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A single hydrophobic cleft in the Escherichia coli processivity clamp is sufficient to support cell viability and DNA damage-induced mutagenesis in vivo
Mark D Sutton, Jill M Duzen, Sarah K Scouten Ponticelli BMC Molecular Biology 2010, 11:102 (29 December 2010)
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The CTCF insulator protein forms an unusual DNA structure
Melissa J MacPherson, Paul D Sadowski BMC Molecular Biology 2010, 11:101 (21 December 2010)
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Editor’s summary
The zinc-finger repressor and insulator protein CTCF is able to form unusual DNA loop structures via its zinc finger domain in a directional manner and potentially function as a genome organizer.
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qPCR in gastrointestinal stromal tumors: Evaluation of reference genes and expression analysis of KIT and the alternative receptor tyrosine kinases FLT3, CSF1-R, PDGFRB, MET and AXL
Jana Fassunke, Marie-Christine Blum, Hans-Ulrich Schildhaus, Marc Zapatka, Benedikt Brors, Helen Künstlinger, Reinhard Büttner, Eva Wardelmann, Sabine Merkelbach-Bruse BMC Molecular Biology 2010, 11:100 (20 December 2010)
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A thymus-specific noncoding RNA, Thy-ncR1, is a cytoplasmic riboregulator of MFAP4 mRNA in immature T-cell lines
Kazuma Aoki, Akira Harashima, Miho Sano, Takahide Yokoi, Shuji Nakamura, Masayoshi Kibata, Tetsuro Hirose BMC Molecular Biology 2010, 11:99 (16 December 2010)
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Editor’s summary
The novel thymus-specific noncoding RNA Thy-ncR1, whose precursor exhibits complex alternative splicing patterns, has a cytoplasmic localization and functions as a riboregulator of mRNA stability during a specific stage of T-cells maturation.
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Characterization, phylogeny, alternative splicing and expression of Sox30 gene
Fei Han, Zhijian Wang, Fengrui Wu, Zhihao Liu, Baofeng Huang, Deshou Wang BMC Molecular Biology 2010, 11:98 (11 December 2010)
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Structure and expression of two nuclear receptor genes in marsupials: insights into the evolution of the antisense overlap between the α-thyroid hormone receptor and Rev-erbα
Brandon C Rindfleisch, M Scott Brown, John L VandeBerg, Stephen H Munroe BMC Molecular Biology 2010, 11:97 (10 December 2010)
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Identifying novel genes in C. elegans using SAGE tags
Matthew J Nesbitt, Donald G Moerman, Nansheng Chen BMC Molecular Biology 2010, 11:96 (10 December 2010)
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Overexpression of miR-128 specifically inhibits the truncated isoform of NTRK3 and upregulates BCL2 in SH-SY5Y neuroblastoma cells
Monica Guidi, Margarita Muiños-Gimeno, Birgit Kagerbauer, Eulàlia Martí, Xavier Estivill, Yolanda Espinosa-Parrilla BMC Molecular Biology 2010, 11:95 (10 December 2010)
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A mechanism for ramified rolling circle amplification
Thomas P Beals, James H Smith, Raymond M Nietupski, David J Lane BMC Molecular Biology 2010, 11:94 (7 December 2010)
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Cell- and region-specific miR30-based gene knock-down with temporal control in the rat brain
Beihui Liu, Haibo Xu, Julian FR Paton, Sergey Kasparov BMC Molecular Biology 2010, 11:93 (6 December 2010)
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Rrd1 isomerizes RNA polymerase II in response to rapamycin
Nathalie Jouvet, Jeremie Poschmann, Julie Douville, Lisa Bulet, Dindial Ramotar BMC Molecular Biology 2010, 11:92 (3 December 2010)
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Essential role for ALCAM gene silencing in megakaryocytic differentiation of K562 cells
Fang Tan, Samit Ghosh, Flaubert Mbeunkui, Robert Thomas, Joshua A Weiner, Solomon F Ofori-Acquah BMC Molecular Biology 2010, 11:91 (2 December 2010)
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Selection of reliable reference genes during THP-1 monocyte differentiation into macrophages
Marten B Maeß, Stefanie Sendelbach, Stefan Lorkowski BMC Molecular Biology 2010, 11:90 (1 December 2010)
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Subcellular distribution of nuclear import-defective isoforms of the promyelocytic leukemia protein
Åsne Jul-Larsen, Amra Grudic, Rolf Bjerkvig, Stig O Bøe BMC Molecular Biology 2010, 11:89 (21 November 2010)
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Expression of 3-hydroxy-3-methylglutaryl-CoA reductase, p-hydroxybenzoate-m-geranyltransferase and genes of phenylpropanoid pathway exhibits positive correlation with shikonins content in arnebia [Arnebia euchroma (Royle) Johnston]
Ravi S Singh, Rishi K Gara, Pardeep K Bhardwaj, Anish Kaachra, Sonia Malik, Ravi Kumar, Madhu Sharma, Paramvir S Ahuja, Sanjay Kumar BMC Molecular Biology 2010, 11:88 (21 November 2010)
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Translational independence between overlapping genes for a restriction endonuclease and its transcriptional regulator
Meenakshi K Kaw, Robert M Blumenthal BMC Molecular Biology 2010, 11:87 (19 November 2010)
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Germline transformation of the stalk-eyed fly, Teleopsis dalmanni
Ian A Warren, Kevin Fowler, Hazel Smith BMC Molecular Biology 2010, 11:86 (16 November 2010)
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A tryptophan-rich peptide acts as a transcription activation domain
Chen-Huan Lin, Grace Lin, Chia-Pei Chang, Chien-Chia Wang BMC Molecular Biology 2010, 11:85 (16 November 2010)
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Targeting of highly conserved Dengue virus sequences with anti-Dengue virus trans-splicing group I introns
James R Carter, James H Keith, Pradip V Barde, Tresa S Fraser, Malcolm J Fraser BMC Molecular Biology 2010, 11:84 (15 November 2010)
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Identification of amino acid residues in protein SRP72 required for binding to a kinked 5e motif of the human signal recognition particle RNA
Elena Iakhiaeva, Alexei Iakhiaev, Christian Zwieb BMC Molecular Biology 2010, 11:83 (13 November 2010)
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Editor’s summary
The binding requirements of the protein and RNA components of the human signal recognition particle (SRP), the ribonucleoprotein complex that binds to the signal sequences of secretory polypeptides, have been elucidated and a model for ligand binding proposed.
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Single-cell qPCR on dispersed primary pituitary cells -an optimized protocol
Kjetil Hodne, Trude M Haug, Finn-Arne Weltzien BMC Molecular Biology 2010, 11:82 (12 November 2010)
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Regulation of PURA gene transcription by three promoters generating distinctly spliced 5-prime leaders: a novel means of fine control over tissue specificity and viral signals
Margaret J Wortman, Laura K Hanson, Luis Martínez-Sobrido, Ann E Campbell, Jonas A Nance, Adolfo García-Sastre, Edward M Johnson BMC Molecular Biology 2010, 11:81 (9 November 2010)
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Sex-biased transcription enhancement by a 5' tethered Gal4-MOF histone acetyltransferase fusion protein in Drosophila
Anja H Schiemann, Fang Li, Vikki M Weake, Esther J Belikoff, Kent C Klemmer, Stanley A Moore, Maxwell J Scott BMC Molecular Biology 2010, 11:80 (9 November 2010)
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Regulation of CEACAM1 transcription in human breast epithelial cells
Marieta Gencheva, Charng-Jui Chen, Tung Nguyen, John E Shively BMC Molecular Biology 2010, 11:79 (4 November 2010)
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Identification of a variant form of tyrosine phosphatase LYP
Shaofeng Wang, Hongbo Dong, Jiayu Han, Wanting T Ho, Xueqi Fu, Zhizhuang J Zhao BMC Molecular Biology 2010, 11:78 (2 November 2010)
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