3D nuclear organization of telomeres
The transition from mononuclear Hodgkin lymphoma cells to diagnostic multinuclear Reed-Sternberg (RS) cells is associated with the increase of very short telomeres including "t-stumps", telomere loss, telomeric aggregate formation and the generation of "ghost nuclei". The analysis of telomere dynamics by quantitative 3D FISH in the Hodgkin cell line U-HO1 and its non-receptor protein-tyrosine phosphatase N1 (PTPN1) stable transfectant U-HO1-PTPN1reveals that over-expression of PTPN1 promotes cellular arrest in G2, apoptosis, and the accumulation of RS cells that, in spite of the similarly high telomerase activity observed in both cell lines, show a market reduction in the number of very short telomeres. The 2D Z-stack image shows a ring-like multinuclear U-HO1-derived Reed-Sternberg cell composed of at least four nuclei of variable size (blue) punctuated by numerous telomeres (red dots).
This information is for Figure 6, part A
Taken from: Hans Knecht et al., 2010, BMC Cell Biology [View article]
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