Skip to main content

Advertisement

We are working on a new version of this page and we'd like your opinion. See an early preview or learn more.

Mosquitoes resistant to DDT are also resistant to mosquito net insecticide

A single genetic mutation causes resistance to DDT and pyrethroids (an insecticide class used in mosquito nets), according to new research in the journal Genome Biology. As resistance to pyrethroids and DDT is already spreading in mosquito populations, scientists say that this knowledge could help improve malaria control strategies.

The scientists used a wide range of methods to narrow down how the resistance works, finding a single mutation in the GSTe2 gene, which makes insects break down DDT so it’s no longer toxic. They have also shown that this gene makes insects resistant to pyrethroids raising the concern that GSTe2 gene could protect mosquitoes against the major insecticides used in public health.

Mosquitoes (Anopheles funestus) are vectors of malaria, and most strategies for combating the spread of the disease focus on control of mosquito populations using insecticides. The spread of resistance genes could hold back efforts to prevent the disease. The authors say that knowing how resistance works will help to develop tests, and stop these genes from spreading amongst mosquito populations.

Charles Wondji, senior author on the paper, from the Liverpool School of Tropical Medicine says: ‘We found a population of mosquitoes fully resistant to DDT (no mortality when they were treated with DDT) but also to pyrethroids. So we wanted to elucidate the molecular basis of that resistance in the population and design a field applicable diagnostic assay for its monitoring.’

They took mosquitoes from Pahou in Benin, which were resistant to DDT and pyrethroids, and mosquitoes from a laboratory fully susceptible strain and did a genome wide comparison study. They identified the GSTe2 gene as being upregulated - producing a lot of protein - in Benin mosquitoes.

The scientists found that a single mutation (L119F) changed a non-resistant version of the GSTe2 gene to a DDT resistant version. They designed a DNA-based diagnostic test for this type of resistance (metabolic resistance) and confirmed that this mutation was found in mosquitoes from other areas of the world with DDT resistance but was completely absent in regions without. X-ray crystallography of the protein coded by the gene illustrated exactly how the mutation conferred resistance, by opening up the ‘active site’ where DDT molecules bind to the protein, so more can be broken down. This means that the mosquito can survive by breaking down the poison into non-toxic substances.

They also introduced the gene into fruit flies (Drosophila melanogaster) and found they became resistant to DDT and pyrethroids compared to controls, confirming that just this single mutation is enough to make mosquitoes resistant to both DDT and permethrin.

Wondji says: ‘For the first time, we have been able to identify a molecular marker for metabolic resistance (the type of resistance most likely to lead to control failure) in a mosquito population and to design a DNA-based diagnostic assay. Such tools will allow control programs to detect and track resistance at an early stage in the field, which is an essential requirement to successfully tackle the growing problem of insecticide resistance in vector control. This significant progress opens the door for us to do this with other forms of resistance as well and in other vector species.’

-ENDS-

Media Contact
Anna Perman
Media Officer
BioMed Central
T: +44 (0)20 3192 2429
E: Anna.Perman@biomedcentral.com

Notes to Editor

1. A single mutation in the GSTe2 gene allows tracking of metabolically-based insecticide resistance in a major malaria vector
Riveron J M, Yunta C, Ibrahim S S, Djouaka R,Irving H, Menze B D, Ismail H M, Hemingway J, Ranson H, Albert A and Wondji C S
Genome Biology 2014, 15:R27
Article available at the journal website

Please name the journal in any story you write. If you are writing for the web, please link to the article. All articles are available free of charge, according to BioMed Central's open access policy.

2. Genome Biology publishes research articles, new methods and software tools, in addition to reviews and opinions, from the full spectrum of biology, including molecular, cellular, organism or population biology studied from a genomic perspective, as well as sequence analysis, bioinformatics, proteomics, comparative biology and evolution. @GenomeBiology
3. BioMed Central (http://www.biomedcentral.com/) is an STM (Science, Technology and Medicine) publisher which has pioneered the open access publishing model. All peer-reviewed research articles published by BioMed Central are made immediately and freely accessible online, and are licensed to allow redistribution and reuse. BioMed Central is part of Springer Science+Business Media, a leading global publisher in the STM sector. @BioMedCentral 

Advertisement