- Joaquim M Cabral, Instituto Superior Tecnico
- Lluis Montoliu, Centro Nacional de Biotecnologia
- Mats Nilsson, Uppsala University
- Peter J Punt, TNO Microbiology & Systems Biology/Leiden University
- Marko Radic, University of Tennessee
- John J Rossi, Beckman Research Institute City of Hope
- Igor Stagljar, University of Toronto
- Neal C Stewart, University of Tennessee
- Dafne Solera, BioMed Central
An optimized phage display vector design leads to the construction of two improved naive antibody gene libraries for the generation of human antibodies for diagnostics and therapy.
By mutating 10 amino acids an increase in the net charge of the enzyme was observed and resulted in a reduced autoactivation though activation by enteropeptidase was only slightly influenced.
Supercharging of human enteropeptidase light chain via site-directed mutagenesis greatly increases its solubility and the yield of enzyme-antibody coupling, which in turns improves assay sensitivity when used in enzyme-linked immunosorbent assays (ELISA).
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BMC Biotechnology is an open access, peer-reviewed journal that considers articles on the manipulation of biological macromolecules or organisms for use in experimental procedures, cellular and tissue engineering or in the pharmaceutical, agricultural biotechnology and allied industries.
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Section Editor's Profile
Neal Stewart is a Professor of Plant Sciences and holds the Ivan Racheff Chair of Excellence in plant molecular genetics at the University of Tennessee. He is the co-director of the Tennessee Plant Research Center and holds the position of Senior Bioscientist at Oak Ridge National Laboratory. He has served on the editorial board as an Associate Editor for BMC Biotechnology since 2005. In 2011 he started serving the journal as Section Editor for plant biotechnology. His research focuses on plant biotechnology for bioenergy, agricultural and environmental applications. His lab uses such tools from genomics to systems biology in plants. His latest book, "Research Ethics for Life Scientists" reflects a passion for teaching research integrity. Stewart is also a singer-songwriter who is trying to spend more time in Nashville.
"I love open access publishing and BMC Biotechnology. Open access gives the gift of research knowledge to the world. Open access journals such as BMC Biotechnology, have long been among my top choices to publish research from my lab"