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		Methodology article Pattern analysis approach reveals restriction enzyme cutting abnormalities and other cDNA library construction artifacts using raw EST data Sun Zhou, Guoli Ji, Xiaolin Liu, Pei Li, James Moler, John E Karro, Chun Liang BMC Biotechnology 2012, 12:16 (3 May 2012) Abstract \| Full text \| PDF \| PubMed
		Research article GMO detection using a bioluminescent real time reporter (BART) of loop mediated isothermal amplification (LAMP) suitable for field use Guy Kiddle, Patrick Hardinge, Neil Buttigieg, Olga Gandelman, Clint Pereira, Cathal J McElgunn, Manuela Rizzoli, Rebecca Jackson, Nigel Appleton, Cathy Moore, Laurence C Tisi, James AH Murray BMC Biotechnology 2012, 12:15 (30 April 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Genetically modified maize DNA can be effectively detected even at low levels of contamination by the coupling of loop mediated isothermal amplification (LAMP) to a bioluminescent output produced in real-time (BART).
		Research article Setting up a platform for plant-based influenza virus vaccine production in South Africa Elizabeth Mortimer, James M Maclean, Sandiswa Mbewana, Amelia Buys, Anna-Lise Williamson, Inga I Hitzeroth, Edward P Rybicki BMC Biotechnology 2012, 12:14 (26 April 2012) Abstract \| Full text \| PDF \| PubMed
		Correspondence Comments on the paper "A statistical assessment of differences and equivalences between genetically modified and reference plant varieties" by van der Voet et al. 2011 Keith J Ward, Margaret A Nemeth, Cavell Brownie, Bonnie Hong, Rod A Herman, Regina Oberdoerfer BMC Biotechnology 2012, 12:13 (25 April 2012) Abstract \| Full text \| PDF \| PubMed
		Correction Correction: BTI-Tnao38, a new cell line derived from Trichoplusia ni, is permissive for AcMNPV infection and produces high levels of recombinant proteins Yoshi Hashimoto, Sheng Zhang, Shiying Zhang, Yun-Ru Chen, Gary W Blissard BMC Biotechnology 2012, 12:12 (24 April 2012) Abstract \| Full text \| PDF \| PubMed
		Methodology article Dual-lifetime referencing (DLR): a powerful method for on-line measurement of internal pH in carrier-bound immobilized biocatalysts Caterina Boniello, Torsten Mayr, Juan M Bolivar, Bernd Nidetzky BMC Biotechnology 2012, 12:11 (28 March 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary A Dual-Lifetime Referencing (DLR) method based on the luminophores fluorescein and Ru(dpp) allows the measurement of pH within the particles of carrier-bound immobilized enzymes and has potential as an analytical tool in biocatalysis.
		Methodology article A novel PCR-based method for high throughput prokaryotic expression of antimicrobial peptide genes Tao Ke, Su Liang, Jin Huang, Han Mao, Jibao Chen, Caihua Dong, Junyan Huang, Shengyi Liu, Jianxiong Kang, Dongqi Liu, Xiangdong Ma BMC Biotechnology 2012, 12:10 (23 March 2012) Abstract \| Full text \| PDF \| PubMed
		Research article The Bxb1 recombination system demonstrates heritable transmission of site-specific excision in Arabidopsis James G Thomson, Ronald Chan, Jamison Smith, Roger Thilmony, Yuan-Yeu Yau, YueJu Wang, David W Ow BMC Biotechnology 2012, 12:9 (21 March 2012) Abstract \| Full text \| PDF \| PubMed
		Research article A designer hyper interleukin 11 (H11) is a biologically active cytokine Hanna Dams-Kozlowska, Katarzyna Gryska, Eliza Kwiatkowska-Borowczyk, Dariusz Izycki, Stefan Rose-John, Andrzej Mackiewicz BMC Biotechnology 2012, 12:8 (21 March 2012) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
		Research article M cell targeting by a Claudin 4 targeting peptide can enhance mucosal IgA responses David D Lo, Jun Ling, A Holly Eckelhoefer BMC Biotechnology 2012, 12:7 (13 March 2012) Abstract \| Full text \| PDF \| PubMed
		Commentary Armed and accurate: engineering cytotoxic T cells for eradication of leukemia Marko Radic BMC Biotechnology 2012, 12:6 (8 February 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Marko Radic discusses recent advances in cancer immunotherapy, whereby cytotoxic T cells from patients with leukemia are turned into efficient and specific killers of their own cancer cells.
		Methodology article An improved method for isolation of RNA from bone Lauren E Carter, Gail Kilroy, Jeffrey M Gimble, Z Elizabeth Floyd BMC Biotechnology 2012, 12:5 (19 January 2012) Abstract \| Full text \| PDF \| PubMed \| 1 comment
		Research article Comparison and transfer testing of multiplex ligation detection methods for GM plants Gabriella Ujhelyi, Jeroen P van Dijk, Theo W Prins, Marleen M Voorhuijzen, AM Angeline Van Hoef, Henriek G Beenen, Dany Morisset, Kristina Gruden, Esther J Kok BMC Biotechnology 2012, 12:4 (19 January 2012) Abstract \| Full text \| PDF \| PubMed
		Methodology article Creation and validation of a ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells Asmita Patel, Anisleidys Muñoz, Katherine Halvorsen, Priyamvada Rai BMC Biotechnology 2012, 12:3 (16 January 2012) Abstract \| Full text \| PDF \| PubMed
		Research article Construction and transformation of a Thermotoga-E. coli shuttle vector Dongmei Han, Stephen M Norris, Zhaohui Xu BMC Biotechnology 2012, 12:2 (6 January 2012) Abstract \| Full text \| PDF \| PubMed
		Methodology article Single/low-copy integration of transgenes in Caenorhabditis elegans using an ultraviolet trimethylpsoralen method Eriko Kage-Nakadai, Hiroyuki Kobuna, Osamu Funatsu, Muneyoshi Otori, Keiko Gengyo-Ando, Sawako Yoshina, Sayaka Hori, Shohei Mitani BMC Biotechnology 2012, 12:1 (5 January 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary A method utilizing ultraviolet trimethylpsoralen (UV/TMP) based on familiar extrachromosomal transgenics offers an alternative to Mos1 transposon or microparticle bombardment in the creation of single- or low-copy chromosomal integrated lines of Caenorhabditis elegans.
		Research article Antibody degradation in tobacco plants: a predominantly apoplastic process Verena K Hehle, Matthew J Paul, Pascal M Drake, Julian KC Ma, Craig J van Dolleweerd BMC Biotechnology 2011, 11:128 (30 December 2011) Abstract \| Full text \| PDF \| PubMed
		Research article Incorporation of albumin fusion proteins into fibrin clots in vitro and in vivo: comparison of different fusion motifs recognized by factor XIIIa William P Sheffield, Louise J Eltringham-Smith BMC Biotechnology 2011, 11:127 (20 December 2011) Abstract \| Full text \| PDF \| PubMed
		Research article In situ gastrointestinal protection against anthrax edema toxin by single-chain antibody fragment producing lactobacilli Kasper Andersen, Harold Marcotte, Beatriz Álvarez, Prosper N Boyaka, Lennart Hammarström BMC Biotechnology 2011, 11:126 (20 December 2011) Abstract \| Full text \| PDF \| PubMed
		Research article Transmission electron microscopy characterization of fluorescently labelled amyloid β 1-40 and α-synuclein aggregates Valerie L Anderson, Watt W Webb BMC Biotechnology 2011, 11:125 (19 December 2011) Abstract \| Full text \| PDF \| PubMed
		Methodology article Real-time PCR-based assay to quantify the relative amount of human and mouse tissue present in tumor xenografts Sergio Y Alcoser, David J Kimmel, Suzanne D Borgel, John P Carter, Kelly M Dougherty, Melinda G Hollingshead BMC Biotechnology 2011, 11:124 (16 December 2011) Abstract \| Full text \| PDF \| PubMed
		Research article Adsorption characteristics of an enteric virus-binding protein to norovirus, rotavirus and poliovirus Takahiro Imai, Daisuke Sano, Takayuki Miura, Satoshi Okabe, Keishi Wada, Yoshifumi Masago, Tatsuo Omura BMC Biotechnology 2011, 11:123 (16 December 2011) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary A broad spectrum virus-binding protein derived from the bacterial chaperon protein GroEL and able to bind rotavirus, norovirus and poliovirus, could be used to detect diverse enteric viruses simultaneously in water and fecal samples.
		Methodology article Targeted sequencing library preparation by genomic DNA circularization Samuel Myllykangas, Georges Natsoulis, John M Bell, Hanlee P Ji BMC Biotechnology 2011, 11:122 (14 December 2011) Abstract \| Full text \| PDF \| PubMed
		Methodology article Combining M-FISH and Quantum Dot technology for fast chromosomal assignment of transgenic insertions Mohammed Yusuf, David LV Bauer, Daniel M Lipinski, Robert E MacLaren, Richard Wade-Martins, Kalim U Mir, Emanuela V Volpi BMC Biotechnology 2011, 11:121 (13 December 2011) Abstract \| Full text \| PDF \| PubMed
		Methodology article GAL1-SceI directed site-specific genomic (gsSSG) mutagenesis: a method for precisely targeting point mutations in S. cerevisiae Sarah Piccirillo, Hsiao-Lin Wang, Thomas J Fisher, Saul M Honigberg BMC Biotechnology 2011, 11:120 (5 December 2011) Abstract \| Full text \| PDF \| PubMed

	All
		Methodology article Pattern analysis approach reveals restriction enzyme cutting abnormalities and other cDNA library construction artifacts using raw EST data Sun Zhou, Guoli Ji, Xiaolin Liu, Pei Li, James Moler, John E Karro, Chun Liang BMC Biotechnology 2012, 12:16 (3 May 2012) Abstract \| Full text \| PDF \| PubMed
		Research article GMO detection using a bioluminescent real time reporter (BART) of loop mediated isothermal amplification (LAMP) suitable for field use Guy Kiddle, Patrick Hardinge, Neil Buttigieg, Olga Gandelman, Clint Pereira, Cathal J McElgunn, Manuela Rizzoli, Rebecca Jackson, Nigel Appleton, Cathy Moore, Laurence C Tisi, James AH Murray BMC Biotechnology 2012, 12:15 (30 April 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Genetically modified maize DNA can be effectively detected even at low levels of contamination by the coupling of loop mediated isothermal amplification (LAMP) to a bioluminescent output produced in real-time (BART).
		Research article Setting up a platform for plant-based influenza virus vaccine production in South Africa Elizabeth Mortimer, James M Maclean, Sandiswa Mbewana, Amelia Buys, Anna-Lise Williamson, Inga I Hitzeroth, Edward P Rybicki BMC Biotechnology 2012, 12:14 (26 April 2012) Abstract \| Full text \| PDF \| PubMed
		Correspondence Comments on the paper "A statistical assessment of differences and equivalences between genetically modified and reference plant varieties" by van der Voet et al. 2011 Keith J Ward, Margaret A Nemeth, Cavell Brownie, Bonnie Hong, Rod A Herman, Regina Oberdoerfer BMC Biotechnology 2012, 12:13 (25 April 2012) Abstract \| Full text \| PDF \| PubMed
		Correction Correction: BTI-Tnao38, a new cell line derived from Trichoplusia ni, is permissive for AcMNPV infection and produces high levels of recombinant proteins Yoshi Hashimoto, Sheng Zhang, Shiying Zhang, Yun-Ru Chen, Gary W Blissard BMC Biotechnology 2012, 12:12 (24 April 2012) Abstract \| Full text \| PDF \| PubMed
		Methodology article Dual-lifetime referencing (DLR): a powerful method for on-line measurement of internal pH in carrier-bound immobilized biocatalysts Caterina Boniello, Torsten Mayr, Juan M Bolivar, Bernd Nidetzky BMC Biotechnology 2012, 12:11 (28 March 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary A Dual-Lifetime Referencing (DLR) method based on the luminophores fluorescein and Ru(dpp) allows the measurement of pH within the particles of carrier-bound immobilized enzymes and has potential as an analytical tool in biocatalysis.
		Methodology article A novel PCR-based method for high throughput prokaryotic expression of antimicrobial peptide genes Tao Ke, Su Liang, Jin Huang, Han Mao, Jibao Chen, Caihua Dong, Junyan Huang, Shengyi Liu, Jianxiong Kang, Dongqi Liu, Xiangdong Ma BMC Biotechnology 2012, 12:10 (23 March 2012) Abstract \| Full text \| PDF \| PubMed
		Research article The Bxb1 recombination system demonstrates heritable transmission of site-specific excision in Arabidopsis James G Thomson, Ronald Chan, Jamison Smith, Roger Thilmony, Yuan-Yeu Yau, YueJu Wang, David W Ow BMC Biotechnology 2012, 12:9 (21 March 2012) Abstract \| Full text \| PDF \| PubMed
		Research article A designer hyper interleukin 11 (H11) is a biologically active cytokine Hanna Dams-Kozlowska, Katarzyna Gryska, Eliza Kwiatkowska-Borowczyk, Dariusz Izycki, Stefan Rose-John, Andrzej Mackiewicz BMC Biotechnology 2012, 12:8 (21 March 2012) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
		Research article M cell targeting by a Claudin 4 targeting peptide can enhance mucosal IgA responses David D Lo, Jun Ling, A Holly Eckelhoefer BMC Biotechnology 2012, 12:7 (13 March 2012) Abstract \| Full text \| PDF \| PubMed
		Commentary Armed and accurate: engineering cytotoxic T cells for eradication of leukemia Marko Radic BMC Biotechnology 2012, 12:6 (8 February 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Marko Radic discusses recent advances in cancer immunotherapy, whereby cytotoxic T cells from patients with leukemia are turned into efficient and specific killers of their own cancer cells.
		Methodology article An improved method for isolation of RNA from bone Lauren E Carter, Gail Kilroy, Jeffrey M Gimble, Z Elizabeth Floyd BMC Biotechnology 2012, 12:5 (19 January 2012) Abstract \| Full text \| PDF \| PubMed \| 1 comment
		Research article Comparison and transfer testing of multiplex ligation detection methods for GM plants Gabriella Ujhelyi, Jeroen P van Dijk, Theo W Prins, Marleen M Voorhuijzen, AM Angeline Van Hoef, Henriek G Beenen, Dany Morisset, Kristina Gruden, Esther J Kok BMC Biotechnology 2012, 12:4 (19 January 2012) Abstract \| Full text \| PDF \| PubMed
		Methodology article Creation and validation of a ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells Asmita Patel, Anisleidys Muñoz, Katherine Halvorsen, Priyamvada Rai BMC Biotechnology 2012, 12:3 (16 January 2012) Abstract \| Full text \| PDF \| PubMed
		Research article Construction and transformation of a Thermotoga-E. coli shuttle vector Dongmei Han, Stephen M Norris, Zhaohui Xu BMC Biotechnology 2012, 12:2 (6 January 2012) Abstract \| Full text \| PDF \| PubMed
		Methodology article Single/low-copy integration of transgenes in Caenorhabditis elegans using an ultraviolet trimethylpsoralen method Eriko Kage-Nakadai, Hiroyuki Kobuna, Osamu Funatsu, Muneyoshi Otori, Keiko Gengyo-Ando, Sawako Yoshina, Sayaka Hori, Shohei Mitani BMC Biotechnology 2012, 12:1 (5 January 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary A method utilizing ultraviolet trimethylpsoralen (UV/TMP) based on familiar extrachromosomal transgenics offers an alternative to Mos1 transposon or microparticle bombardment in the creation of single- or low-copy chromosomal integrated lines of Caenorhabditis elegans.
		Research article Antibody degradation in tobacco plants: a predominantly apoplastic process Verena K Hehle, Matthew J Paul, Pascal M Drake, Julian KC Ma, Craig J van Dolleweerd BMC Biotechnology 2011, 11:128 (30 December 2011) Abstract \| Full text \| PDF \| PubMed
		Research article Incorporation of albumin fusion proteins into fibrin clots in vitro and in vivo: comparison of different fusion motifs recognized by factor XIIIa William P Sheffield, Louise J Eltringham-Smith BMC Biotechnology 2011, 11:127 (20 December 2011) Abstract \| Full text \| PDF \| PubMed
		Research article In situ gastrointestinal protection against anthrax edema toxin by single-chain antibody fragment producing lactobacilli Kasper Andersen, Harold Marcotte, Beatriz Álvarez, Prosper N Boyaka, Lennart Hammarström BMC Biotechnology 2011, 11:126 (20 December 2011) Abstract \| Full text \| PDF \| PubMed
		Research article Transmission electron microscopy characterization of fluorescently labelled amyloid β 1-40 and α-synuclein aggregates Valerie L Anderson, Watt W Webb BMC Biotechnology 2011, 11:125 (19 December 2011) Abstract \| Full text \| PDF \| PubMed
		Methodology article Real-time PCR-based assay to quantify the relative amount of human and mouse tissue present in tumor xenografts Sergio Y Alcoser, David J Kimmel, Suzanne D Borgel, John P Carter, Kelly M Dougherty, Melinda G Hollingshead BMC Biotechnology 2011, 11:124 (16 December 2011) Abstract \| Full text \| PDF \| PubMed
		Research article Adsorption characteristics of an enteric virus-binding protein to norovirus, rotavirus and poliovirus Takahiro Imai, Daisuke Sano, Takayuki Miura, Satoshi Okabe, Keishi Wada, Yoshifumi Masago, Tatsuo Omura BMC Biotechnology 2011, 11:123 (16 December 2011) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary A broad spectrum virus-binding protein derived from the bacterial chaperon protein GroEL and able to bind rotavirus, norovirus and poliovirus, could be used to detect diverse enteric viruses simultaneously in water and fecal samples.
		Methodology article Targeted sequencing library preparation by genomic DNA circularization Samuel Myllykangas, Georges Natsoulis, John M Bell, Hanlee P Ji BMC Biotechnology 2011, 11:122 (14 December 2011) Abstract \| Full text \| PDF \| PubMed
		Methodology article Combining M-FISH and Quantum Dot technology for fast chromosomal assignment of transgenic insertions Mohammed Yusuf, David LV Bauer, Daniel M Lipinski, Robert E MacLaren, Richard Wade-Martins, Kalim U Mir, Emanuela V Volpi BMC Biotechnology 2011, 11:121 (13 December 2011) Abstract \| Full text \| PDF \| PubMed
		Methodology article GAL1-SceI directed site-specific genomic (gsSSG) mutagenesis: a method for precisely targeting point mutations in S. cerevisiae Sarah Piccirillo, Hsiao-Lin Wang, Thomas J Fisher, Saul M Honigberg BMC Biotechnology 2011, 11:120 (5 December 2011) Abstract \| Full text \| PDF \| PubMed