Novel centrosomal protein that associates with microtubules and midbody
Interrogation and integration of online resources identified genes/proteins that display transcriptional co-expression or protein-protein interactions (PPI) with the core centromere/kinetochore components. Top-ranked hubs in either co-expression or PPI network are not only enriched with known mitosis regulators, but also contain candidates whose mitotic functions are not clearly established. Experimental validation found that KIAA1377 is a novel centrosomal protein that also associates with microtubules and midbody, while TRIP13 is a novel kinetochore protein and directly interacts with mitotic checkpoint silencing protein p31comet. GFP-KIAA1377 was confirmed to localize at the midbody during cytokinesis, co-localizing with microtubule bundles adjacent to two Plk1-containing discs. In cells showing relatively higher expression, GFP-KIAA1377 was also observed to extensively overlap with the microtubule network in interphase cells and the spindle in mitotic cells. Shown is a transfected interphase HeLa cell expressing GFP-KIAA1377 (green), also probed for alpha-tubulin (red) by immunofluorescence to indicate co-localization of GFPKIAA1377 with microtubule structures.
Taken from: Song-Tao Liu et al., 2012, BMC Cell Biology [View article]
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