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BMC Proceedings publishes selected collections of research articles, conference proceedings, reviews and reports as supplements, which are free to access online. All articles published in supplements are subject to peer review; meeting abstracts undergo review and selection by the conference. Find out more about publishing a supplement with BioMed Central.

Volume 5 Supplement 8

22nd European Society for Animal Cell Technology (ESACT) Meeting on Cell Based Technologies

Meeting abstracts

22nd European Society for Animal Cell Technology (ESACT) Meeting on Cell Based Technologies

Vienna, Austria

15-18 May 2011

Edited by Hansjörg Hauser

Conference website

Introduction   Open Access

Cell Based Technologies: Abstracts of the 22nd ESACT Meeting 2011 in Vienna

Hansjörg Hauser BMC Proceedings 2011, 5(Suppl 8):I1 (22 November 2011)

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Meeting abstract   Open Access

Highly efficient, chemically defined and fully scalable biphasic production of vaccine viruses

Ingo Jordan, Volker Sandig BMC Proceedings 2011, 5(Suppl 8):O1 (22 November 2011)

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Meeting abstract   Open Access

Recombinant antibody mixtures; optimization of cell line generation and single-batch manufacturing processes

Søren K Rasmussen, Lars S Nielsen, Christian Müller, Thomas Bouquin, Henrik Næsted, Nina T Mønster, Frank Nygaard, Dietmar Weilguny, Torben P Frandsen, Anne B Tolstrup BMC Proceedings 2011, 5(Suppl 8):O2 (22 November 2011)

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Meeting abstract   Open Access

Cell-based medicinal products and the development of GMP-compliant processes and manufacturing

Luca Romagnoli, Ilaria Giuntini, Marta Galgano, Chiara Crosta, Luigi Cavenaghi, Maria Nolli BMC Proceedings 2011, 5(Suppl 8):O3 (22 November 2011)

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Meeting abstract   Open Access

Biosynthetic pathway deflection – a new cell line engineering approach

Hans Henning von Horsten, Thomas Rose, Volker Sandig BMC Proceedings 2011, 5(Suppl 8):O4 (22 November 2011)

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Meeting abstract   Open Access

Fluorescence-based tools to improve biopharmaceutical process development

Tiago M Duarte, Manuel JT Carrondo, Paula M Alves, Ana P Teixeira BMC Proceedings 2011, 5(Suppl 8):O5 (22 November 2011)

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Meeting abstract   Open Access

Towards rational engineering of cells: Recombinant gene expression in defined chromosomal loci

Kristina Nehlsen, Leonor da Gama-Norton, Roland Schucht, Hansjörg Hauser, Dagmar Wirth BMC Proceedings 2011, 5(Suppl 8):O6 (22 November 2011)

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Meeting abstract   Open Access

Human hair follicle equivalents in vitro for transplantation and chip-based substance testing

R Horland, G Lindner, I Wagner, B Atac, S Hoffmann, M Gruchow, F Sonntag, U Klotzbach, R Lauster, U Marx BMC Proceedings 2011, 5(Suppl 8):O7 (22 November 2011)

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Meeting abstract   Open Access

Chromosome identification and its application in Chinese hamster ovary cells

Yihua Cao, Shuichi Kimura, Joon-young Park, Miyuki Yamatani, Kohsuke Honda, Hisao Ohtake, Takeshi Omasa BMC Proceedings 2011, 5(Suppl 8):O8 (22 November 2011)

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Meeting abstract   Open Access

Development of an automated, multiwell plate based screening system for suspension cell culture

Sven Markert, Klaus Joeris BMC Proceedings 2011, 5(Suppl 8):O9 (22 November 2011)

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Meeting abstract   Open Access

Utilization of multifrequency permittivity measurements in addition to biomass monitoring

Christoph Heinrich, Tim Beckmann, Heino Büntemeyer, Thomas Noll BMC Proceedings 2011, 5(Suppl 8):O10 (22 November 2011)

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Meeting abstract   Open Access

ATF4 over-expression increased IgG1 productivity in Chinese hamster ovary cells

Ahmad M Haredy, Akitoshi Nishizawa, Kohsuke Honda, Tomoshi Ohya, Hisao Ohtake, Takeshi Omasa BMC Proceedings 2011, 5(Suppl 8):O11 (22 November 2011)

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Meeting abstract   Open Access

Characterization of novel pneumatic mixing for single-use bioreactor application

Brian Lee, David Fang, Matthew Croughan, Manuel Carrondo, Sang-Hoon Paik BMC Proceedings 2011, 5(Suppl 8):O12 (22 November 2011)

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Meeting abstract   Open Access

Enhanced protein synthesis and secretion using a rational signal-peptide library approach as a tailored tool

Beate Stern, Asta Optun, Melanie Liesenfeld, Christine Gey, Markus Gräfe, Ian F Pryme BMC Proceedings 2011, 5(Suppl 8):O13 (22 November 2011)

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Meeting abstract   Open Access

High-capacity assay to quantify the clonal heterogeneity in potency of mesenchymal stem cells

Kim C O’Connor, Katie C Russell, Donald G Phinney, Michelle R Lacey, Bonnie L Barrilleaux, Kristin E Meyertholen BMC Proceedings 2011, 5(Suppl 8):O14 (22 November 2011)

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Meeting abstract   Open Access

Improvement of cell freezing technologies: towards a fully closed process

Aurore Polès-Lahille, Brigitte Lafuente, Virginie Perrier, David Balbuena, Didier Peyret BMC Proceedings 2011, 5(Suppl 8):P1 (22 November 2011)

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Meeting abstract   Open Access

Disposable bioreactors: from process development to production

Aurore Polès-Lahille, Celine Richard, Sandrine Fisch, David Pedelaborde, Sandy Gerby, Nora Kadi, Virginie Perrier, Robert Trieau, David Balbuena, Laure Valognes, Didier Peyret BMC Proceedings 2011, 5(Suppl 8):P2 (22 November 2011)

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Meeting abstract   Open Access

Neuroprotective activity of a new erythropoietin formulation with increased penetration in the central nervous system

Marina Etcheverrigaray, Natalia Ceaglio, Mónica Mattio, Marcos Oggero, Ignacio Amadeo, Guillermina Forno, Norma Perotti, Ricardo Kratje BMC Proceedings 2011, 5(Suppl 8):P3 (22 November 2011)

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Meeting abstract   Open Access

New reporter cell clones to determine the biological activity of human type I interferons

Milagros Bürgi, Claudio Prieto, Marcos Oggero, Mariela Bollati-Fogolín, Marina Etcheverrigaray, Ricardo Kratje BMC Proceedings 2011, 5(Suppl 8):P4 (22 November 2011)

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Meeting abstract   Open Access

Analytical techniques for characterization of raw materials in cell culture media

Chandana Sharma, Barry Drew, Kevin Head, Rani Pusuluri, Matthew V Caple BMC Proceedings 2011, 5(Suppl 8):P5 (22 November 2011)

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Meeting abstract   Open Access

Next-generation sequencing of the CHO cell transcriptome

Jennifer Becker, Christina Timmermann, Tobias Jakobi, Oliver Rupp, Rafael Szczepanowski, Matthias Hackl, Alexander Goesmann, Andreas Tauch, Nicole Borth, Johannes Grillari, Alfred Pühler, Thomas Noll, Karina Brinkrolf BMC Proceedings 2011, 5(Suppl 8):P6 (22 November 2011)

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Meeting abstract   Open Access

A strategy to obtain recombinant cell lines with high expression levels. Lentiviral vector-mediated transgenesis

Claudio Prieto, Diego Fontana, Marina Etcheverrigaray, Ricardo Kratje BMC Proceedings 2011, 5(Suppl 8):P7 (22 November 2011)

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Meeting abstract   Open Access

In-situ cell density monitoring and apoptosis detection in adherent Vero cell bioreactor cultures

Emma Petiot, Amal El-Wajgali, Geoffrey Esteban, Cécile Gény, Hervé Pinton, Annie Marc BMC Proceedings 2011, 5(Suppl 8):P8 (22 November 2011)

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Meeting abstract   Open Access

Does earlier use of productivity enhancers during cell line selection lead to the identification of more productive cell lines?

Alison J Porter, Atul Mohindra, Juana Porter, Andrew J Racher BMC Proceedings 2011, 5(Suppl 8):P9 (22 November 2011)

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Meeting abstract   Open Access

Quantification of intracellular nucleotide sugars and formulation of a mathematical model for prediction of their metabolism

Ioscani Jiménez del Val, Judit M Nagy, Cleo Kontoravdi BMC Proceedings 2011, 5(Suppl 8):P10 (22 November 2011)

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Meeting abstract   Open Access

Design and simulation of a controller system for metabolic shift regulation in mammalian cells

Damián Baeza, Ziomara P Gerdtzen, Cristian J Salgado BMC Proceedings 2011, 5(Suppl 8):P11 (22 November 2011)

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Meeting abstract   Open Access

The way to a design space for an animal cell culture process according to Quality by Design (QbD)

Robert Puskeiler, Jan Kreuzmann, Caroline Schuster, Katharina Didzus, Nicole Bartsch, Christian Hakemeyer, Heike Schmidt, Melanie Jacobs, Stefan Wolf BMC Proceedings 2011, 5(Suppl 8):P12 (22 November 2011)

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Meeting abstract   Open Access

Improvement of stem cell performance by supplementation with metabolic enhancers

Abi M Abitorabi, Christopher Wilcox BMC Proceedings 2011, 5(Suppl 8):P13 (22 November 2011)

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Meeting abstract   Open Access

Use of Micro Bioreactor systems to streamline cell line evaluation and upstream process development for monoclonal antibody production

Steve R C Warr, Jai Patel, Rongzan Ho, Katy V Newell BMC Proceedings 2011, 5(Suppl 8):P14 (22 November 2011)

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Meeting abstract   Open Access

Cell line selection using the Duetz Microflask system

Steve R C Warr, Sharon L White, Yuen-Ting Chim, Jai Patel, Hella Bosteels BMC Proceedings 2011, 5(Suppl 8):P15 (22 November 2011)

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Meeting abstract   Open Access

Evaluation of an online biomass probe to monitor cell growth and cell death

Angelo Perani, Benjamin Gloria, Dongmao Wang, Anne-Sophie Buffier, Olivier Berteau, Geoffrey Esteban, Fiona E Smyth, Andrew M Scott BMC Proceedings 2011, 5(Suppl 8):P16 (22 November 2011)

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Meeting abstract   Open Access

Impact on product quality of high productive GS-CHO cell lines

Angelo Perani, Benjamin Gloria, Dongmao Wang, Roger Murphy, Harjit Singh, Fiona E Smyth, Andrew M Scott BMC Proceedings 2011, 5(Suppl 8):P17 (22 November 2011)

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Meeting abstract   Open Access

Anti-diabetes effect of water containing hydrogen molecule and Pt nanoparticles

Sanetaka Shirahata, Takeki Hamasaki, Keisuke Haramaki, Takuro Nakamura, Masumi Abe, Hanxu Yan, Tomoya Kinjo, Noboru Nakamichi, Shigeru Kabayama, Kiichiro Teruya BMC Proceedings 2011, 5(Suppl 8):P18 (22 November 2011)

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Meeting abstract   Open Access

Metabolic enhancers: a new paradigm in cell culture media optimization

Abi M Abitorabi, Christopher Wilcox BMC Proceedings 2011, 5(Suppl 8):P19 (22 November 2011)

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Meeting abstract   Open Access

Neuroprotective effects of 3,5-di-o-caffeoylquinic acid in vitro and in vivo

Junkyu Han, Hiroko Isoda BMC Proceedings 2011, 5(Suppl 8):P20 (22 November 2011)

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Meeting abstract   Open Access

Improvement of insulin resistance by Cyanidin 3-glucoside, anthocyanin from black beans through the up-regulation of GLUT4 gene expression

Tetsuya Inaguma, Junkyu Han, Hiroko Isoda BMC Proceedings 2011, 5(Suppl 8):P21 (22 November 2011)

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Meeting abstract   Open Access

Engineering CHO cell growth by stable manipulation of miRNA expression

Noëlia Sanchez, Nga Lao, Clair Gallagher, Martin Clynes, Niall Barron BMC Proceedings 2011, 5(Suppl 8):P22 (22 November 2011)

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Meeting abstract   Open Access

Expression of recombinant human coagulation factors VII (rFVII) and IX (rFIX) in various cell types, glycosylation analysis, and pharmacokinetic comparison

Ernst Böhm, Michael Dockal, Michael Graninger, Meinhard Hasslacher, Martin Kaliwoda, Christian Konetschny, Artur Mitterer, Eva-Maria Muchitsch, Manfred Reiter, Friedrich Scheiflinger BMC Proceedings 2011, 5(Suppl 8):P23 (22 November 2011)

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Meeting abstract   Open Access

Complex medium supplements optimized for the reduction of animal-derived components in vaccine production media

James F Babcock, Karen A Benedict, Amanda L Perlman BMC Proceedings 2011, 5(Suppl 8):P24 (22 November 2011)

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Meeting abstract   Open Access

Application of complex and chemically-defined medium supplements toward cell line specific performance enhancement of biopharmaceutical production systems

James F Babcock, Karen A Benedict, Amanda L Perlman BMC Proceedings 2011, 5(Suppl 8):P25 (22 November 2011)

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Meeting abstract   Open Access

A comparison of performance enhancing synergy among ultrafiltered yeast extracts and recombinant human serum albumin in CHO-K1 cells

James F Babcock, Karen A Benedict, Amanda L Perlman BMC Proceedings 2011, 5(Suppl 8):P26 (22 November 2011)

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Meeting abstract   Open Access

Targeted metabolomics for bioprocessing

Denise Sonntag, Francesca M Scandurra, Torben Friedrich, Michael Urban, Klaus M Weinberger BMC Proceedings 2011, 5(Suppl 8):P27 (22 November 2011)

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Meeting abstract   Open Access

Toolbox approach for fast generation of stable CHO production cell lines from different hosts

Susanne Seitz, Henning von Horsten, Thomas Rose, Volker Sandig, Karsten Winkler BMC Proceedings 2011, 5(Suppl 8):P28 (22 November 2011)

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Meeting abstract   Open Access

Growth characterization of CHO DP-12 cell lines with different high passage histories

Christoph Heinrich, Timo Wolf, Christina Kropp, Stefan Northoff, Thomas Noll BMC Proceedings 2011, 5(Suppl 8):P29 (22 November 2011)

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Meeting abstract   Open Access

Utilization of multifrequency permittivity measurements in addition to biomass monitoring

Christoph Heinrich, Tim Beckmann, Heino Büntemeyer, Thomas Noll BMC Proceedings 2011, 5(Suppl 8):P30 (22 November 2011)

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Meeting abstract   Open Access

CHO cell lines generated by PiggyBac transposition

Mattia Matasci, Virginie Bachmann, Lucia Baldi, David L Hacker, Maria De Jesus, Florian M Wurm BMC Proceedings 2011, 5(Suppl 8):P31 (22 November 2011)

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Meeting abstract   Open Access

Transposon mediated co-integration and co-expression of transgenes in CHO-DG44 cells

Sowmya Balasubramanian, Mattia Matasci, Lucia Baldi, David L Hacker, Florian M Wurm BMC Proceedings 2011, 5(Suppl 8):P32 (22 November 2011)

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Meeting abstract   Open Access

The use of filler DNA for improved transfection and reduced DNA needs in transient gene expression with CHO and HEK cells

Divor Kiseljak, Yashas Rajendra, Sagar S Manoli, Lucia Baldi, David L Hacker, Florian M Wurm BMC Proceedings 2011, 5(Suppl 8):P33 (22 November 2011)

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Meeting abstract   Open Access

Rapid recombinant protein production from pools of transposon-generated CHO cells

Mattia Matasci, Virginie Bachmann, Lucia Baldi, David L Hacker, Maria De Jesus, Florian M Wurm BMC Proceedings 2011, 5(Suppl 8):P34 (22 November 2011)

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Meeting abstract   Open Access

Influence of glutamine on transient and stable recombinant protein production in CHO and HEK-293 cells

Yashas Rajendra, Divor Kiseljak, Lucia Baldi, David L Hacker, Florian M Wurm BMC Proceedings 2011, 5(Suppl 8):P35 (22 November 2011)

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Meeting abstract   Open Access

kLa as a predictor for probe-independent mammalian cell bioprocesses in orbitally shaken bioreactors

Stéphanie Tissot, Dominique T Monteil, Lucia Baldi, David L Hacker, Florian M Wurm BMC Proceedings 2011, 5(Suppl 8):P36 (22 November 2011)

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Meeting abstract   Open Access

Transient transfection of insect Sf-9 cells in TubeSpin® bioreactor 50 tubes

Xiao Shen, Patrik O Michel, Qiuling Xie, David L Hacker, Florian M Wurm BMC Proceedings 2011, 5(Suppl 8):P37 (22 November 2011)

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Meeting abstract   Open Access

Transient gene expression with CHO cells in conditioned medium: a study using TubeSpin® bioreactors

João Pereira, Yashas Rajendra, Lucia Baldi, David L Hacker, Florian M Wurm BMC Proceedings 2011, 5(Suppl 8):P38 (22 November 2011)

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Meeting abstract   Open Access

Hydrodynamic stress in orbitally shaken bioreactors

Stéphanie Tissot, Martino Reclari, Samuel Quinodoz, Matthieu Dreyer, Dominique T Monteil, Lucia Baldi, David L Hacker, Mohamed Farhat, Marco Discacciati, Alfio Quarteroni, Florian M Wurm BMC Proceedings 2011, 5(Suppl 8):P39 (22 November 2011)

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Meeting abstract   Open Access

A platform fed-batch process for various CEMAX® producer cell lines

Benedikt Greulich, Marika Poppe, Henry Woischnig, Karlheinz Landauer, Andreas Herrmann BMC Proceedings 2011, 5(Suppl 8):P40 (22 November 2011)

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Meeting abstract   Open Access

Development of a chemically defined CHO medium by engineering based on a feed solution

Karlheinz Landauer, Henry Woischnig, Natalie Hepp, Benedikt Greulich, Andreas Herrmann BMC Proceedings 2011, 5(Suppl 8):P41 (22 November 2011)

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Meeting abstract   Open Access

Process development of ATROSAB, an anti TNFR1 Monoclonal Antibody: in three steps from research to GMP

Karlheinz Landauer, Cuneyt Unutmaz, Simon Egli, Verena Berger, Simone Lais, Timo Liebig, Daniel Steiner, Jo Maier, Ina Rostalski, Francois Forcellino, Andreas Herrmann BMC Proceedings 2011, 5(Suppl 8):P42 (22 November 2011)

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Meeting abstract   Open Access

Cell line development using the SEFEX system

Benedikt Greulich, Verena Berger, Marika Poppe, Natalie Hepp, Karlheinz Landauer, Andreas Herrmann BMC Proceedings 2011, 5(Suppl 8):P43 (22 November 2011)

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Meeting abstract   Open Access

Platform process for production of monoclonal antibodies for research purposes – improvement option

Joern Petersen, Claus Kristensen BMC Proceedings 2011, 5(Suppl 8):P44 (22 November 2011)

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Meeting abstract   Open Access

Development and validation of a protocol for cell line identification by MALDI-TOF MS

Guido Vogel, André Strauss, Bernard Jenni, Dominik Ziegler, Eric Dumermuth, Sylvie Antz, Claudia Bardouille, Beat Wipf, Christian Miscenic, Georg Schmid, Valentin Pflüger BMC Proceedings 2011, 5(Suppl 8):P45 (22 November 2011)

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Meeting abstract   Open Access

DoE of fed-batch processes – model-based design and experimental evaluation

Onur Sercinoglu, Oscar Platas Barradas, Volker Sandig, An-Ping Zeng, Ralf Pörtner BMC Proceedings 2011, 5(Suppl 8):P46 (22 November 2011)

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Meeting abstract   Open Access

Criteria for bioreactor comparison and operation standardisation during process development for mammalian cell culture

Oscar Platas Barradas, Uwe Jandt, Linh Da Minh Phan, Mario Villanueva, Alexander Rath, Udo Reichl, Eva Schräder, Sebastian Scholz, Thomas Noll, Volker Sandig, Ralf Pörtner, An-Ping Zeng BMC Proceedings 2011, 5(Suppl 8):P47 (22 November 2011)

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Meeting abstract   Open Access

Cultivation strategies of a BA/F3 cell line for fundamental cell research

Martin Schaletzky, Oscar Platas Barradas, Henning Sievert, Stefan Balabanov, An-Ping Zeng, Ralf Pörtner BMC Proceedings 2011, 5(Suppl 8):P48 (22 November 2011)

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Meeting abstract   Open Access

Physical methods for synchronization of a human production cell line

Oscar Platas Barradas, Uwe Jandt, Ralf Hass, Cornelia Kasper, Volker Sandig, Ralf Pörtner, An-Ping Zeng BMC Proceedings 2011, 5(Suppl 8):P49 (22 November 2011)

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Meeting abstract   Open Access

Efficient production of recombinant IgG by the GLUT5 co-expression system

Yuichi Inoue, Aiko Inoue, Hiroharu Kawahara BMC Proceedings 2011, 5(Suppl 8):P50 (22 November 2011)

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Meeting abstract   Open Access

Innovative animal component-free surface for the cultivation of human embryonic stem cells

Thomas Stelzer, Tina Marwood, Cindy Neeley BMC Proceedings 2011, 5(Suppl 8):P51 (22 November 2011)

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Meeting abstract   Open Access

Avian cell line - Technology for large scale vaccine production

Barbara Kraus, Simone von Fircks, Simone Feigl, Sabrina M Koch, Daniel Fleischanderl, Katherine Terler, Mitra Dersch-Pourmojib, Christian Konetschny, Leopold Grillberger, Manfred Reiter BMC Proceedings 2011, 5(Suppl 8):P52 (22 November 2011)

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Meeting abstract   Open Access

Towards human central nervous system in vitro models for preclinical research: strategies for 3D neural cell culture

Daniel Simão, Inês Costa, Margarida Serra, Johannes Schwarz, Catarina Brito, Paula M Alves BMC Proceedings 2011, 5(Suppl 8):P53 (22 November 2011)

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Meeting abstract   Open Access

Evaluation of a disposable stirred tank bioreactor for cultivation of mammalian cells

Alexander Hähnel, Benjamin Pütz, Kai Iding, Tabea Niediek, Frank Gudermann, Dirk Lütkemeyer BMC Proceedings 2011, 5(Suppl 8):P54 (22 November 2011)

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Meeting abstract   Open Access

Controlled expansion and differentiation of mesenchymal stem cells in a microcarrier based stirred bioreactor

Sébastien Sart, Abdelmounaim Errachid, Yves-Jacques Schneider, Spiros N Agathos BMC Proceedings 2011, 5(Suppl 8):P55 (22 November 2011)

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Meeting abstract   Open Access

3D6 and 4B3: Recombinant expression of two anti-gp41 antibodies as dimeric and secretory IgA

David Reinhart, Robert Weik, Renate Kunert BMC Proceedings 2011, 5(Suppl 8):P56 (22 November 2011)

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Meeting abstract   Open Access

Creating new opportunities in process control through radio frequency impedance spectroscopy

Daniel W Logan, John P Carvell, Matthew P H Lee BMC Proceedings 2011, 5(Suppl 8):P57 (22 November 2011)

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Meeting abstract   Open Access

Applications of nanoparticels in molecular and cellular biology and cancer research

Katya Simeonova, Ganka Milanova BMC Proceedings 2011, 5(Suppl 8):P58 (22 November 2011)

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Meeting abstract   Open Access

Measurement of sialic acid content on recombinant membrane proteins

Deniz Baycin-Hizal, Sunny Mai, Daniel Wolozny, Ilhan Akan, Noboru Tomiya, Karen Palter, Michael Betenbaugh BMC Proceedings 2011, 5(Suppl 8):P59 (22 November 2011)

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Meeting abstract   Open Access

Influence of operating parameters of a settling-based perfusion process on expansion of VERO cells attached on microcarriers

Amal El Wajgali, Frantz Fournier, Eric Olmos, Cécile Gény, Hervé Pinton, Annie Marc BMC Proceedings 2011, 5(Suppl 8):P60 (22 November 2011)

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Meeting abstract   Open Access

3D-Bioreactor culture of human hepatoma cell line HepG2 as a promising tool for in vitro substance testing

Christiane Goepfert, Wibke Scheurer, Susanne Rohn, Britta Rathjen, Stefanie Meyer, Anja Dittmann, Katharina Wiegandt, Rolf Janßen, Ralf Pörtner BMC Proceedings 2011, 5(Suppl 8):P61 (22 November 2011)

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Meeting abstract   Open Access

“BioProzessTrainer” as training tool for design of experiments

Ralf Pörtner, Oscar Platas-Barradas, Janosh Gradkowski, Richa Gautam, Florian Kuhnen, Volker C Hass BMC Proceedings 2011, 5(Suppl 8):P62 (22 November 2011)

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Meeting abstract   Open Access

ADCC potency assay: increased standardization with modified lymphocytes

Laurent Bretaudeau, Véronique Bonnaudet BMC Proceedings 2011, 5(Suppl 8):P63 (22 November 2011)

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Meeting abstract   Open Access

Anti-idiotypic antibody Ab2/3H6 mimicking gp41: a potential HIV-1 vaccine?

Renate Kunert, Alexander Mader BMC Proceedings 2011, 5(Suppl 8):P64 (22 November 2011)

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Meeting abstract   Open Access

Application of hydrocyclones for continuous cultivation of SP-2/0 cells in perfusion bioreactors: Effect of hydrocyclone operating pressure

Elsayed A Elsayed, Roland Wagner BMC Proceedings 2011, 5(Suppl 8):P65 (22 November 2011)

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Meeting abstract   Open Access

Improving volumetric productivity of a stable human CAP cell line by bioprocess optimization

Ruth Essers, Helmut Kewes, Gudrun Schiedner BMC Proceedings 2011, 5(Suppl 8):P66 (22 November 2011)

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Meeting abstract   Open Access

Development of a triculture based system for improved benefit/risk assessment in pharmacology and human food

Alexandra Bazes, Géraldine Nollevaux, Régis Coco, Aurélie Joly, Thérèse Sergent, Yves-Jacques Schneider BMC Proceedings 2011, 5(Suppl 8):P67 (22 November 2011)

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Meeting abstract   Open Access

Daphnane diterpene hirsein B downregulates melanogenesis in B16 murine melanoma cells by cAMP pathway inhibition

Myra O Villareal, Junkyu Han, Kenjiro Ikuta, Hiroko Isoda BMC Proceedings 2011, 5(Suppl 8):P68 (22 November 2011)

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Meeting abstract   Open Access

The neuroprotective effects of electrolyzed reduced water and its model water containing molecular hydrogen and Pt nanoparticles

Hanxu Yan, Taichi Kashiwaki, Takeki Hamasaki, Tomoya Kinjo, Kiichiro Teruya, Shigeru Kabayama, Sanetaka Shirahata BMC Proceedings 2011, 5(Suppl 8):P69 (22 November 2011)

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Meeting abstract   Open Access

Development and fine-tuning of a scale down model for process characterization studies of a monoclonal antibody upstream production process

Mareike Harmsen, Jimmy Stofferis, Laetitia Malphettes BMC Proceedings 2011, 5(Suppl 8):P70 (22 November 2011)

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Meeting abstract   Open Access

Insect cell lines and baculoviruses as effective biocontrol agents of forest pests

Guido F Caputo, Sardar S Sohi, Sharon V Hooey, Lawrence J Gringorten BMC Proceedings 2011, 5(Suppl 8):P71 (22 November 2011)

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Meeting abstract   Open Access

Down-regulation of CD81 in human cells producing HCV-E1/E2 retroVLPs

Ana F Rodrigues, Miguel R Guerreiro, Rute Castro, Hélio A Tomás, Charlotte Dalba, David Klatzmann, Paula M Alves, Manuel J T Carrondo, Ana S Coroadinha BMC Proceedings 2011, 5(Suppl 8):P72 (22 November 2011)

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Meeting abstract   Open Access

Evaluation of disposable filtration systems for harvesting high cell density fed batch processes

Antje Pegel, Friedemann Übele, Sven Reiser, Dethardt Müller, Gregor Dudziak BMC Proceedings 2011, 5(Suppl 8):P73 (22 November 2011)

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Meeting abstract   Open Access

Osteogenic Differentiation of adipose mesenchymal stem cells with BMP-2 embedded microspheres in a rotating bed bioreactor

Stefanie Boehm, Yael Lupu, Marcelle Machluf, Cornelia Kasper BMC Proceedings 2011, 5(Suppl 8):P74 (22 November 2011)

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Meeting abstract   Open Access

Medium and feed optimization for fed-batch production of a monoclonal antibody in CHO cells

Nadine Kochanowski, Gaetan Siriez, Sarah Roosens, Laetitia Malphettes BMC Proceedings 2011, 5(Suppl 8):P75 (22 November 2011)

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Meeting abstract   Open Access

In-situ microscopy and 2D fluorescence spectroscopy as online methods for monitoring CHO cells during cultivation

Sophia Bonk, Marko Sandor, Ferdinand Rüdinger, Bernd Tscheschke, Andreas Prediger, Alexander Babitzky, Dørte Solle, Sascha Beutel, Thomas Scheper BMC Proceedings 2011, 5(Suppl 8):P76 (22 November 2011)

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Meeting abstract   Open Access

On-line and real time cell counting and viability determination for animal cell process monitoring by in situ microscopy

Philipp Wiedemann, Markus Worf, Hans B Wiegemann, Florian Egner, Christian Schwiebert, Jeff Wilkesman, Jean S Guez, Juan C Quintana, Diego Assanza, Hajo Suhr BMC Proceedings 2011, 5(Suppl 8):P77 (22 November 2011)

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Meeting abstract   Open Access

Characterization of the human AGE1.HN cell line: a systems biology approach

Sebastian Scholz, Miriam Luebbecke, Alexander Rath, Eva Schraeder, Thomas Rose, Heino Büntemeyer, Thomas Scheper, Udo Reichl, Thomas Noll BMC Proceedings 2011, 5(Suppl 8):P78 (22 November 2011)

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Meeting abstract   Open Access

Influence of the nickel-titanium alloy components on biological functions

Akiko Ogawa, Ryota Akatsuka, Hidekazu Tamauchi, Katsuya Hio, Hideyuki Kanematsu BMC Proceedings 2011, 5(Suppl 8):P79 (22 November 2011)

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Meeting abstract   Open Access

Metabolomics - a useful tool for prediction of protein production and processing?

Jennifer Kronthaler, Timo Schmidberger, Christine Heel BMC Proceedings 2011, 5(Suppl 8):P80 (22 November 2011)

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Meeting abstract   Open Access

Accelerating process development through analysis of cell metabolism

Dirk Müller, Florian Kirchner, Stefanie Mangin, Klaus Mauch BMC Proceedings 2011, 5(Suppl 8):P81 (22 November 2011)

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Meeting abstract   Open Access

Evaluation of sampling and quenching procedures for the analysis of intracellular metabolites in CHO suspension cells

Judith Wahrheit, Jens Niklas, Elmar Heinzle BMC Proceedings 2011, 5(Suppl 8):P82 (22 November 2011)

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Meeting abstract   Open Access

Increasing productivity of hybridoma cell lines by sorting by side scattering light

Daniel Landgrebe, Cornelia Kasper, Thomas Scheper BMC Proceedings 2011, 5(Suppl 8):P83 (22 November 2011)

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Meeting abstract   Open Access

Compartmentation and channelling of metabolites in the human cell line AGE1.HN®

Jens Niklas, Volker Sandig, Elmar Heinzle BMC Proceedings 2011, 5(Suppl 8):P84 (22 November 2011)

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Meeting abstract   Open Access

Producer vs. parental cell – metabolic changes and burden upon α1-antitrypsin production in AGE1.HN®

Jens Niklas, Christian Priesnitz, Volker Sandig, Thomas Rose, Elmar Heinzle BMC Proceedings 2011, 5(Suppl 8):P85 (22 November 2011)

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Meeting abstract   Open Access

Analysis of the mitochondrial subproteome of the human cell line AGE1.HN – a contribution to a systems biology approach

Eva Schräder, Sebastian Scholz, Volker Sandig, Raimund Hoffrogge, Thomas Noll BMC Proceedings 2011, 5(Suppl 8):P86 (22 November 2011)

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Meeting abstract   Open Access

Characterisation of cultivation of the human cell line AGE1.HN.AAT

Eva Schräder, Sebastian Scholz, Jens Niklas, Alexander Rath, Oscar Barradas, Uwe Jandt, Volker Sandig, Thomas Rose, Ralf Pörtner, Udo Reichl, An-Ping Zeng, Elmar Heinzle, Thomas Noll BMC Proceedings 2011, 5(Suppl 8):P87 (22 November 2011)

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Meeting abstract   Open Access

Strategies in umbilical cord-derived mesenchymal stem cells expansion: influence of oxygen, culture medium and cell separation

Antonina Lavrentieva, Tim Hatlapatka, Ramona Winkler, Ralf Hass, Cornelia Kasper BMC Proceedings 2011, 5(Suppl 8):P88 (22 November 2011)

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Meeting abstract   Open Access

Abstract withdrawn

BMC Proceedings 2011, 5(Suppl 8):P89 (22 November 2011)

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Meeting abstract   Open Access

Abstract withdrawn

BMC Proceedings 2011, 5(Suppl 8):P90 (22 November 2011)

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Meeting abstract   Open Access

Discerning key parameters influencing high productivity and quality through recognition of patterns in process data

Huong Le, Marlene Castro-Melchor, Christian Hakemeyer, Christine Jung, Berthold Szperalski, George Karypis, Wei-Shou Hu BMC Proceedings 2011, 5(Suppl 8):P91 (22 November 2011)

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Meeting abstract   Open Access

Proteomic and metabolomic characterization of CHO DP-12 cell lines with different high passage histories

Tim Beckmann, Tobias Thüte, Christoph Heinrich, Heino Büntemeyer, Thomas Noll BMC Proceedings 2011, 5(Suppl 8):P92 (22 November 2011)

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Meeting abstract   Open Access

A method for metabolomic sampling of suspended animal cells using fast filtration

Martin Volmer, Julia Gettmann, Sebastian Scholz, Heino Büntemeyer, Thomas Noll BMC Proceedings 2011, 5(Suppl 8):P93 (22 November 2011)

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Meeting abstract   Open Access

Analysis of glycolytic flux as a rapid screen to identify low lactate producing CHO cell lines with desirable monoclonal antibody yield and glycan profile

Rachel Legmann, Julie Melito, Ilana Belzer, David Ferrick BMC Proceedings 2011, 5(Suppl 8):P94 (22 November 2011)

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Meeting abstract   Open Access

Influence of cell specific productivity on product quality

Ruchika Srivastava, Lavanya Rao, Kriti Shukla, Sunaina Prabhu, Saravanan Desan, Dinesh Baskar, Ankur Bhatnagar, Anuj Goel, Harish Iyer BMC Proceedings 2011, 5(Suppl 8):P95 (22 November 2011)

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Meeting abstract   Open Access

A novel peptide to enhance recombinant BMP-2 production in mammalian cell cultures

Aileen J Zhou, Cameron ML Clokie, Sean AF Peel BMC Proceedings 2011, 5(Suppl 8):P96 (22 November 2011)

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Meeting abstract   Open Access

Determination of intracellular antibody production, cell density, and viability of recombinant CHO-DG44 cells using the MACSQuant® Analyzer

Jörn Pluschke, Sandra Klausing, Annika Haseloff, Bernd Schröder, Tanno Hübel BMC Proceedings 2011, 5(Suppl 8):P97 (22 November 2011)

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Meeting abstract   Open Access

Bioreactor cultivation of CHO DP-12 cells under sodium butyrate treatment – comparative transcriptome analysis with CHO cDNA microarrays

Sandra Klausing, Oliver Krämer, Thomas Noll BMC Proceedings 2011, 5(Suppl 8):P98 (22 November 2011)

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Meeting abstract   Open Access

Characterization of chromatographic yeast extract fractions promoting CHO cell growth

Mathilde Mosser, Romain Kapel, Arnaud Aymes, Laurent-Michel Bonanno, Eric Olmos, Iris Besançon, Dominique Druaux, Isabelle Chevalot, Ivan Marc, Annie Marc BMC Proceedings 2011, 5(Suppl 8):P99 (22 November 2011)

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Meeting abstract   Open Access

Study of expansion of porcine bone marrow mesenchymal stem cells on microcarriers using various operating conditions

Caroline Ferrari, Frédérique Balandras, Emmanuel Guedon, Eric Olmos, Nguyen Tran, Isabelle Chevalot, Annie Marc BMC Proceedings 2011, 5(Suppl 8):P100 (22 November 2011)

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Meeting abstract   Open Access

Growth and death kinetics of CHO cells cultivated in continuous bioreactor at various agitation rates

Frédérique Balandras, Eric Olmos, Caroline Hecklau, Fabrice Blanchard, Emmanuel Guedon, Annie Marc BMC Proceedings 2011, 5(Suppl 8):P101 (22 November 2011)

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Meeting abstract   Open Access

Increasing antibody yield and modulating final product quality using the FreedomTM CHO-STM production platform

Michelle Sabourin, Ying Huang, Prasad Dhulipala, Shannon Beatty, Jian Liu, Peter Slade, Shawn Barrett, Shue-Yuan Wang, Karsten Winkler, Susanne Seitz, Thomas Rose, Volker Sandig, Peggy Lio, Steve Gorfien, Laurie Donahue-Hjelle, Graziella Piras BMC Proceedings 2011, 5(Suppl 8):P102 (22 November 2011)

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Meeting abstract   Open Access

Single use bioreactors for the clinical production of monoclonal antibodies – a study to analyze the performance of a CHO cell line and the quality of the produced monoclonal antibody

Sonja Diekmann, Constanze Dürr, Alexander Herrmann, Ingo Lindner, Daniela Jozic BMC Proceedings 2011, 5(Suppl 8):P103 (22 November 2011)

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Meeting abstract   Open Access

Development of live cultural pandemic influenza vaccine Vector-Flu

Elena A Nechaeva, Tatyana Y Sen’kina, Alexander B Ryzhikov, Irina F Radaeva, Ol’ga G P’yankova, Natal’ya V Danil’chenko, Tatyana M Sviridenko, Marina P Bogryantzeva, Natal’ya V Gilina, Nikolay A Varaksin, Tatyana G Ryabicheva, Irina V Kiseleva, Larisa G Rudenko BMC Proceedings 2011, 5(Suppl 8):P104 (22 November 2011)

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Meeting abstract   Open Access

Study of a recombinant CHO cell line producing a monoclonal antibody by ATF or TFF external filter perfusion in a WAVE Bioreactor™

Marie-Françoise Clincke, Carin Mölleryd, Ye Zhang, Eva Lindskog, Kieron Walsh, Véronique Chotteau BMC Proceedings 2011, 5(Suppl 8):P105 (22 November 2011)

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Meeting abstract   Open Access

Utilizing Roche Cedex Bio analyzer for in process monitoring in biotech production

Dörthe Druhmann, Sabrina Reinhard, Felizitas Schwarz, Christina Schaaf, Katrin Greisl, Tim Nötzel BMC Proceedings 2011, 5(Suppl 8):P106 (22 November 2011)

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Meeting abstract   Open Access

LDH-C can be differentially expressed during fermentation of CHO cells

Berthold Szperalski, Christine Jung, Zhixin Shao, Anne Kantardjieff, Wei-Shou Hu BMC Proceedings 2011, 5(Suppl 8):P107 (22 November 2011)

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Meeting abstract   Open Access

Stem cell: from basic theoretical assumptions and mathematical concepts to the computational models

Katya Simeonova, Ganka Milanova BMC Proceedings 2011, 5(Suppl 8):P108 (22 November 2011)

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Meeting abstract   Open Access

Comparison of the activity and pluripotency maintaining potential of human leukemia inhibitory factor (LIF) produced in E.coli and CHO cells

Claas Haake, Sophia Bonk, Jana Parsiegla, Magda Tomala, Komal Loya, Malte Sgodda, Tobias Cantz, Axel Schambach, Cornelia Kasper, Thomas Scheper BMC Proceedings 2011, 5(Suppl 8):P109 (22 November 2011)

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Meeting abstract   Open Access

Disulphide bond reduction of a therapeutic monoclonal antibody during cell culture manufacturing operations

Brian Mullan, Bryan Dravis, Amareth Lim, Ambrose Clarke, Susan Janes, Pete Lambooy, Don Olson, Tomas O’Riordan, Bruce Ricart, Alexander G Tulloch BMC Proceedings 2011, 5(Suppl 8):P110 (22 November 2011)

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Meeting abstract   Open Access

Preliminary evaluation of microcarrier culture for growth and monoclonal antibody production of CHO-K1 cells

M Rodrigues, Pedro Fernandes, A Costa, Mariana Henriques, Joana Azeredo, Rosário Oliveira BMC Proceedings 2011, 5(Suppl 8):P111 (22 November 2011)

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Meeting abstract   Open Access

Strategies for adaptation of mAb-producing CHO cells to serum-free medium

A Costa, M Rodrigues, Mariana Henriques, Rosário Oliveira, Joana Azeredo BMC Proceedings 2011, 5(Suppl 8):P112 (22 November 2011)

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Meeting abstract   Open Access

Impact of different influenza cultivation conditions on HA N-Glycosylation

Jana V Roedig, Erdmann Rapp, Yvonne Genzel, Udo Reichl BMC Proceedings 2011, 5(Suppl 8):P113 (22 November 2011)

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Meeting abstract   Open Access

Effect of iron sources on the glycosylation macroheterogeneity of human recombinant IFN-γ produced by CHO cells during batch processes

Marie-Françoise Clincke, Emmanuel Guedon, Frances T Yen, Virginie Ogier, Jean-Louis Goergen BMC Proceedings 2011, 5(Suppl 8):P114 (22 November 2011)

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Meeting abstract   Open Access

Characterization of metalloprotease and serine protease activities in batch CHO cell cultures: control of human recombinant IFN-γ proteolysis by addition of iron citrate

Marie-Françoise Clincke, Emmanuel Guedon, Frances T Yen, Virginie Ogier, Jean-Louis Goergen BMC Proceedings 2011, 5(Suppl 8):P115 (22 November 2011)

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Meeting abstract   Open Access

Isolation of active peptides from plant hydrolysates that promote Vero cells growth in stirred cultures

Samia Rourou, Rym Hssiki, Héla Kallel BMC Proceedings 2011, 5(Suppl 8):P116 (22 November 2011)

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Meeting abstract   Open Access

Comparison of different membrane supports for monolayer culture of bovine oviduct epithelial cells

Muhammad Z Tahir, Fabien George, Isabelle Donnay BMC Proceedings 2011, 5(Suppl 8):P117 (22 November 2011)

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Meeting abstract   Open Access

Efficacy of an inactivated and adjuvanted “ZULVAC® 8 OVIS” vaccine produced using single-use bioreactors

Lídia Garcia, Helena Paradell, Mercedes Mouriño, Berta Alberca, Alicia Urniza, Ana Vila, Margarita Tarrats, Joan Plana-Durán BMC Proceedings 2011, 5(Suppl 8):P118 (22 November 2011)

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Meeting abstract   Open Access

Engineering CHO cell metabolism for growth in galactose

Natalia E Jiménez, Camila A Wilkens, Ziomara P Gerdtzen BMC Proceedings 2011, 5(Suppl 8):P119 (22 November 2011)

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Meeting abstract   Open Access

Engineering CHO cells for improved central carbon and energy metabolism

Camila A Wilkens, Ziomara P Gerdtzen BMC Proceedings 2011, 5(Suppl 8):P120 (22 November 2011)

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Meeting abstract   Open Access

Mitogenic effect of sericin on mammalian cells

Wataru Sato, Ken Fukumoto, Kana Yanagihara, Masahiro Sasaki, Yoshihiro Kunitomi, Satoshi Terada BMC Proceedings 2011, 5(Suppl 8):P121 (22 November 2011)

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Meeting abstract   Open Access

Challenges in scaling up a perfusion process

Vana Raja, Saravanan Desan, Ankur Bhatnagar, Anuj Goel, Harish Iyer BMC Proceedings 2011, 5(Suppl 8):P122 (22 November 2011)

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Meeting abstract   Open Access

High cell density growth of High Five suspension cells in DO-controlled wave-mixed bioreactors

Teddy Beltrametti, Nicole C Bögli, Gerhard Greller, Regine Eibl, Dieter Eibl BMC Proceedings 2011, 5(Suppl 8):P123 (22 November 2011)

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Meeting abstract   Open Access

Bag-based rapid and safe seed-train expansion method for Trichoplusia ni suspension cells

Nicole C Bögli, Christoph Ries, Irina Bauer, Thorsten Adams, Gerhard Greller, Regine Eibl, Dieter Eibl BMC Proceedings 2011, 5(Suppl 8):P124 (22 November 2011)

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Meeting abstract   Open Access

On-line monitoring of the live cell concentration in bioreactors based on a rocking platform

John Carvell, Matt Lee BMC Proceedings 2011, 5(Suppl 8):P125 (22 November 2011)

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Meeting abstract   Open Access

Optimization of HEK 293 cell growth by addition of non-animal derived components using design of experiments

Laura Cervera, Sonia Gutiérrez, Francesc Gòdia, María M Segura BMC Proceedings 2011, 5(Suppl 8):P126 (22 November 2011)

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Meeting abstract   Open Access

Evaluation of three commercial kits for mycoplasma NAT assays: selection and quality improvement

Fabien Dorange, Frédérick Le Goff, Nicolas Dumey BMC Proceedings 2011, 5(Suppl 8):P127 (22 November 2011)

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Meeting abstract   Open Access

Large pilot scale cultivation process study of adherent MDBK cells for porcine Influenza A virus propagation using a novel disposable stirred-tank bioreactor

Boris Hundt, Noreen Mölle, Sabine Stefaniak, Ralf Dürrwald, Joerg Weyand BMC Proceedings 2011, 5(Suppl 8):P128 (22 November 2011)

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Meeting abstract   Open Access

Effect of influenza virus infection on key metabolic enzyme activities in MDCK cells

Robert Janke, Yvonne Genzel, Maria Wetzel, Udo Reichl BMC Proceedings 2011, 5(Suppl 8):P129 (22 November 2011)

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Meeting abstract   Open Access

Novel human partner cell line for immortalisation of rare antigen-specific B cells in mAb development

Galina Kaseko, Marjorie Liu, Qiong Li, Tohsak Mahaworasilpa BMC Proceedings 2011, 5(Suppl 8):P130 (22 November 2011)

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Meeting abstract   Open Access

Application of the novel and convenient IR/MAR gene amplification technology to the production of recombinant protein pharmaceuticals

Yoshio Araki, Chiemi Noguchi, Tetsuro Hamafuji, Hiroshi Nose, Daisuke Miki, Noriaki Shimizu BMC Proceedings 2011, 5(Suppl 8):P131 (22 November 2011)

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Meeting abstract   Open Access

About making a CHO production cell line “research-friendly” by genetic engineering

Bernd Voedisch, Agnès Patoux, Jildou Sterkenburgh, Mirjam Buchs, Emily Barry, Cyril Allard, Sabine Geisse BMC Proceedings 2011, 5(Suppl 8):P132 (22 November 2011)

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Meeting abstract   Open Access

CAP-T cell expression system: a novel rapid and versatile human cell expression system for fast and high yield transient protein expression

Jens Wölfel, Ruth Essers, Corinna Bialek, Sabine Hertel, Nadine Scholz-Neumann, Gudrun Schiedner BMC Proceedings 2011, 5(Suppl 8):P133 (22 November 2011)

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Meeting abstract   Open Access

Production and purification of TGFb-1 in CHO-Cells

Estabraq Abdulkerim, Sabrina Baganz, Axel Schambach, Cornelia Kasper, Thomas Scheper BMC Proceedings 2011, 5(Suppl 8):P134 (20 December 2011)

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