SUN1 and torsinA interact at the nuclear envelopeThe torsin A (torA) AAA+ protein possesses a nuclear envelop (NE) localized activity that is known to require Lamina-Associated-Polypeptide 1 (LAP1). The deltaE variant of torA, which causes dystonia, an autosomal dominant neurological condition, is also often associated with the NE in spite of being unable to bind LAP1. SUN1, one of the SUN-domain and Nesprin proteins that assemble into the LINC complex, was found to associate with torA-deltaE, but evidence was also presented that SUN1 could be a normal partner of wild-type torA, distinct from LAP1, and that the deltaE mutation might stabilize an otherwise transient interaction between torA and SUN1. Indeed, the NE localization of wild-type torA was reduced by SUN1 siRNA treatment. Shown is the anti-GFP labeling of NIH-3T3 cells expressing GFP-torA (green) transfected with SUN1 siRNA. Anti-LaminA/C labeling (red) highlights the position of the NE.
Taken from: Rose Goodchild et al., 2011, BMC Cell Biology [View article]
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