Undersized eye of zebrafish embryo with a mutated condensin gene
. The condensin I protein CAP-G, which functions in chromosome condensation and segregation, is encoded by the creature from the black lagoon (cbl) locus in Zebrafish, and embryos bearing a mutant allele of this locus (cbls105) show mitotic defects and a severe size reduction of the retina and head compared with wild-type embryos. Mutant embryos at 3 days post fertilization still have the three layers of the eye containing differentiated cells (ganglion; amacrine, bipolar and horizontal cells, and photoreceptor cells in the outermost layer) but the number of retinal cells are reduced by half compared with wild-type eyes. Transverse sections through the retinae were stained with phalloidin (to visualise the plexiform layers of differentiating cells: red) and propidium iodide to mark nuclei (green) with a little overlap of signal in yellow.
Taken from: Salim Abdelilah-Seyfried et al., 2009, BMC Developmental Biology [View article]