siRNA mediated HMGA1 depletion in C2C12 cells
High mobility group A (HMGA) proteins regulate gene expression and are downregulated after induction of myogenesis in wild type C2C12 cells. This downregulation is crucial to start the myogenic program and sustained HMGA1 expression even prevents myogenesis. siRNA knockdown of HMGA1 promotes myogenesis, as visualized by cell fusion and myotube formation (red). Immunofluorescence localization of myosin (red; stained with an anti-myosin antibody) was performed in C2C12 cells on day 6 after induction. DNA was stained with Hoechst (blue).
This information is for Figure 7, part B
Taken from: Robert Hock et al., 2010, BMC Cell Biology [View article]
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