Remodeling of the pancreatic epithelial cell mass into polarized monolayers
To obtain a better insight into the regulation of branching morphogenesis in the developing pancreas in mice, primitive duct morphogenesis and epithelial cell polarization were analyzed. Pancreatic development starts with a mass of non-polarized epithelial cells and by e14.5, epithelial cells (red) become polarized, with their basal pole contacting laminin (blue) and apical pole facing a lumen (green). Pancreatic sections from e14.5 embryos were examined by immunofluorescence using antibodies directed against E-cadherin (red), mucin-1 (green) and laminin (blue), with overlap of signal in yellow.
Taken from: Christophe Pierreux et al., 2009, BMC Developmental Biology [View article]