Mek 1/2-related defects in zebrafish notochord development
Treatment of zebrafish embryos with the Mek 1/2 inhibitor (U0126) disrupts notochord development, resulting in a short tail and bent notochord. This is due to changes in actin-based cytoskeleton and structures of the perinotochordal basement membrane, and not due to a lack of laminin protein or its mislocalization. Laminin expression was measured by whole-mount immunohistochemistry (green fluorescence) in treated embryos at 48 h post-fertilization.
Taken from: Zsolt Lele et al., 2008, BMC Developmental Biology [View article]