Organelle localization and biogenesis in developing C. elegans intestinal cells
In C. elegans, CUP-5 is required for biogenesis of lysosomes. cup-5 mutant embryos have enlarged yolk granules in the developing intestinal cells. Therefore, to examine possible roles of CUP-5 in lysosome and gut granule biogenesis, an initial question of whether these organelles were individual compartments had to be answered. Using wild-type transgenic worms (LMP-1::GFP), confocal visualization of lysosomes (green) was performed after immunostaining embryos for PGP-2 protein (red) to visualize gut granules. This confirmed that both of these organelles were discrete organelles in intestinal cells of wild type embryos.
This information is for Figure 1 part D.
Taken from: Hanna Fares et al., 2010, BMC Cell Biology [View article]
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