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Volume 10 (2010) - October 2010

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Research article   Open Access

Creation of a novel peptide with enhanced nuclear localization in prostate and pancreatic cancer cell lines

H Dan Lewis, Ali Husain, Robert J Donnelly, Dimitrios Barlos, Sheraz Riaz, Kalyani Ginjupalli, Adetola Shodeinde, Beverly E Barton BMC Biotechnology 2010, 10:79 (28 October 2010)

Abstract | Full text | PDF | PubMed

Methodology article   Open Access Highly Accessed

A miniaturized sandwich immunoassay platform for the detection of protein-protein interactions

Qiongming Liu, Qing Chen, Jian Wang, Ying Zhang, Ying Zhou, Cong Lin, Wei He, Fuchu He, Danke Xu BMC Biotechnology 2010, 10:78 (28 October 2010)

Abstract | Full text | PDF | PubMed

Research article   Open Access Highly Accessed

Intranasal vaccination with messenger RNA as a new approach in gene therapy: Use against tuberculosis

Julio CC Lorenzi, Ana PF Trombone, Carolina D Rocha, Luciana P Almeida, Ricardo L Lousada, Thiago Malardo, Isabela C Fontoura, Renata AM Rossetti, Ana F Gembre, Aristóbolo M Silva, Celio L Silva, Arlete AM Coelho-Castelo BMC Biotechnology 2010, 10:77 (20 October 2010)

Abstract | Full text | PDF | PubMed

Research article   Open Access

Controlled expression of functional miR-122 with a ligand inducible expression system

Cathy M Shea, George Tzertzinis BMC Biotechnology 2010, 10:76 (20 October 2010)

Abstract | Full text | PDF | PubMed |  Editor’s summary

A new expression system relying on modified nuclear receptors and a synthetic ligand allows the construction of stable cell lines where the expression of miRNA or siRNA can be tightly and reversibly controlled.

Methodology article   Open Access Highly Accessed

Analysis of conditional gene deletion using probe based Real-Time PCR

Britta Weis, Joachim Schmidt, Frank Lyko, Heinz G Linhart BMC Biotechnology 2010, 10:75 (15 October 2010)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

Methodology article   Open Access

Multiplexed, rapid detection of H5N1 using a PCR-free nanoparticle-based genomic microarray assay

Jiangqin Zhao, Shixing Tang, James Storhoff, Sudhakar Marla, Y Paul Bao, Xue Wang, Eric Y Wong, Viswanath Ragupathy, Zhiping Ye, Indira K Hewlett BMC Biotechnology 2010, 10:74 (13 October 2010)

Abstract | Full text | PDF | PubMed

Research article   Open Access

Overexpression of biotin synthase and biotin ligase is required for efficient generation of sulfur-35 labeled biotin in E. coli

Teegan A Delli-Bovi, Maroya D Spalding, Sean T Prigge BMC Biotechnology 2010, 10:73 (11 October 2010)

Abstract | Full text | PDF | PubMed

Research article   Open Access Highly Accessed

Development of an EGFRvIII specific recombinant antibody

Puja Gupta, Shuang-Yin Han, Marina Holgado-Madruga, Siddhartha S Mitra, Gordon Li, Ryan T Nitta, Albert J Wong BMC Biotechnology 2010, 10:72 (7 October 2010)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

Methodology article   Open Access

Novel concept microarray enabling PCR and multistep reactions through pipette-free aperture-to-aperture parallel transfer

Yasunori Kinoshita, Takahiro Tayama, Koichiro Kitamura, Md Salimullah, Hidekazu Uchida, Miho Suzuki, Yuzuru Husimi, Koichi Nishigaki BMC Biotechnology 2010, 10:71 (6 October 2010)

Abstract | Full text | PDF | PubMed


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