Tight junction formation in the presence of heat shock protein Apg-2
Control canine kidney (MDCK) cells were grown in 3D-extracellular matrix gel cultures to form hollow polarized cysts and labeled with fluorescent antibodies for tight-junction and cytoskeletal proteins. Tight junctions were labeled with junctional marker occludin (red), the basolateral plasma membrane protein erbB-2 (green) and actin was labeled with Alexa654-phalloidin (blue). erbB-2 localized to the lateral plasma membrane as well as cytoplasmic organelles. The co-localization of occludin and actin appears pink.
Taken from: Karl Matter et al., 2007, BMC Cell Biology [View article]
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