sds22 role in Drosophila epithelial morphology
A mutant clone from a mutagenesis screen in Drosophila disrupts epithelial morphology, due to mutation of the sds22 gene, which encodes a PP1 phosphatase regulatory subunit. In the wing imaginal disc, sds22 mutant clones were induced at 84 hrs after egg laying and mutant clone growth was marked by lack of GFP expression (green) 2 days later. Extrusion of the sds22 mutant cells left behind dramatic folds in the epithelium, more easily identified when the filamentous actin cytoskeleton (red) was visualized after staining with phalloidin, and nuclei (blue) following staining with DAPI.
Taken from: Barry Thompson et al., 2009, BMC Developmental Biology [View article]
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