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	1. 1258 Accesses	Methodology article The RIN: an RNA integrity number for assigning integrity values to RNA measurements Andreas Schroeder, Odilo Mueller, Susanne Stocker, Ruediger Salowsky, Michael Leiber, Marcus Gassmann, Samar Lightfoot, Wolfram Menzel, Martin Granzow, Thomas Ragg BMC Molecular Biology 2006, 7:3 (31 January 2006) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	2. 1098 Accesses	Research article The Drosophila methyl-DNA binding protein MBD2/3 interacts with the NuRD complex via p55 and MI-2 Joachim Marhold, Alexander Brehm, Katja Kramer BMC Molecular Biology 2004, 5:20 (29 October 2004) Abstract \| Full text \| PDF \| PubMed
	3. 985 Accesses	Review Human single-stranded DNA binding proteins are essential for maintaining genomic stability Nicholas W Ashton, Emma Bolderson, Liza Cubeddu, Kenneth J O’Byrne, Derek J Richard BMC Molecular Biology 2013, 14:9 (1 April 2013) Abstract \| Full text \| PDF \| ePUB \| PubMed
	4. 492 Accesses	Reviewer acknowledgement Annual acknowledgement of manuscript reviewers Christopher Morrey BMC Molecular Biology 2013, 14:8 (28 March 2013) Abstract \| Full text \| PDF \| ePUB \| PubMed
	5. 469 Accesses	Research article Identification of differences in microRNA transcriptomes between porcine oxidative and glycolytic skeletal muscles Yingkai Liu, Mingzhou Li, Jideng Ma, Jie Zhang, Chaowei Zhou, Tao Wang, Xiaolian Gao, Xuewei Li BMC Molecular Biology 2013, 14:7 (18 February 2013) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Analysis of miRNA expression profiling between the porcine longissimus doris muscle and psoas major muscle using a deep sequencing approach suggests that miRNAs play essential roles in the phenotypic variations observed in these muscle fiber types.
	6. 439 Accesses	Research article Validation of reference genes for quantitative expression analysis by real-time RT-PCR in Saccharomyces cerevisiae Marie-Ange Teste, Manon Duquenne, Jean M François, Jean-Luc Parrou BMC Molecular Biology 2009, 10:99 (30 October 2009) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	7. 435 Accesses	Methodology article Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR Nicholas Silver, Steve Best, Jie Jiang, Swee Thein BMC Molecular Biology 2006, 7:33 (6 October 2006) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	8. 435 Accesses	Research article Relative quantification of mRNA: comparison of methods currently used for real-time PCR data analysis Štefan Čikoš, Alexandra Bukovská, Juraj Koppel BMC Molecular Biology 2007, 8:113 (20 December 2007) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	9. 414 Accesses	Research article Validation of housekeeping genes for quantitative real-time PCR in in-vivo and in-vitro models of cerebral ischaemia Carme Gubern, Olivia Hurtado, Rocío Rodríguez, Jesús R Morales, Víctor G Romera, María A Moro, Ignacio Lizasoain, Joaquín Serena, Judith Mallolas BMC Molecular Biology 2009, 10:57 (16 June 2009) Abstract \| Full text \| PDF \| PubMed
	10. 415 Accesses	Review Cruciform structures are a common DNA feature important for regulating biological processes Václav Brázda, Rob C Laister, Eva B Jagelská, Cheryl Arrowsmith BMC Molecular Biology 2011, 12:33 (5 August 2011) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Cheryl Arrowsmith and colleagues review the role of cruciform structures in the regulation of major biological processes, including DNA replication, transcription, repair, recombination, and chromatin remodeling.
	11. 411 Accesses	Editorial MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments Stephen A Bustin, Jean-François Beaulieu, Jim Huggett, Rolf Jaggi, Frederick SB Kibenge, Pål A Olsvik, Louis C Penning, Stefan Toegel BMC Molecular Biology 2010, 11:74 (21 September 2010) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| 1 comment \| Editor’s summary The Editorial Board of BMC Molecular Biology endorse a new set of essential MIQE-light guidelines for the reporting of quantitative PCR data: "MIQE precis", and provide guidance for the suitability of pure reference gene papers to the journal.
	12. 406 Accesses	Methodology article Use of the lambda Red recombinase system to rapidly generate mutants in Pseudomonas aeruginosa Biliana Lesic, Laurence G Rahme BMC Molecular Biology 2008, 9:20 (4 February 2008) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	13. 391 Accesses	Methodology article Selection of suitable housekeeping genes for expression analysis in glioblastoma using quantitative RT-PCR Valeria Valente, Silvia A Teixeira, Luciano Neder, Oswaldo K Okamoto, Sueli M Oba-Shinjo, Suely KN Marie, Carlos A Scrideli, Maria L Paçó-Larson, Carlos G Carlotti BMC Molecular Biology 2009, 10:17 (3 March 2009) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	14. 387 Accesses	Research article Fork head transcription factor is required for ovarian mature in the brown planthopper, Nilaparvata lugens (Stål) Xiaolin Dong, Yifan Zhai, Jianqing Zhang, Zhongxiang Sun, Jing Chen, Jie Chen, Wenqing Zhang BMC Molecular Biology 2011, 12:53 (31 December 2011) Abstract \| Full text \| PDF \| PubMed
	15. 368 Accesses	Research article Characterization of housekeeping genes in zebrafish: male-female differences and effects of tissue type, developmental stage and chemical treatment Amy T McCurley, Gloria V Callard BMC Molecular Biology 2008, 9:102 (12 November 2008) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	16. 362 Accesses	Research article In search of suitable reference genes for gene expression studies of human renal cell carcinoma by real-time PCR Monika Jung, Azizbek Ramankulov, Jan Roigas, Manfred Johannsen, Martin Ringsdorf, Glen Kristiansen, Klaus Jung BMC Molecular Biology 2007, 8:47 (8 June 2007) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	17. 338 Accesses	Methodology article Detection limits of several commercial reverse transcriptase enzymes: impact on the low- and high-abundance transcript levels assessed by quantitative RT-PCR Jean-Philippe Levesque-Sergerie, Mathieu Duquette, Catherine Thibault, Louis Delbecchi, Nathalie Bissonnette BMC Molecular Biology 2007, 8:93 (22 October 2007) Abstract \| Full text \| PDF \| PubMed
	18. 338 Accesses	Methodology article Selection of reference genes for gene expression studies in pig tissues using SYBR green qPCR Ann-Britt Nygard, Claus B Jørgensen, Susanna Cirera, Merete Fredholm BMC Molecular Biology 2007, 8:67 (15 August 2007) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	19. 333 Accesses	Methodology article Single cell transcriptomic analysis of prostate cancer cells Christopher J Welty, Ilsa Coleman, Roger Coleman, Bryce Lakely, Jing Xia, Shu Chen, Roman Gulati, Sandy R Larson, Paul H Lange, Bruce Montgomery, Peter S Nelson, Robert L Vessella, Colm Morrissey BMC Molecular Biology 2013, 14:6 (16 February 2013) Abstract \| Full text \| PDF \| PubMed
	20. 319 Accesses	Methodology article Standardized collection of MNase-seq experiments enables unbiased dataset comparisons Jason M Rizzo, Jonathan E Bard, Michael J Buck BMC Molecular Biology 2012, 13:15 (6 May 2012) Abstract \| Full text \| PDF \| PubMed
	21. 306 Accesses	Research article Identification and validation of reference genes for quantitative RT-PCR normalization in wheat Anna R Paolacci, Oronzo A Tanzarella, Enrico Porceddu, Mario Ciaffi BMC Molecular Biology 2009, 10:11 (20 February 2009) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	22. 286 Accesses	Research article Effect of experimental treatment on GAPDH mRNA expression as a housekeeping gene in human diploid fibroblasts Azalina Zainuddin, Kien Chua, Norhazira Rahim, Suzana Makpol BMC Molecular Biology 2010, 11:59 (14 August 2010) Abstract \| Full text \| PDF \| PubMed
	23. 284 Accesses	Research article Selection of reliable reference genes for gene expression studies in peach using real-time PCR Zhaoguo Tong, Zhihong Gao, Fei Wang, Jun Zhou, Zhen Zhang BMC Molecular Biology 2009, 10:71 (20 July 2009) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	24. 282 Accesses	Methodology article RNA extraction from ten year old formalin-fixed paraffin-embedded breast cancer samples: a comparison of column purification and magnetic bead-based technologies Alfredo Ribeiro-Silva, Haiyu Zhang, Stefanie S Jeffrey BMC Molecular Biology 2007, 8:118 (21 December 2007) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	25. 266 Accesses	Methodology article Identification of suitable endogenous control genes for microRNA gene expression analysis in human breast cancer Pamela A Davoren, Roisin E McNeill, Aoife J Lowery, Michael J Kerin, Nicola Miller BMC Molecular Biology 2008, 9:76 (21 August 2008) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	26. 249 Accesses	Research article Evaluation of putative reference genes for gene expression normalization in soybean by quantitative real-time RT-PCR Ruibo Hu, Chengming Fan, Hongyu Li, Qingzhu Zhang, Yong-Fu Fu BMC Molecular Biology 2009, 10:93 (28 September 2009) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	27. 245 Accesses	Methodology article The Renilla luciferase gene as a reference gene for normalization of gene expression in transiently transfected cells Meesbah Jiwaji, Rónán Daly, Kshama Pansare, Pauline McLean, Jingli Yang, Walter Kolch, Andrew R Pitt BMC Molecular Biology 2010, 11:103 (31 December 2010) Abstract \| Full text \| PDF \| PubMed
	28. 245 Accesses	Research article Screening and analysis of PoAkirin1 and two related genes in response to immunological stimulants in the Japanese flounder (Paralichthys olivaceus) Chang-Geng Yang, Xian-Li Wang, Bo Zhang, Bing Sun, Shan-Shan Liu, Song-Lin Chen BMC Molecular Biology 2013, 14:10 (7 May 2013) Abstract \| Provisional PDF \| PubMed
	29. 244 Accesses	Methodology article Use of the lambda Red recombinase system to produce recombinant prophages carrying antibiotic resistance genes Ruth Serra-Moreno, Sandra Acosta, Jean Hernalsteens, Juan Jofre, Maite Muniesa BMC Molecular Biology 2006, 7:31 (19 September 2006) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	30. 243 Accesses	Research article Multiple tandem splicing silencer elements suppress aberrant splicing within the long exon 26 of the human Apolipoprotein B gene Umasuthan Srirangalingam, Scott A Akker, Dennis Norman, Naveenan Navaratnam, Shern L Chew, Bernard Khoo BMC Molecular Biology 2013, 14:5 (7 February 2013) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Pseudo splice sites are heterogeneously distributed along exon 26 of Apolipoprotein B and are repressed by multiple, tandem splicing silencers which may be key to maintaining integrity during RNA splicing and to the expression of APOB isoforms.
	31. 241 Accesses	Research article Validation of reference genes for gene expression analysis in chicory (Cichorium intybus) using quantitative real-time PCR Asad Maroufi, Erik Van Bockstaele, Marc De Loose BMC Molecular Biology 2010, 11:15 (15 February 2010) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	32. 230 Accesses	Research article Validation of internal control for gene expression study in soybean by quantitative real-time PCR Bo Jian, Bin Liu, Yurong Bi, Wensheng Hou, Cunxiang Wu, Tianfu Han BMC Molecular Biology 2008, 9:59 (23 June 2008) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	33. 228 Accesses	Research article Identification of suitable internal control genes for expression studies in Coffea arabica under different experimental conditions Carla F Barsalobres-Cavallari, Fábio E Severino, Mirian P Maluf, Ivan G Maia BMC Molecular Biology 2009, 10:1 (6 January 2009) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	34. 228 Accesses	Methodology article Evaluation of real-time PCR endogenous control genes for analysis of gene expression in bovine endometrium Caroline G Walker, Susanne Meier, Murray D Mitchell, John R Roche, Mathew Littlejohn BMC Molecular Biology 2009, 10:100 (1 November 2009) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	35. 226 Accesses	Research article Comparing protocols for preparation of DNA-free total yeast RNA suitable for RT-PCR Eduardo M Del Aguila, Marcio B Dutra, Joab T Silva, Vânia MF Paschoalin BMC Molecular Biology 2005, 6:9 (15 April 2005) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	36. 222 Accesses	Research article Off-target effects of siRNA specific for GFP Cordula Tschuch, Angela Schulz, Armin Pscherer, Wiebke Werft, Axel Benner, Agnes Hotz-Wagenblatt, Leticia Barrionuevo, Peter Lichter, Daniel Mertens BMC Molecular Biology 2008, 9:60 (24 June 2008) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	37. 218 Accesses	Methodology article Efficient four fragment cloning for the construction of vectors for targeted gene replacement in filamentous fungi Rasmus JN Frandsen, Jens A Andersson, Matilde B Kristensen, Henriette Giese BMC Molecular Biology 2008, 9:70 (1 August 2008) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	38. 216 Accesses	Methodology article Analysis of current and alternative phenol based RNA extraction methodologies for cyanobacteria Fernando Pinto, Anders Thapper, Wolfgang Sontheim, Peter Lindblad BMC Molecular Biology 2009, 10:79 (7 August 2009) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	39. 213 Accesses	Research article The four hexamerin genes in the honey bee: structure, molecular evolution and function deduced from expression patterns in queens, workers and drones Juliana R Martins, Francis MF Nunes, Alexandre S Cristino, Zilá LP Simões, Márcia MG Bitondi BMC Molecular Biology 2010, 11:23 (26 March 2010) Abstract \| Full text \| PDF \| PubMed
	40. 207 Accesses	Methodology article Preamplification techniques for real-time RT-PCR analyses of endomyocardial biopsies Michel Noutsias, Maria Rohde, Andrea Block, Katrin Klippert, Olga Lettau, Katja Blunert, Michael Hummel, Uwe Kühl, Hans Lehmkuhl, Roland Hetzer, Ursula Rauch, Wolfgang Poller, Matthias Pauschinger, Heinz P Schultheiss, Hans D Volk, Katja Kotsch BMC Molecular Biology 2008, 9:3 (14 January 2008) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	41. 206 Accesses	Research article Instability of standard PCR reference genes in adipose-derived stem cells during propagation, differentiation and hypoxic exposure Trine Fink, Pia Lund, Linda Pilgaard, Jeppe Rasmussen, Meg Duroux, Vladimir Zachar BMC Molecular Biology 2008, 9:98 (31 October 2008) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	42. 202 Accesses	Research article Association of ATRX with pericentric heterochromatin and the Y chromosome of neonatal mouse spermatogonia Claudia Baumann, Anja Schmidtmann, Kathrin Muegge, Rabindranath De La Fuente BMC Molecular Biology 2008, 9:29 (13 March 2008) Abstract \| Full text \| PDF \| PubMed
	43. 202 Accesses	Research article Identification of a set of miRNAs differentially expressed in transiently TIA-depleted HeLa cells by genome-wide profiling Carmen Sánchez-Jiménez, Isabel Carrascoso, Juan Barrero, José M Izquierdo BMC Molecular Biology 2013, 14:4 (6 February 2013) Abstract \| Full text \| PDF \| PubMed
	44. 197 Accesses	Research article Alternative splicing of the neurofibromatosis type 1 pre-mRNA is regulated by the muscleblind-like proteins and the CUG-BP and ELAV-like factors Victoria A Fleming, Cuiyu Geng, Andrea N Ladd, Hua Lou BMC Molecular Biology 2012, 13:35 (10 December 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Muscleblind-like (MBNL) splicing proteins MBNL1 and MBNL2 positively regulate the inclusion of neurofibromatosis type 1 (NF1) exon 23a, and neighboring sequences together with the preferred binding sequence are required for optimal binding in vitro.
	45. 190 Accesses	Research article Distinctive expression patterns of 185/333 genes in the purple sea urchin, Strongylocentrotus purpuratus: an unexpectedly diverse family of transcripts in response to LPS, β-1,3-glucan, and dsRNA David P Terwilliger, Katherine M Buckley, Virginia Brockton, Nicole J Ritter, L Courtney Smith BMC Molecular Biology 2007, 8:16 (1 March 2007) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	46. 188 Accesses	Methodology article From Gateway to MultiSite Gateway in one recombination event Enrico Magnani, Linnea Bartling, Sarah Hake BMC Molecular Biology 2006, 7:46 (6 December 2006) Abstract \| Full text \| PDF \| PubMed
	47. 183 Accesses	Research article Single-stranded heteroduplex intermediates in λ Red homologous recombination Marcello Maresca, Axel Erler, Jun Fu, Anne Friedrich, Youming Zhang, A Francis Stewart BMC Molecular Biology 2010, 11:54 (29 July 2010) Abstract \| Full text \| PDF \| PubMed
	48. 182 Accesses	Research article Expression profiling and cross-species RNA interference (RNAi) of desiccation-induced transcripts in the anhydrobiotic nematode Aphelenchus avenae Wesley Reardon, Sohini Chakrabortee, Tiago Pereira, Trevor Tyson, Matthew C Banton, Katharine M Dolan, Bridget A Culleton, Michael J Wise, Ann M Burnell, Alan Tunnacliffe BMC Molecular Biology 2010, 11:6 (19 January 2010) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary In response to falling humidity, the nematode Aphelenchus avenae upregulates a set of genes before entering a state of suspended animation, upregulated glutathione peroxidase making a demonstrable contribution to surviving extreme dessication.
	49. 182 Accesses	Research article Evaluation of reference genes for real-time quantitative PCR studies in Candida glabrata following azole treatment Qingdi Li, Jeff Skinner, John E Bennett BMC Molecular Biology 2012, 13:22 (29 June 2012) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	50. 181 Accesses	Research article Translation initiation region sequence preferences in Escherichia coli Vladimir Vimberg, Age Tats, Maido Remm, Tanel Tenson BMC Molecular Biology 2007, 8:100 (31 October 2007) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central