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1.

1065
Accesses

Research article   Open Access Highly Accessed

The Drosophila methyl-DNA binding protein MBD2/3 interacts with the NuRD complex via p55 and MI-2

Joachim Marhold, Alexander Brehm, Katja Kramer BMC Molecular Biology 2004, 5:20 (29 October 2004)

Abstract | Full text | PDF | PubMed

2.

1054
Accesses

Methodology article   Open Access Highly Accessed

The RIN: an RNA integrity number for assigning integrity values to RNA measurements

Andreas Schroeder, Odilo Mueller, Susanne Stocker, Ruediger Salowsky, Michael Leiber, Marcus Gassmann, Samar Lightfoot, Wolfram Menzel, Martin Granzow, Thomas Ragg BMC Molecular Biology 2006, 7:3 (31 January 2006)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

3.

659
Accesses

Research article   Open Access

Characterization of Stra8 in Southern catfish (Silurus meridionalis): evidence for its role in meiotic initiation

Ranran Dong, Shijie Yang, Jing Jiao, Tingru Wang, Hongjuan Shi, Linyan Zhou, Yaoguang Zhang, Deshou Wang BMC Molecular Biology 2013, 14:11 (22 May 2013)

Abstract | Full text | PDF | ePUB | PubMed

4.

651
Accesses

Review   Open Access Highly Accessed

Human single-stranded DNA binding proteins are essential for maintaining genomic stability

Nicholas W Ashton, Emma Bolderson, Liza Cubeddu, Kenneth J O’Byrne, Derek J Richard BMC Molecular Biology 2013, 14:9 (1 April 2013)

Abstract | Full text | PDF | ePUB | PubMed

5.

461
Accesses

Methodology article   Open Access Highly Accessed

Selection of suitable housekeeping genes for expression analysis in glioblastoma using quantitative RT-PCR

Valeria Valente, Silvia A Teixeira, Luciano Neder, Oswaldo K Okamoto, Sueli M Oba-Shinjo, Suely KN Marie, Carlos A Scrideli, Maria L Paçó-Larson, Carlos G Carlotti BMC Molecular Biology 2009, 10:17 (3 March 2009)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

6.

439
Accesses

Methodology article   Open Access Highly Accessed

Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR

Nicholas Silver, Steve Best, Jie Jiang, Swee Thein BMC Molecular Biology 2006, 7:33 (6 October 2006)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

7.

440
Accesses

Research article   Open Access Highly Accessed

Characterization of housekeeping genes in zebrafish: male-female differences and effects of tissue type, developmental stage and chemical treatment

Amy T McCurley, Gloria V Callard BMC Molecular Biology 2008, 9:102 (12 November 2008)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

8.

386
Accesses

Research article   Open Access Highly Accessed

Validation of housekeeping genes for quantitative real-time PCR in in-vivo and in-vitro models of cerebral ischaemia

Carme Gubern, Olivia Hurtado, Rocío Rodríguez, Jesús R Morales, Víctor G Romera, María A Moro, Ignacio Lizasoain, Joaquín Serena, Judith Mallolas BMC Molecular Biology 2009, 10:57 (16 June 2009)

Abstract | Full text | PDF | PubMed

9.

378
Accesses

Research article   Open Access Highly Accessed

Validation of reference genes for quantitative expression analysis by real-time RT-PCR in Saccharomyces cerevisiae

Marie-Ange Teste, Manon Duquenne, Jean M François, Jean-Luc Parrou BMC Molecular Biology 2009, 10:99 (30 October 2009)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

10.

367
Accesses

Research article   Open Access Highly Accessed

In search of suitable reference genes for gene expression studies of human renal cell carcinoma by real-time PCR

Monika Jung, Azizbek Ramankulov, Jan Roigas, Manfred Johannsen, Martin Ringsdorf, Glen Kristiansen, Klaus Jung BMC Molecular Biology 2007, 8:47 (8 June 2007)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

11.

357
Accesses

Review   Open Access Highly Accessed

Cruciform structures are a common DNA feature important for regulating biological processes

Václav Brázda, Rob C Laister, Eva B Jagelská, Cheryl Arrowsmith BMC Molecular Biology 2011, 12:33 (5 August 2011)

Abstract | Full text | PDF | PubMed |  Editor’s summary

Cheryl Arrowsmith and colleagues review the role of cruciform structures in the regulation of major biological processes, including DNA replication, transcription, repair, recombination, and chromatin remodeling.

12.

352
Accesses

Research article   Open Access Highly Accessed

Relative quantification of mRNA: comparison of methods currently used for real-time PCR data analysis

Štefan Čikoš, Alexandra Bukovská, Juraj Koppel BMC Molecular Biology 2007, 8:113 (20 December 2007)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

13.

349
Accesses

Methodology article   Open Access Highly Accessed

Use of the lambda Red recombinase system to rapidly generate mutants in Pseudomonas aeruginosa

Biliana Lesic, Laurence G Rahme BMC Molecular Biology 2008, 9:20 (4 February 2008)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

14.

343
Accesses

Editorial   Open Access Highly Accessed

MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments

Stephen A Bustin, Jean-François Beaulieu, Jim Huggett, Rolf Jaggi, Frederick SB Kibenge, Pål A Olsvik, Louis C Penning, Stefan Toegel BMC Molecular Biology 2010, 11:74 (21 September 2010)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central | 1 comment |  Editor’s summary

The Editorial Board of BMC Molecular Biology endorse a new set of essential MIQE-light guidelines for the reporting of quantitative PCR data: "MIQE precis", and provide guidance for the suitability of pure reference gene papers to the journal.

15.

325
Accesses

Research article   Open Access Highly Accessed

Identification of differences in microRNA transcriptomes between porcine oxidative and glycolytic skeletal muscles

Yingkai Liu, Mingzhou Li, Jideng Ma, Jie Zhang, Chaowei Zhou, Tao Wang, Xiaolian Gao, Xuewei Li BMC Molecular Biology 2013, 14:7 (18 February 2013)

Abstract | Full text | PDF | PubMed |  Editor’s summary

Analysis of miRNA expression profiling between the porcine longissimus doris muscle  and psoas major muscle using a deep sequencing approach suggests that miRNAs play essential roles in the phenotypic variations observed in these muscle fiber types.

16.

314
Accesses

Methodology article   Open Access Highly Accessed

Selection of reference genes for gene expression studies in pig tissues using SYBR green qPCR

Ann-Britt Nygard, Claus B Jørgensen, Susanna Cirera, Merete Fredholm BMC Molecular Biology 2007, 8:67 (15 August 2007)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

17.

293
Accesses

Methodology article   Open Access Highly Accessed

RNA extraction from ten year old formalin-fixed paraffin-embedded breast cancer samples: a comparison of column purification and magnetic bead-based technologies

Alfredo Ribeiro-Silva, Haiyu Zhang, Stefanie S Jeffrey BMC Molecular Biology 2007, 8:118 (21 December 2007)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

18.

289
Accesses

Methodology article   Open Access Highly Accessed

Detection limits of several commercial reverse transcriptase enzymes: impact on the low- and high-abundance transcript levels assessed by quantitative RT-PCR

Jean-Philippe Levesque-Sergerie, Mathieu Duquette, Catherine Thibault, Louis Delbecchi, Nathalie Bissonnette BMC Molecular Biology 2007, 8:93 (22 October 2007)

Abstract | Full text | PDF | PubMed

19.

285
Accesses

Methodology article   Open Access Highly Accessed

Single cell transcriptomic analysis of prostate cancer cells

Christopher J Welty, Ilsa Coleman, Roger Coleman, Bryce Lakely, Jing Xia, Shu Chen, Roman Gulati, Sandy R Larson, Paul H Lange, Bruce Montgomery, Peter S Nelson, Robert L Vessella, Colm Morrissey BMC Molecular Biology 2013, 14:6 (16 February 2013)

Abstract | Full text | PDF | PubMed

20.

260
Accesses

Research article   Open Access Highly Accessed

Expression profiling and cross-species RNA interference (RNAi) of desiccation-induced transcripts in the anhydrobiotic nematode Aphelenchus avenae

Wesley Reardon, Sohini Chakrabortee, Tiago Pereira, Trevor Tyson, Matthew C Banton, Katharine M Dolan, Bridget A Culleton, Michael J Wise, Ann M Burnell, Alan Tunnacliffe BMC Molecular Biology 2010, 11:6 (19 January 2010)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central |  Editor’s summary

In response to falling humidity, the nematode Aphelenchus avenae upregulates a set of genes before entering a state of suspended animation, upregulated glutathione peroxidase making a demonstrable contribution to surviving extreme dessication.

21.

259
Accesses

Research article   Open Access Highly Accessed

Effect of experimental treatment on GAPDH mRNA expression as a housekeeping gene in human diploid fibroblasts

Azalina Zainuddin, Kien Chua, Norhazira Rahim, Suzana Makpol BMC Molecular Biology 2010, 11:59 (14 August 2010)

Abstract | Full text | PDF | PubMed

22.

258
Accesses

Research article   Open Access Highly Accessed

Selection of reliable reference genes for gene expression studies in peach using real-time PCR

Zhaoguo Tong, Zhihong Gao, Fei Wang, Jun Zhou, Zhen Zhang BMC Molecular Biology 2009, 10:71 (20 July 2009)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

23.

255
Accesses

Research article   Open Access Highly Accessed

Evaluation of putative reference genes for gene expression normalization in soybean by quantitative real-time RT-PCR

Ruibo Hu, Chengming Fan, Hongyu Li, Qingzhu Zhang, Yong-Fu Fu BMC Molecular Biology 2009, 10:93 (28 September 2009)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

24.

257
Accesses

Research article   Open Access

G-quadruplex recognition activities of E. Coli MutS

Edward A Ehrat, Bradley R Johnson, Jonathan D Williams, Glen M Borchert, Erik D Larson BMC Molecular Biology 2012, 13:23 (2 July 2012)

Abstract | Full text | PDF | PubMed

25.

250
Accesses

Methodology article   Open Access Highly Accessed

Identification of suitable endogenous control genes for microRNA gene expression analysis in human breast cancer

Pamela A Davoren, Roisin E McNeill, Aoife J Lowery, Michael J Kerin, Nicola Miller BMC Molecular Biology 2008, 9:76 (21 August 2008)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

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