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BMC Biotechnology

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Editor-in-Chief
Melissa Norton, MD

Biology Editor
Penelope Webb, PhD

In-house Editor
J. Ann Le Good PhD




BMC Biotechnology, Volume 9

 

 

 

Methodology article
A rapid and inexpensive labeling method for microarray gene expression analysis
Mario Ouellet, Paul D Adams, Jay D Keasling, Aindrila Mukhopadhyay
BMC Biotechnology 2009, 9:97 (25November2009)
[Abstract] [Provisional PDF] [PubMed] [Related articles]

Research article
High-level HIV-1 Nef transient expression in Nicotiana benthamiana using the P19 gene silencing suppressor protein of Artichoke Mottled Crinckle Virus
Raffaele Lombardi, Patrizia Circelli, Maria Elena Villani, Giampaolo Buriani, Luca Nardi, Valentina Coppola, Linda Bianco, Eugenio Benvenuto, Marcello Donini, Carla Marusic
BMC Biotechnology 2009, 9:96 (20November2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Quantifying and resolving multiple vector transformants in S. cerevisiae plasmid libraries
Thomas C Scanlon, Elizabeth C Gray, Karl E Griswold
BMC Biotechnology 2009, 9:95 (20November2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Non-antibiotic selection systems for soybean somatic embryos: the lysine analog aminoethyl-cysteine as a selection agent
Suryadevara S Rao, Lewamy Mamadou, Matt McConnell, Raghuveer Polisetty, Prachuab Kwanyuen, David Hildebrand
BMC Biotechnology 2009, 9:94 (18November2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Genetic load and transgenic mitigating genes in transgenic Brassica rapa (field mustard) Brassica napus (oilseed rape) hybrid populations
Christy W Rose, Reginald J Millwood, Hong S Moon, Murali R Rao, Matthew D Halfhill, Paul L Raymer, Suzanne I Warwick, Hani Al-Ahmad, Jonathan Gressel, C Neal Stewart Jr
BMC Biotechnology 2009, 9:93 (31October2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Affinity maturation generates greatly improved xyloglucan-specific carbohydrate binding modules
Laura von Schantz, Fredrika Gullfot, Sebastian Scheer, Lada Filonova, Lavinia Cicortas Gunnarsson, James E Flint, Geoffrey Daniel, Eva Nordberg-Karlsson, Harry Brumer, Mats Ohlin
BMC Biotechnology 2009, 9:92 (31October2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Authentication scheme for routine verification of genetically similar laboratory colonies: a trial with Anopheles gambiae
Elien E Wilkins, Paula L Marcet, Alice C Sutcliffe, Paul I Howell
BMC Biotechnology 2009, 9:91 (22October2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Using a water-immiscible ionic liquid to improve asymmetric reduction of 4-(trimethylsilyl)-3-butyn-2-one catalyzed by immobilized Candida parapsilosis CCTCC M203011 cells
Wen-Yong Lou, Lei Chen, Bo-Bo Zhang, Thomas J Smith, Min-Hua Zong
BMC Biotechnology 2009, 9:90 (22October2009)
[Abstract] [Provisional PDF] [PubMed] [Related articles]

Research article
Novel immortalized human fetal liver cell line, cBAL111, has the potential to differentiate into functional hepatocytes
Tanja Deurholt, Niek P van Til, Aniska A Chhatta, Lysbeth ten Bloemendaal, Ruth Schwartlander, Catherine Payne, John N Plevris, Igor M Sauer, Robert AFM Chamuleau, Ronald PJ Oude Elferink, Jurgen Seppen, Ruurdtje Hoekstra
BMC Biotechnology 2009, 9:89 (21October2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Regulated expression of a transgene introduced on an oriP/EBNA-1 PAC shuttle vector into human cells
Hanne A Askautrud, Elisabet Gjernes, Gro L Strvold, Mona M Lindeberg, Jim Thorsen, Hans Prydz, Eirik Frengen
BMC Biotechnology 2009, 9:88 (16October2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Characterization of unknown genetic modifications using high throughput sequencing and computational subtraction
Torstein Tengs, Haibo Zhang, Arne Holst-Jensen, Jon Bohlin, Melinka A Butenko, Anja Brthen Kristoffersen, Hilde-Gunn Opsahl Sorteberg, Knut G Berdal
BMC Biotechnology 2009, 9:87 (8October2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Refinement of lentiviral vector for improved RNA processing and reduced rates of self inactivation repair
Rachel M Koldej, Donald S Anson
BMC Biotechnology 2009, 9:86 (7October2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Reduction of liver macrophage transduction by pseudotyping lentiviral vectors with a fusion envelope from Autographa californica GP64 and Sendai virus F2 domain
David M Markusic, Niek P van Til, Johan K Hiralall, Ronald PJ Oude Elferink, Jurgen Seppen
BMC Biotechnology 2009, 9:85 (7October2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Thermochemotherapy effect of nanosized As2O3/Fe3O4 complex on experimental mouse tumors and its influence on the expression of CD44v6, VEGF-C and MMP-9
Yiqun Du, Dongsheng Zhang, Hui Liu, Rensheng Lai
BMC Biotechnology 2009, 9:84 (5October2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
MALS: an efficient strategy for multiple site-directed mutagenesis employing a combination of DNA amplification, ligation and suppression PCR
Alexey A Fushan, Dennis T Drayna
BMC Biotechnology 2009, 9:83 (24September2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Integrating human stem cell expansion and neuronal differentiation in bioreactors
Margarida Serra, Catarina Brito, Eunice M Costa, Marcos FQ Sousa, Paula M Alves
BMC Biotechnology 2009, 9:82 (22September2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
A combination of transposable elements and magnetic cell sorting provides a very efficient transgenesis system for chicken primary erythroid progenitors
Camila Mejia-Pous, Jos Viuelas, Claudine Faure, Joanna Koszela, Koichi Kawakami, Yoshiko Takahashi, Olivier Gandrillon
BMC Biotechnology 2009, 9:81 (18September2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Monodispersity of recombinant Cre recombinase correlates with its effectiveness in vivo
Paola Capasso, Marisa Aliprandi, Giuseppe Ossolengo, Frank Edenhofer, Ario de Marco
BMC Biotechnology 2009, 9:80 (11September2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Antibody expressing pea seeds as fodder for prevention of gastrointestinal parasitic infections in chickens
Jana Zimmermann, Isolde Saalbach, Doreen Jahn, Martin Giersberg, Sigrun Haehnel, Julia Wedel, Jeanette Macek, Karen Zoufal, Gerhard Glnder, Dieter Falkenburg, Sergey M Kipriyanov
BMC Biotechnology 2009, 9:79 (11September2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [1 comment]

Research article
A comparative study of recombinant and native frutalin binding to human prostate tissues
Carla Oliveira, Jos A Teixeira, Fernando Schmitt, Luclia Domingues
BMC Biotechnology 2009, 9:78 (9September2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
New stably transfected bioluminescent cells expressing FLAG epitope-tagged estrogen receptors to study their chromatin recruitment
Eric Badia, Aurlie Escande, Patrick Balaguer, Raphal Mtivier, Vincent Cavailles
BMC Biotechnology 2009, 9:77 (9September2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
cMyc increases cell number through uncoupling of cell division from cell size in CHO cells
Darrin Kuystermans, Mohamed Al-Rubeai
BMC Biotechnology 2009, 9:76 (7September2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Evaluation of the impact of single nucleotide polymorphisms and primer mismatches on quantitative PCR
Brian Boyle, Nancy Dallaire, John MacKay
BMC Biotechnology 2009, 9:75 (28August2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Directed evolution of a filamentous fungus for thermotolerance
Eudes de Crecy, Stefan Jaronski, Benjamin Lyons, Thomas J Lyons, Nemat O Keyhani
BMC Biotechnology 2009, 9:74 (26August2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Transduction of the MPG-tagged fusion protein into mammalian cells and oocytes depends on amiloride-sensitive endocytic pathway
So-Jung Kwon, Kyuyong Han, Suhyun Jung, Jong-Eun Lee, Seongsoon Park, Yong-Pil Cheon, Hyunjung Jade Lim
BMC Biotechnology 2009, 9:73 (26August2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
High quality protein microarray using in situ protein purification
Keehwan Kwon, Carissa Grose, Rembert Pieper, Gagan A Pandya, Robert D Fleischmann, Scott N Peterson
BMC Biotechnology 2009, 9:72 (23August2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Archaeosomes made of Halorubrum tebenquichense total polar lipids: a new source of adjuvancy
Raul O Gonzalez, Leticia H Higa, Romina A Cutrullis, Marcos Bilen, Irma Morelli, Diana I Roncaglia, Ricardo S Corral, Maria Jose Morilla, Patricia B Petray, Eder L Romero
BMC Biotechnology 2009, 9:71 (13August2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Efficient production of human bivalent and trivalent anti-MUC1 Fab-scFv antibodies in Pichia pastoris
Steve Schoonooghe, Vladimir Kaigorodov, Monika Zawisza, Caroline Dumolyn, Jurgen Haustraete, Johan Grooten, Nico Mertens
BMC Biotechnology 2009, 9:70 (11August2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Efficient generation of long-distance conditional alleles using recombineering and a dual selection strategy in replicate plates
David Voehringer, Davina Wu, Hong-Erh Liang, Richard M Locksley
BMC Biotechnology 2009, 9:69 (28July2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Evaluation of cross-linked aggregates from purified Bacillus subtilis levansucrase mutants for transfructosylation reactions
Maria Elena Ortiz-Soto, Enrique Rudio-Piera, Maria Elena Rodriguez-Alegria, Agustin Lopez Munguia
BMC Biotechnology 2009, 9:68 (27July2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Gamma-phage lysin PlyG sequence-based synthetic peptides coupled with Qdot-nanocrystals are useful for developing detection methods for Bacillus anthracis by using its surrogates, B. anthracis-Sterne and B. cereus-4342
Shilpakala Sainathrao, Ketha V Krishna Mohan, Chintamani Atreya
BMC Biotechnology 2009, 9:67 (22July2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Efficient, chemoselective synthesis of immunomicelles using single-domain antibodies with a C-terminal thioester
Sanne WA Reulen, Ingrid van Baal, Jos MH Raats, Maarten Merkx
BMC Biotechnology 2009, 9:66 (20July2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
A novel Poly(-caprolactone)-Pluronic-Poly(-caprolactone) grafted Polyethyleneimine(PCFC-g-PEI), Part 1, synthesis, cytotoxicity, and in vitro transfection study
Shuai Shi, QingFa Guo, Bing Kan, ShaoZhi Fu, XiuHong Wang, ChangYang Gong, HongXin Deng, Feng Luo, Xia Zhao, YuQuan Wei, ZhiYong Qian
BMC Biotechnology 2009, 9:65 (17July2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Stable nuclear transformation of Gonium pectorale
Kai Lerche, Armin Hallmann
BMC Biotechnology 2009, 9:64 (10July2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Production of N-acetyl-D-neuraminic acid using two sequential enzymes overexpressed as double-tagged fusion proteins
Tzu-Hsien Wang, Ying-Yin Chen, Hsin-Hung Pan, Feng-Pao Wang, Chung-Hsien Cheng, Wen-Chien Lee
BMC Biotechnology 2009, 9:63 (9July2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
A further insight into the biosorption mechanism of Pt(IV) by infrared spectrometry
Zhongyu Lin, Ru Xue, Yiwen Ye, Jianhong Zheng, Zhenling Xu
BMC Biotechnology 2009, 9:62 (6July2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies
Oded Edelheit, Aaron Hanukoglu, Israel Hanukoglu
BMC Biotechnology 2009, 9:61 (30June2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Isolation of a human-like antibody fragment (scFv) that neutralizes ricin biological activity
Thibaut Pelat, Michael Hust, Martha Hale, Marie-Paule Lefranc, Stefan Dbel, Philippe Thullier
BMC Biotechnology 2009, 9:60 (30June2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Novel sequential ChIP and simplified basic ChIP protocols for promoter co-occupancy and target gene identification in human embryonic stem cells
Ricardo B Medeiros, Kate J Papenfuss, Brian Hoium, Kristen Coley, Joy Jadrich, Saik-Kia Goh, Anuratha Elayaperumal, Julio E Herrera, Ernesto Resnik, Hsiao-Tzu Ni
BMC Biotechnology 2009, 9:59 (29June2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Cell-free protein synthesis energized by slowly-metabolized maltodextrin
Yiran Wang, Y-H Percival Zhang
BMC Biotechnology 2009, 9:58 (28June2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Interfering ribonucleic acids that suppress expression of multiple unrelated genes
Toby Passioura, Mary M Gozar, Amber Goodchild, Andrew King, Greg M Arndt, Michael Poidinger, Donald J Birkett, Laurent P Rivory
BMC Biotechnology 2009, 9:57 (16June2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Expression of endo-1, 4-beta-xylanase from Trichoderma reesei in Pichia pastoris and functional characterization of the produced enzyme
Jun He, Bing Yu, Keying Zhang, Xuemei Ding, Daiwen Chen
BMC Biotechnology 2009, 9:56 (16June2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
High-titer preparation of Bombyx mori nucleopolyhedrovirus (BmNPV) displaying recombinant protein in silkworm larvae by size exclusion chromatography and its characterization
Tatsuya Kato, Suganthi Lavender Manoha, Shigeyasu Tanaka, Enoch Y Park
BMC Biotechnology 2009, 9:55 (12June2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Synthesis of sialoglycopolypeptide for potentially blocking influenza virus infection using a rat 2,6-sialyltransferase expressed in BmNPV bacmid-injected silkworm larvae
Makoto Ogata, Makoto Nakajima, Tatsuya Kato, Takakiyo Obara, Hirokazu Yagi, Koichi Kato, Taichi Usui, Enoch Y Park
BMC Biotechnology 2009, 9:54 (5June2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Construction of siRNA/miRNA expression vectors based on a one-step PCR process
Jun Xu, Jie Qiong Zeng, Gang Wan, Gui Bin Hu, Hong Yan, Li Xin Ma
BMC Biotechnology 2009, 9:53 (2June2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Integrated application of uniform design and least-squares support vector machines to transfection optimization
Jin-Shui Pan, Mei-Zhu Hong, Qi-Feng Zhou, Jia-Yan Cai, Hua-Zhen Wang, Lin-Kai Luo, De-Qiang Yang, Jing Dong, Hua-Xiu Shi, Jian-Lin Ren
BMC Biotechnology 2009, 9:52 (31May2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
A microarray-based method for the parallel analysis of genotypes and expression profiles of wood-forming tissues in Eucalyptus grandis
Eugenia Barros, Carol-Ann van Staden, Sabine Lezar
BMC Biotechnology 2009, 9:51 (27May2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Cost-effective HRMA pre-sequence typing of clone libraries; application to phage display selection
Barry A Pepers, Menno H Schut, Rolf HAM Vossen, Gert-Jan B van Ommen, Johan T den Dunnen, Willeke MC van Roon-Mom
BMC Biotechnology 2009, 9:50 (22May2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Expanding the multicolor capabilities of basic confocal microscopes by employing red and near-infrared quantum dot conjugates
Lara M Kingeter, Brian C Schaefer
BMC Biotechnology 2009, 9:49 (22May2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Peroxicretion: a novel secretion pathway in the eukaryotic cell
Cees MJ Sagt, Peter J ten Haaft, Ingeborg M Minneboo, Miranda P Hartog, Robbert A Damveld, Jan Metske van der Laan, Michiel Akeroyd, Thibaut J Wenzel, Francisca A Luesken, Marten Veenhuis, Ida van der Klei, Johannes H de Winde
BMC Biotechnology 2009, 9:48 (20May2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [F1000 Biology]

Methodology article
Generation of an external guide sequence library for a reverse genetic screen in Caenorhabditis elegans
Qitao Yan, Rui Zhao, Wenlin Zheng, Changxin Yin, Bao Zhang, Wenli Ma
BMC Biotechnology 2009, 9:47 (20May2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Luciferase activity under direct ligand-dependent control of a muscarinic acetylcholine receptor
Doreen Thor, Diana Le Duc, Rainer Strotmann, Torsten Schneberg
BMC Biotechnology 2009, 9:46 (18May2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Fluorescent labeling of NASBA amplified tmRNA molecules for microarray applications
Ott Scheler, Barry Glynn, Sven Parkel, Priit Palta, Kadri Toome, Lauris Kaplinski, Maido Remm, Majella Maher, Ants Kurg
BMC Biotechnology 2009, 9:45 (15May2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
RNAi in the cereal weevil Sitophilus spp: Systemic gene knockdown in the bacteriome tissue
Agns Vallier, Carole Vincent-Mongat, Anne Laurenon, Abdelaziz Heddi
BMC Biotechnology 2009, 9:44 (15May2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
The CD-loop of PAI-2 (SERPINB2) is redundant in the targeting, inhibition and clearance of cell surface uPA activity
Blake J Cochran, Lakshitha P Gunawardhana, Kara L Vine, Jodi A Lee, Sergei Lobov, Marie Ranson
BMC Biotechnology 2009, 9:43 (14May2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Fluorescent labeling in semi-solid medium for selection of mammalian cells secreting high-levels of recombinant proteins
Antoine W Caron, Claire Nicolas, Bruno Gaillet, Ismala Ba, Maxime Pinard, Alain Garnier, Bernard Massie, Rnald Gilbert
BMC Biotechnology 2009, 9:42 (11May2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
A suite of recombinant luminescent bacterial strains for the quantification of bioavailable heavy metals and toxicity testing
Angela Ivask, Taisia Rlova, Anne Kahru
BMC Biotechnology 2009, 9:41 (8May2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Efficient CPP-mediated Cre protein delivery to developing and adult CNS tissues
Yorick Gitton, Lorenzo Tibaldi, Edmond Dupont, Giovanni Levi, Alain Joliot
BMC Biotechnology 2009, 9:40 (24April2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
A modified and automated version of the 'Fluorimetric Detection of Alkaline DNA Unwinding' method to quantify formation and repair of DNA strand breaks
Mara Moreno-Villanueva, Ragen Pfeiffer, Thilo Sindlinger, Alan Leake, Marcus Mller, Thomas BL Kirkwood, Alexander Brkle
BMC Biotechnology 2009, 9:39 (23April2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Acetylation of PAMAM dendrimers for cellular delivery of siRNA
Carolyn L Waite, Sarah M Sparks, Kathryn E Uhrich, Charles M Roth
BMC Biotechnology 2009, 9:38 (23April2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Combined protein construct and synthetic gene engineering for heterologous protein expression and crystallization using Gene Composer
Amy Raymond, Scott Lovell, Don Lorimer, John Walchli, Mark Mixon, Ellen Wallace, Kaitlin Thompkins, Kimberly Archer, Alex Burgin, Lance Stewart
BMC Biotechnology 2009, 9:37 (21April2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Software
Gene Composer: database software for protein construct design, codon engineering, and gene synthesis
Don Lorimer, Amy Raymond, John Walchli, Mark Mixon, Adrienne Barrow, Ellen Wallace, Rena Grice, Alex Burgin, Lance Stewart
BMC Biotechnology 2009, 9:36 (21April2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Research article
A comparison of synthetic oligodeoxynucleotides, DNA fragments and AAV-1 for targeted episomal and chromosomal gene repair
Xavier Leclerc, Olivier Danos, Daniel Scherman, Antoine Kichler
BMC Biotechnology 2009, 9:35 (20April2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Collagen matrices from sponge to nano: new perspectives for tissue engineering of skeletal muscle
Justus P Beier, Dorothee Klumpp, Markus Rudisile, Roland Dersch, Joachim H Wendorff, Oliver Bleiziffer, Andreas Arkudas, Elias Polykandriotis, Raymund E Horch, Ulrich Kneser
BMC Biotechnology 2009, 9:34 (15April2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Optimization of codon composition and regulatory elements for expression of human insulin like growth factor-1 in transgenic chloroplasts and evaluation of structural identity and function
Henry Daniell, Gricel Ruiz, Bela Denes, Laurence Sandberg, William Langridge
BMC Biotechnology 2009, 9:33 (3April2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Noncytotoxic orange and red/green derivatives of DsRed-Express2 for whole-cell labeling
Rita L Strack, Dibyendu Bhattacharyya, Benjamin S Glick, Robert J Keenan
BMC Biotechnology 2009, 9:32 (3April2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
PhiC31 integrase induces a DNA damage response and chromosomal rearrangements in human adult fibroblasts
Jian Liu, Tina Skjrringe, Torben Gjetting, Thomas G Jensen
BMC Biotechnology 2009, 9:31 (2April2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Simultaneous detection of mRNA and protein stem cell markers in live cells
Won Jong Rhee, Gang Bao
BMC Biotechnology 2009, 9:30 (2April2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Vaccination with recombinant Boophilus annulatus Bm86 ortholog protein, Ba86, protects cattle against B. annulatus and B. microplus infestations
Mario Canales, Consuelo Almazn, Victoria Naranjo, Frans Jongejan, Jos de la Fuente
BMC Biotechnology 2009, 9:29 (31March2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
AC133+ progenitor cells as gene delivery vehicle and cellular probe in subcutaneous tumor models: a preliminary study
Ali M Rad, ASM Iskander, Branislava Janic, Robert A Knight, Ali S Arbab, Hamid Soltanian-Zadeh
BMC Biotechnology 2009, 9:28 (27March2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Folding machineries displayed on a cation-exchanger for the concerted refolding of cysteine- or proline-rich proteins
Dae-Hee Lee, Sung-Gun Kim, Dae-Hyuk Kweon, Jin-Ho Seo
BMC Biotechnology 2009, 9:27 (26March2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Accumulation and processing of a recombinant protein designed as a cleavable fusion to the endogenous Rubisco LSU protein in Chlamydomonas chloroplast
Machiko Muto, Ryan E Henry, Stephen P Mayfield
BMC Biotechnology 2009, 9:26 (26March2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Correspondence
Effects of research tool patents on biotechnology innovation in a developing country: A case study of South Korea
Kyung-Nam Kang, Tae-Kyu Ryu, Yoon-Sik Lee
BMC Biotechnology 2009, 9:25 (26March2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Practical and reliable FRET/FLIM pair of fluorescent proteins
Dmitry Shcherbo, Ekaterina A Souslova, Joachim Goedhart, Tatyana V Chepurnykh, Anna Gaintzeva, Irina I Shemiakina, Theodorus WJ Gadella, Sergey Lukyanov, Dmitriy M Chudakov
BMC Biotechnology 2009, 9:24 (25March2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Adenoviral vectors for highly selective gene expression in central serotonergic neurons reveal quantal characteristics of serotonin release in the rat brain
Kheira Benzekhroufa, Beihui Liu, Feige Tang, Anja G Teschemacher, Sergey Kasparov
BMC Biotechnology 2009, 9:23 (19March2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
Viral and murine interleukin-10 are correctly processed and retain their biological activity when produced in tobacco
Luisa Bortesi, Marzia Rossato, Flora Schuster, Nicole Raven, Johannes Stadlmann, Linda Avesani, Alberto Falorni, Flavia Bazzoni, Ralph Bock, Stefan Schillberg, Mario Pezzotti
BMC Biotechnology 2009, 9:22 (19March2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles] [Cited on BioMed Central]

Research article
Chromato-panning: an efficient new mode of identifying suitable ligands from phage display libraries
Wim Noppe, Fatima Plieva, Igor Yu Galaev, Hans Pottel, Hans Deckmyn, Bo Mattiasson
BMC Biotechnology 2009, 9:21 (17March2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
A BAC-bacterial recombination method to generate physically linked multiple gene reporter DNA constructs
Peter Maye, Mary Louise Stover, Yaling Liu, David W Rowe, Shiaoching Gong, Alexander C Lichtler
BMC Biotechnology 2009, 9:20 (13March2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
An optimised system for refolding of human glucose 6-phosphate dehydrogenase
Xiao-Tao Wang, Paul C Engel
BMC Biotechnology 2009, 9:19 (11March2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
A novel method for producing target cells and assessing cytotoxic T lymphocyte activity in outbred hosts
Francesca Bonci, Elisa Zabogli, Francesca Conti, Antonio Merico, Giulia Freer, Mauro Bendinelli, Mauro Pistello
BMC Biotechnology 2009, 9:18 (11March2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Research article
The properties of bioengineered chondrocyte sheets for cartilage regeneration
Genya Mitani, Masato Sato, Jeong IK Lee, Nagatoshi Kaneshiro, Miya Ishihara, Naoshi Ota, Mami Kokubo, Hideaki Sakai, Tetsutaro Kikuchi, Joji Mochida
BMC Biotechnology 2009, 9:17 (6March2009)
[Abstract] [Full text] [PDF] [PubMed] [Related articles]

Methodology article
Real-time quantification of wild-type contaminants in glyphosate tolerant soybean
Elena Battistini, Enrico Noli
BMC Biotechnology 2009, 9:16 (6March2009)
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Research article
Addition of a sequence from 2-antiplasmin transforms human serum albumin into a blood clot component that speeds clot lysis
William P Sheffield, Louise J Eltringham-Smith, Sharon Gataiance, Varsha Bhakta
BMC Biotechnology 2009, 9:15 (3March2009)
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Sandrine Moutel, Ahmed El Marjou, Ole Vielemeyer, Clment Nizak, Philippe Benaroch, Stefan Dbel, Franck Perez
BMC Biotechnology 2009, 9:14 (26February2009)
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Incorporating double copies of a chromatin insulator into lentiviral vectors results in less viral integrants
Troels T Nielsen, Johan Jakobsson, Nina Rosenqvist, Cecilia Lundberg
BMC Biotechnology 2009, 9:13 (24February2009)
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Improving the functional expression of a Bacillus licheniformis laccase by random and site-directed mutagenesis
Katja Koschorreck, Rolf D Schmid, Vlada B Urlacher
BMC Biotechnology 2009, 9:12 (23February2009)
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Noninvasive technique for measurement of heartbeat regularity in zebrafish (Danio rerio) embryos
Po Kwok Chan, Chun Chi Lin, Shuk Han Cheng
BMC Biotechnology 2009, 9:11 (19February2009)
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Methodology article
Simplified production and concentration of HIV-1-based lentiviral vectors using HYPERFlask vessels and anion exchange membrane chromatography
Robert H Kutner, Sharon Puthli, Michael P Marino, Jakob Reiser
BMC Biotechnology 2009, 9:10 (16February2009)
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Isolation of osteogenic progenitors from human amniotic fluid using a single step culture protocol
Ivana Antonucci, Irene Iezzi, Elisena Morizio, Filiberto Mastrangelo, Andrea Pantalone, Monica Mattioli-Belmonte, Antonio Gigante, Vincenzo Salini, Giuseppe Calabrese, Stefano Tet, Giandomenico Palka, Liborio Stuppia
BMC Biotechnology 2009, 9:9 (16February2009)
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In vitro drug release behavior from a novel thermosensitive composite hydrogel based on Pluronic f127 and poly(ethylene glycol)-poly(-caprolactone)-poly(ethylene glycol) copolymer
Chang Yang Gong, Shuai Shi, Peng Wei Dong, Xiu Ling Zheng, Shao Zhi Fu, Gang Guo, Jing Liang Yang, Yu Quan Wei, Zhi Yong Qian
BMC Biotechnology 2009, 9:8 (11February2009)
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Research article
Detection of genetically modified organisms (GMOs) using isothermal amplification of target DNA sequences
David Lee, Maurizio La Mura, Theo R Allnutt, Wayne Powell
BMC Biotechnology 2009, 9:7 (2February2009)
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A compact phage display human scFv library for selection of antibodies to a wide variety of antigens
Potjamas Pansri, Nanthnit Jaruseranee, Kuntalee Rangnoi, Peter Kristensen, Montarop Yamabhai
BMC Biotechnology 2009, 9:6 (29January2009)
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Research article
Silica-based cationic bilayers as immunoadjuvants
Nilton Lincopan, Mariana RA Santana, Eliana Faquim-Mauro, Maria Helena B da Costa, Ana M Carmona-Ribeiro
BMC Biotechnology 2009, 9:5 (19January2009)
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Research article
Gene therapy by electroporation for the treatment of chronic renal failure in companion animals
Patricia A Brown, Angela M Bodles-Brakhop, Melissa A Pope, Ruxandra Draghia-Akli
BMC Biotechnology 2009, 9:4 (16January2009)
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Methodology article
Bacterial artificial chromosomes improve recombinant protein production in mammalian cells
Leander Blaas, Monica Musteanu, Robert Eferl, Anton Bauer, Emilio Casanova
BMC Biotechnology 2009, 9:3 (14January2009)
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Research article
Albumin fibrillization induces apoptosis via integrin/FAK/Akt pathway
Chun-Yung Huang, Chi-Ming Liang, Chiao-Li Chu, Shu-Mei Liang
BMC Biotechnology 2009, 9:2 (8January2009)
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Research article
Reporter gene-expressing bone marrow-derived stromal cells are immune-tolerated following implantation in the central nervous system of syngeneic immunocompetent mice
Irene Bergwerf, Nathalie De Vocht, Bart Tambuyzer, Jacob Verschueren, Kristien Reekmans, Jasmijn Daans, Abdelilah Ibrahimi, Viggo Van Tendeloo, Shyama Chatterjee, Herman Goossens, Philippe G Jorens, Veerle Baekelandt, Dirk Ysebaert, Eric Van Marck, Zwi N Berneman, Annemie Van Der Linden, Peter Ponsaerts
BMC Biotechnology 2009, 9:1 (7January2009)
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