Arp3 Localization during mitosis and cytokinesis
HeLa cells were enriched in mitosis by both thymidine and RO3306 blocks. Cells were permeabilized and fixed two hours after RO3306 release. Localization of the actin nucleation factor Arp3 (red) was shown by indirect staining with Alexa 555 and localization of beta-tubulin (blue) was shown with specific antibodies and Alexa 350. F-actin (green) was visualized with FITC-coupled phalloidin.
Taken from: Guillaume Bompard et al., 2008, BMC Cell Biology [View article]
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