Cellular localization of Pelota with HAX1
Pelota (PELO) is known to be involved in regulating cell proliferation and stem cell self-renewal. To confirm that a PELO interacting partner (HAX1), identified by a yeast two hybrid screen, is a binding partner in live cells, a bimolecular fluorescence complementation assay was performed. Complementation of both non-fluorescent GFP halves resulted in functional fluorophores (green) on protein interaction. HeLa cells were transfected with PELO-GFPC and HAX1-GFPN and interaction was visualized by fluorescence on cytoskeletal filaments. Nuclei (blue) were stained with DAPI.
Taken from: Ibrahim Adham et al., 2010, BMC Cell Biology [View article]