The role of microtubules in the life cycle of autophagosomes
. Although it has been suggested that mature autophagosomes move along microtubular tracks, it is still in dispute if the earlier stages before and later stages after autophagosomal maturation require microtubules. Biochemical markers of autophagy were used in combination with microtubule interfering reagents to show that regular non-acetylated microtubules are involved in autophagosomal biogenesis whereas acetylated microtubules are required for the fusion of mature autophagosomes with lysosomes to form autolysosomes. Immunostaining of microtubules in HeLa cells with antibodies against acetylated α-tubulin (green) and normal β-tubulin (red) shows that the two sets of microtubular filaments coexist, with the acetylated microtubules concentrating in the perinuclear region.
This information is for Figure 3, part A
Taken from: Leyuan Liu et al., 2010, BMC Cell Biology [View article]
To view related images from across BioMed Central, visit the Biology Image Library.