Human intronic enhancer elements control Gli3 expression in the mouse central nervous system
GLI3, a zinc finger transcription factor, mediates Sonic hedgehog signaling and patterning of the vertebrate body plan. To examine how genetic elements affect the spatio-temporal regulation of Gli3 expression, mouse transgenic assays were performed to examine how human conserved non-coding sequence elements (CNE) activated reporter gene expression. At embryonic day E11.5, CNE1-controlled lacZ was strongly expressed in the dorsal brain and spinal cord (blue), and less prominently in hypaxial buds of the thoracic somites, proximal muscle masses in the forelimb bud, dorsal root ganglion, and in the facial mesenchyme. Whole mount X-gal staining of histological sections of transgenic mouse embryos show reporter activation (blue) under the control of CNE1.
Taken from: Amir Abbasi et al., 2010, BMC Developmental Biology [View article]
To view related images from across BioMed Central, visit the Biology Image Library.