimental model Xiaolan Yang, Yanling Xie, Jun Pu, Hua Zhao, Juan Liao, Yonghua Yuan, Sha Zhu, Gaobo Long, Chun Zhang, Huidong Yuan, Yiwen Chen, Fei Liao BMC Biotechnology 2011, 11:44 (5 May 2011) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central

	All
		Research article Immobilized Rhizopus oryzae lipase catalyzed synthesis of palm stearin and cetyl alcohol wax esters: Optimization by Response Surface Methodology Mohamed Sellami, Imen Aissa, Fakher Frikha, Youssef Gargouri, Nabil Miled BMC Biotechnology 2011, 11:68 (17 June 2011) Abstract \| Full text \| PDF \| PubMed
		Methodology article RNA interference in marine and freshwater sponges: actin knockdown in Tethya wilhelma and Ephydatia muelleri by ingested dsRNA expressing bacteria Ajna S Rivera, Jörg U Hammel, Karri M Haen, Elizabeth S Danka, Brandon Cieniewicz, Ian P Winters, Dora Posfai, Gert Wörheide, Dennis V Lavrov, Scott W Knight, Malcolm S Hill, April L Hill, Michael Nickel BMC Biotechnology 2011, 11:67 (16 June 2011) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Soaking developing sponges in a solution containing double-stranded RNA (dsRNA), or feeding them bacteria expressing dsRNA, can lead to gene knock-down by RNA interference in both marine and freshwater sponges allowing loss-of-function manipulation in these emerging model organisms.
		Methodology article Nano-biolistics: a method of biolistic transfection of cells and tissues using a gene gun with novel nanometer-sized projectiles John A O'Brien, Sarah CR Lummis BMC Biotechnology 2011, 11:66 (10 June 2011) Abstract \| Full text \| PDF \| PubMed
		Research article A novel subtilase with NaCl-activated and oxidant-stable activity from Virgibacillus sp. SK37 Ekkarat Phrommao, Jirawat Yongsawatdigul, Sureelak Rodtong, Montarop Yamabhai BMC Biotechnology 2011, 11:65 (9 June 2011) Abstract \| Full text \| PDF \| PubMed
		Research article Modification of the loops in the ligand-binding site turns avidin into a steroid-binding protein Tiina A Riihimäki, Soili Hiltunen, Martina Rangl, Henri R Nordlund, Juha AE Määttä, Andreas Ebner, Peter Hinterdorfer, Markku S Kulomaa, Kristiina Takkinen, Vesa P Hytönen BMC Biotechnology 2011, 11:64 (9 June 2011) Abstract \| Full text \| PDF \| PubMed
		Research article Facile, high efficiency immobilization of lipase enzyme on magnetic iron oxide nanoparticles via a biomimetic coating Yuhong Ren, Jose G Rivera, Lihong He, Harsha Kulkarni, Dong-Keun Lee, Phillip B Messersmith BMC Biotechnology 2011, 11:63 (8 June 2011) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Lipase can be immobilized efficiently under aqueous conditions by coating magnetic nanoparticles with a polydopamine biomimetic inspired by mussel adhesive proteins, with the enzyme still retaining high activity even after many cycles of magnetic separation and reuse.
		Research article Evaluation of biolistic gene transfer methods in vivo using non-invasive bioluminescent imaging techniques Jixiang Xia, Angela Martinez, Henry Daniell, Steven N Ebert BMC Biotechnology 2011, 11:62 (2 June 2011) Abstract \| Full text \| PDF \| PubMed
		Research article Antibodies on demand: a fast method for the production of human scFvs with minimal amounts of antigen Ingrid Babel, Rodrigo Barderas, Alberto Peláez-García, J Ignacio Casal BMC Biotechnology 2011, 11:61 (2 June 2011) Abstract \| Full text \| PDF \| PubMed
		Research article Purification of functional baculovirus particles from silkworm larval hemolymph and their use as nanoparticles for the detection of human prorenin receptor (PRR) binding Tatsuya Kato, Fumiaki Suzuki, Enoch Y Park BMC Biotechnology 2011, 11:60 (2 June 2011) Abstract \| Full text \| PDF \| PubMed
		Research article Purification of phage display-modified bacteriophage T4 by affinity chromatography Anna Oślizło, Paulina Miernikiewicz, Agnieszka Piotrowicz, Barbara Owczarek, Agnieszka Kopciuch, Grzegorz Figura, Krystyna Dąbrowska BMC Biotechnology 2011, 11:59 (31 May 2011) Abstract \| Full text \| PDF \| PubMed
		Research article Construction of uricase-overproducing strains of Hansenula polymorpha and its application as biological recognition element in microbial urate biosensor Kostyantyn V Dmytruk, Oleh V Smutok, Olena V Dmytruk, Wolfgang Schuhmann, Andriy A Sibirny BMC Biotechnology 2011, 11:58 (25 May 2011) Abstract \| Full text \| PDF \| PubMed
		Methodology article Cell-free synthesis of a functional G protein-coupled receptor complexed with nanometer scale bilayer discs Jian-Ping Yang, Tatiana Cirico, Federico Katzen, Todd C Peterson, Wieslaw Kudlicki BMC Biotechnology 2011, 11:57 (23 May 2011) Abstract \| Full text \| PDF \| PubMed
		Research article A visible, targeted high-efficiency gene delivery and transfection strategy Qiao-Ying Yuan, Jing Huang, Bao-Cheng Chu, Xing-Sheng Li, Liang-Yi Si BMC Biotechnology 2011, 11:56 (21 May 2011) Abstract \| Full text \| PDF \| PubMed
		Methodology article Highly efficient concentration of lenti- and retroviral vector preparations by membrane adsorbers and ultrafiltration Katrin Zimmermann, Oliver Scheibe, Andreas Kocourek, Jutta Muelich, Elke Jurkiewicz, Alexander Pfeifer BMC Biotechnology 2011, 11:55 (20 May 2011) Abstract \| Full text \| PDF \| PubMed
		Methodology article Isolation and analysis of high quality nuclear DNA with reduced organellar DNA for plant genome sequencing and resequencing Kerry A Lutz, Wenqin Wang, Anna Zdepski, Todd P Michael BMC Biotechnology 2011, 11:54 (20 May 2011) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
		Research article Construction of high quality Gateway™ entry libraries and their application to yeast two-hybrid for the monocot model plant Brachypodium distachyon Shuanghe Cao, Chamindika L Siriwardana, Roderick W Kumimoto, Ben F Holt BMC Biotechnology 2011, 11:53 (19 May 2011) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
		Research article Determination of some significant batch culture conditions affecting acetyl-xylan esterase production by Penicillium notatum NRRL-1249 S Atta, S Ali, MN Akhtar, I Haq BMC Biotechnology 2011, 11:52 (16 May 2011) Abstract \| Full text \| PDF \| PubMed
		Research article Identification of a novel temperature sensitive promoter in cho cells Haruthai Thaisuchat, Martina Baumann, Jens Pontiller, Friedemann Hesse, Wolfgang Ernst BMC Biotechnology 2011, 11:51 (12 May 2011) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
		Methodology article Multiple strategies to improve sensitivity, speed and robustness of isothermal nucleic acid amplification for rapid pathogen detection Yanhong Tong, Bertrand Lemieux, Huimin Kong BMC Biotechnology 2011, 11:50 (11 May 2011) Abstract \| Full text \| PDF \| PubMed
		Research article Potential errors in conventional DOT measurement techniques in shake flasks and verification using a rotating flexitube optical sensor Sven Hansen, Frank Kensy, Andreas Käser, Jochen Büchs BMC Biotechnology 2011, 11:49 (11 May 2011) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
		Correction Sequencing bias: comparison of different protocols of MicroRNA library construction Geng Tian, ShangQuang Gan, XuYang Yin, Hong Luo, XiaoHong Xu, Lars Bolund, XiuQing Zhang, Ning Li BMC Biotechnology 2011, 11:48 (11 May 2011) Abstract \| Full text \| PDF \| PubMed
		Research article Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris Kristina Nordén, Maria Agemark, Jonas ÅH Danielson, Erik Alexandersson, Per Kjellbom, Urban Johanson BMC Biotechnology 2011, 11:47 (10 May 2011) Abstract \| Full text \| PDF \| PubMed
		Methodology article mRNA transcript quantification in archival samples using multiplexed, color-coded probes Patricia P Reis, Levi Waldron, Rashmi S Goswami, Wei Xu, Yali Xuan, Bayardo Perez-Ordonez, Patrick Gullane, Jonathan Irish, Igor Jurisica, Suzanne Kamel-Reid BMC Biotechnology 2011, 11:46 (9 May 2011) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary The NanoString nCounter™ gene expression system, a novel technology based on multiplexed, color-coded probes, performs better than quantitative real-time PCR to accurately quantify mRNA transcripts in archived formalin-fixed, paraffin-embedded oral carcinoma samples.
		Research article Efficient production of human acidic fibroblast growth factor in pea (Pisum sativum L.) plants by agroinfection of germinated seeds Yajun Fan, Wei Li, Junjie Wang, Jingying Liu, Meiying Yang, Duo Xu, Xiaojuan Zhu, Xingzhi Wang BMC Biotechnology 2011, 11:45 (6 May 2011) Abstract \| Full text \| PDF \| PubMed
		Methodology article Estimation of affinities of ligands in mixtures via magnetic recovery of target-ligand complexes and chromatographic analyses: chemometrics and an experimental model Xiaolan Yang, Yanling Xie, Jun Pu, Hua Zhao, Juan Liao, Yonghua Yuan, Sha Zhu, Gaobo Long, Chun Zhang, Huidong Yuan, Yiwen Chen, Fei Liao BMC Biotechnology 2011, 11:44 (5 May 2011) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central