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Risk factors for hemorrhagic cystitis in children undergoing hematopoietic stem cell transplantation: a systematic review and meta-analysis
BMC Pediatrics volume 24, Article number: 333 (2024)
Abstract
Background
The risk factors for hemorrhagic cystitis (HC) in children undergoing hematopoietic stem cell transplantation (HSCT) are unclear. Therefore, we conducted this systematic review and meta-analysis to investigate the risk factors for HC in children undergoing HSCT.
Methods
We performed this meta-analysis by retrieving studies from PubMed, EMBASE, and the Cochrane Library up to October 10, 2023, and analyzing those that met the inclusion criteria. I2 statistics were used to evaluate heterogeneity.
Results
Twelve studies, including 2,764 patients, were analyzed. Male sex (odds ratio [OR] = 1.52; 95% confidence interval [CI], 1.16–2.00; p = 0.003, I2 = 0%), allogeneic donor (OR = 5.28; 95% CI, 2.60–10.74; p < 0.00001, I2 = 0%), human leukocyte antigen (HLA) mismatched donor (OR = 1.86; 95% CI, 1.00–3.44; p = 0.05, I2 = 31%), unrelated donor (OR = 1.58; 95% CI, 1.10–2.28; p = 0.01, I2 = 1%), myeloablative conditioning (MAC) (OR = 3.17; 95% CI, 1.26–7.97; p = 0.01, I2 = 0%), busulfan (OR = 2.18; 95% CI, 1.33–3.58; p = 0.002, I2 = 0%) or anti-thymoglobulin (OR = 1.65; 95% CI, 1.07–2.54; p = 0.02, I2 = 16%) use, and cytomegalovirus (CMV) reactivation (OR = 2.64; 95% CI, 1.44–4.82; p = 0.002, I2 = 0%) were risk factors for HC in children undergoing HSCT.
Conclusions
Male sex, allogeneic donor, HLA-mismatched, unrelated donor, MAC, use of busulfan or anti-thymoglobulin, and CMV reactivation are risk factors for HC in children undergoing HSCT.
Background
Hematopoietic stem cell transplantation (HSCT) is an effective therapeutic approach for various hematologic malignant and nonmalignant diseases [1]. However, the range of complications that arise following HSCT remains a challenge.
Hemorrhagic cystitis (HC) is a frequent complication in patients undergoing HSCT, significantly diminishing their quality of life and extending the length of their hospital stay [2]. Moreover, severe HC is associated with higher mortality [3]. HC is categorized into two groups based on its onset time: early-onset (within 72Â h) and late-onset (more than 72Â h after conditioning) [4]. The reported incidence rates of HC in pediatric HSCT recipients vary between 3% and 27% [5, 6]. The clinical presentation of HC varies. Mild cases may manifest only as microscopic hematuria, whereas severe cases can manifest as life-threatening complications characterized by continuous bleeding and urinary tract obstruction [7]. The risk factors for HC include age, receipt of cells from an unrelated donor, and the occurrence of acute graft-versus-host disease (GVHD) [8, 9]. However, the conclusions have been inconsistent [10,11,12], and the risk factors for HC in children undergoing HSCT remain elusive. Therefore, we conducted this systematic review and meta-analysis to further investigate the risk factors for HC, with the aim of providing clinical evidence for early identification and prevention and improving the quality of life of pediatric patients with HC.
Methods
This study was conducted in accordance with the recommendations of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines [13].
Literature search strategy
The PubMed, EMBASE, and the Cochrane Library databases were searched up to October 10, 2023 by two independent reviewers (Zhang and Wang) for relevant references. Relevant studies were identified using MeSH terms and keywords for cystitis, HSCT, child, pediatric, and adolescent. A detailed literature search process in PubMed is shown in the Supplemental File. The references cited by the included articles were also retrieved to identify other potentially eligible studies. A third reviewer (Fan) was consulted to resolve any disagreements in this process.
Inclusion and exclusion criteria
The studies were included if they met all the following inclusion criteria: (1) retrospective or prospective original studies published in English; (2) performed among pediatric and young patients aged 21 years or younger; (3) clearly included an HC group and a no HC group; (4) provided sufficient information on the risk factors for HC. Studies were excluded if they met any of the following exclusion criteria: (1) included fewer than five patients in any group; (2) investigated only one category of HC; (3) did not report at least one risk factor for HC; or (4) were reviews, letters, conferences, comments, or case reports.
Data extraction and quality assessment
A standardized Excel table was used by two investigators to collect the baseline data of the included studies. The data included the first author, publication year, geographic region, study accrual period, study design, sample size of each group, definition of HC, and risk factors reported in the included studies. Two reviewers independently applied the Newcastle–Ottawa scale (NOS) to assess the quality of the included studies. The quality of the study was rated high if the final NOS score was > 7. Any discrepancies in these two processes were resolved through discussion with a third reviewer.
Statistical analysis
All statistical analyses included in this meta-analysis were performed using Cochrane Review Manager software (RevMan version 5.4). The odds ratios (ORs) and 95% confidence intervals (CIs) were calculated to estimate the roles of all potential risk factors in the development of HC. The heterogeneity of each result was tested using the Cochrane Q test and I2 statistics. An I2 value < 50% and a P value > 0.1 indicated non-significant heterogeneity, and a fixed-effects model was used. In contrast, an I2 value > 50% or a P value < 0.1 indicated significant heterogeneity, and a random-effects model was used. In addition, the publication bias of the included studies was evaluated using funnel plots of each risk factor. An asymmetrical shape of the funnel plot indicates publication bias.
Results
Study selection and characteristics
A total of 515 references were included after the initial search using our search terms (77 in PubMed, 419 in EMBASE, and 19 in the Cochrane Library). After removing 69 duplicate references, the remaining 446 references were further screened by reading the title and abstract. A total of 39 studies were eligible for full-text review, and 12 were included in this meta-analysis [5, 6, 9,10,11, 14,15,16,17,18,19,20]. One study [21] was excluded because it contained data duplicated from another study included in our meta-analysis [15]; therefore, we included the newest data in the analysis. The selection flowchart of the included studies is shown in Fig. 1. Of the 12 included studies, nine were retrospective, and three were prospective, with the publication year ranging from 1998 to 2023. A total of 2,760 pediatric HSCT recipients were included in this meta-analysis, with 290 patients in the HC group and 2,470 patients in the no HC group. Table 1 presents the baseline characteristics and NOS scores for each study. The NOS scores ranged from 6 to 9. Therefore, the quality of the included studies was rated as moderate to high (Table S1). The funnel plots of all risk factors were nearly symmetrical, indicating no publication bias (Figure S1). Seventeen potential risk factors were evaluated in the meta-analysis.
Incidence and risk factors for HC in this meta-analysis
Among the included studies, the overall incidence rate of HC was 9.9% (276 of 2,764). Male sex, allogeneic donor, human leukocyte antigen (HLA) mismatched donor, unrelated donor, myeloablative conditioning (MAC), busulfan and anti-thymocyte globulin (ATG) use, and cytomegalovirus (CMV) reactivation were risk factors for HC in children undergoing HSCT. Although receiving reduced-intensity conditioning (RIC) was associated with a decreased risk of HC, malignant disease, source of stem cells, use of pre-transplantation cyclophosphamide or total body irradiation (TBI), acute GVHD, and chronic GVHD were not significant risk factors for HC. The pooled results of all potential risk factors are presented in Table 2, and the forest plots of non-significant risk factors are presented in the Supplemental File.
Male sex
Eleven studies, including 2,575 patients, assessed the association between male sex and the occurrence of HC. The analysis revealed that male patients were at a higher risk of developing HC than female patients (OR = 1.52; 95% CI, 1.16–2.00; p = 0.003) (Fig. 2). There was low heterogeneity among the 11 studies (I2 = 0, p = 0.75), so a fixed-effects model was used to pool the results.
Malignant disease
Eight studies, including 2,088 patients, were analyzed. The results showed no association between malignant disease and the risk of developing HC in children undergoing HSCT (OR = 1.26; 95% CI, 0.83–1.90; p = 0.28) (Figure S2). There was no significant heterogeneity (I2 = 33, p = 0.16); therefore, a fixed-effects model was used to pool the results.
Allogenic donor
Two studies, including 807 patients, assessed the association between having an allogenic donor and the risk of developing HC in children undergoing HSCT. Patients with an allogenic donor had a higher risk of developing HC (OR = 5.28; 95% CI, 2.60–10.4; p < 0.00001) (Fig. 2). A fixed effects model was used because there was no significant heterogeneity (I2 = 0, p = 0.38).
Sources of stem cell
The pooled results indicated that there was no association between the source of bone marrow transplantation and HC in children undergoing HSCT, with no heterogeneity (OR = 1.10; 95% CI, 0.69–1.76; p = 0.69; I2 = 0, n = 1263) (Figure S3). Similarly, there were no statistically significant differences between peripheral blood transplantation (OR = 0.56; 95% CI, 0.29–1.11; p = 0.10; I2 = 0, n = 1584) (Figure S4) or cord blood transplantation (OR = 1.72; 95% CI, 0.87–3.40; p = 0.12; I2 = 16, n = 1159) (Figure S5) and the risk of developing HC in children undergoing HSCT, with no heterogeneity.
HLA-mismatched donor
Analysis of three studies including 276 patients showed that in children undergoing HSCT, patients with an HLA-mismatched donor had a higher risk of developing HC than those with matched donors (OR = 1.86; 95% CI, 1.00–3.44; p = 0.05) (Fig. 2). A fixed-effects model was used, as there was no significant heterogeneity (I2 = 31, p = 0.23) among the studies.
Unrelated donor
Nine studies, including 1,159 patients, were analyzed. The analysis revealed that in children undergoing HSCT, patients who received cells from an unrelated donor had a higher risk of developing HC (OR = 1.58; 95% CI, 1.10–2.28; p = 0.01) (Fig. 2). A fixed effects model was used because there was no significant heterogeneity (I2 = 1, p = 0.43).
Conditioning regimen
Regarding the conditioning regimen, MAC was associated with an increased risk of developing HC in children undergoing HSCT (OR = 3.19; 95% CI, 1.28–7.96; p = 0.01) (Fig. 3), whereas receiving RIC was associated with a reduced risk of developing HC (OR = 0.31; 95% CI, 0.13–0.78; p = 0.01) (Figure S6), with no heterogeneity (I2 = 0, n = 259).
Conditioning agents in HSCT
Regarding the agents used in the conditioning regimen, the use of busulfan (OR = 2.18; 95% CI, 1.33–3.58; p = 0.002) (Fig. 3) or ATG (OR = 1.65; 95% CI, 1.07–2.54; p = 0.02) (Fig. 3) was associated with an increased risk of developing HC in children undergoing HSCT, with no heterogeneity (busulfan: I2 = 0, n = 770; and ATG: I2 = 16, n = 858). In contrast, the use of pre-transplantation cyclophosphamide (OR = 1.02; 95% CI, 0.69–1.51; p = 0.92) (Figure S7) or TBI (OR = 0.94; 95% CI, 0.65–1.36; p = 0.76) (Figure S8) was not associated with the risk of developing HC in children undergoing HSCT, with no heterogeneity (cyclophosphamide: I2 = 45, n = 1843; and TBI: I2 = 0, n = 2180).
Acute and chronic GVHD
Neither acute GVHD (OR = 1.45; 95% CI, 0.64–3.26; p = 0.37) (Figure S9) nor chronic GVHD (OR = 0.67; 95% CI, 0.27–1.68; p = 0.39) (Figure S10) was associated with an increased risk of developing HC in children undergoing HSCT. However, there was significant heterogeneity among the included studies (acute GVHD: I2 = 61, n = 809; and chronic GVHD: I2 = 61, n = 725); therefore, random effects models were used to pool the results.
CMV reactivation
Only two studies, including 320 patients, investigated the relationship between CMV reactivation and HC development. Patients with CMV reactivation had an increased risk of HC after undergoing HSCT (OR = 2.65; 95% CI, 1.45–4.84; p = 0.001) (Fig. 3). A fixed-effects model was used, as there was no significant heterogeneity (I2 = 0, p = 0.73).
Discussion
This is the first meta-analysis to explore the risk factors for HC in children undergoing HSCT. Zhou et al. [22] reported the risk factors related to BK virus-associated HC (BKV-HC). They found that male sex, haploidentical donor, MAC, acute GVHD, chronic GVHD, and CMV reactivation were risk factors for BKV-HC in patients who underwent allogeneic HSCT. Owing to the limited number of included studies, they did not perform a subgroup analysis of pediatric patients and only investigated virus-induced HC. Therefore, the risk factors for pediatric patients undergoing HSCT remain unclear. This meta-analysis included 12 studies involving 2,764 patients that explored the risk factors for HC in children undergoing HSCT, and 17 potential risk factors were evaluated. The results of the pooled analysis indicated that male sex, allogeneic donor, HLA-mismatched donor, unrelated donor, MAC, use of busulfan or ATG, and CMV reactivation were risk factors for HC in children undergoing HSCT.
Our study revealed that boys were more likely to develop HC after HSCT than girls, which is consistent with the results of previous studies [9, 23, 24]. A possible reason for this finding may be that estrogen exerts a protective effect in the bladder mucosa, which contributes to a lower incidence of HC in female HSCT recipients than in male recipients [25].
In our analysis, no significant differences were observed in the risk of HC between patients with malignant and nonmalignant diseases. All studies included in this meta-analysis consistently indicated that patients with malignant disease did not exhibit a higher risk of HC than those with nonmalignant disease. However, in patients with malignant disease, intensive chemotherapy may cause injury to the bladder mucosa and contribute to the occurrence of HC after HSCT. Although our analysis did not suggest that malignant disease is a risk factor for HC in children undergoing HSCT, further studies are warranted to explore this relationship.
The types of HSCT include autologous and allogeneic, owing to the different sources of stem cells (the patients or others). Recipients of allogeneic transplantation may have a higher incidence of HC [14, 26], which is consistent with our results. We speculate that this association may be attributable to the higher dose of cyclophosphamide (which can injure the bladder mucosa) employed in the conditioning regimen of allogeneic transplant recipients. However, the reliability of this finding is constrained due to the inclusion of only two studies. Further studies with large sample sizes are warranted to elucidate the relationship between HC and the type of HSCT.
The stem cell sources generally included bone marrow, peripheral blood, and cord blood. The results revealed that the risk of HC did not differ significantly according to the source of stem cells, which is consistent with the findings of previous studies [10, 18, 27]. Although our analysis did not find an association between cord blood transplantation and the risk of HC in pediatric HSCT recipients, Jiang et al. [28] reported that HLA matching ≤ 6/8 was an independent risk factor for the occurrence of late-onset HC. HLA compatibility of cord blood units may play an essential role in the relationship between cord blood transplantation and the occurrence of HC. However, we could not assess this relationship owing to the limited data available. Further studies are required to assess the relationship between HLA mismatching and the risk of HC.
Previous studies have reported that the risk of HC is higher in HSCT recipients who receive cells from an unrelated donor [19, 29], which is consistent with the findings of our study. However, other studies have found that an HLA-mismatched donor is not a risk factor for HC [5, 15]. Our analysis results indicated that receiving cells from both HLA-mismatched and unrelated donors was associated with an increased risk of HC. This may be because recipients of cells from an unrelated donor require more intensive immunosuppression therapy, which may contribute to the development of HC [2]. Therefore, a matched-related donor is preferred to prevent the occurrence of HC in children undergoing HSCT. However, this conclusion may be unreliable owing to the limited number of studies included, and further high-quality studies are warranted to further explore the relationship between HLA-mismatched donors and HCs.
According to the different intensities of immunosuppression, the conditioning regimen was divided into two general categories: MAC and RIC. The HC risk was higher in patients who received MAC than in those who received RIC, which is consistent with the findings of several previous studies [5, 30]. This finding can be attributed to the lower toxicity of RIC, which causes milder damage to the mucosa of the bladder and, subsequently, a lower incidence of HC. Cyclophosphamide administration has previously been reported to be associated with an increased risk of HC [31]; however, our results indicate that it does not increase the risk of HC [31]. This may be because most of the studies included in this analysis investigated late-onset HC, whereas cyclophosphamide is more often associated with early-onset HC [32]. Busulfan, an alkylating agent commonly used in conditioning regimens, has previously been reported to be associated with an increased risk of HC [6], which is consistent with the results of this meta-analysis. The increased risk of HC associated with busulfan use may be attributable to the urothelial changes caused by busulfan [33]. The use of ATG was also associated with an increased risk of HC in this meta-analysis. This may be attributable to the higher risk of viral infections in patients who receive this drug [34]. Similarly, Fu et al. [35] suggested that a higher dose of ATG may be associated with an increased risk of HC in haploidentical HSCT recipients. TBI is another key component of conditioning for HSCT. Several previous studies have not found an association between TBI and the risk of HC [12, 18, 27], which is consistent with the results of our analysis.
GVHD is another common complication in patients undergoing HSCT, which limits the use of HSCT and is a leading cause of morbidity and mortality [36]. Acute GVHD has been reported to be associated with an increased risk of HC [5]. This may be attributable to the immunosuppressive therapy used for treating acute GVHD, which increases the risk of viral infection and subsequently causes the occurrence of HC. Alternatively, HC may be a manifestation of GVHD, which generally involves multiple systems [37]. However, the pooled results of our meta-analysis indicate that acute GVHD does not increase the risk of HC. However, this finding may be unreliable owing to the limited number of included studies and the significant heterogeneity between studies. Several previous studies have explored the relationship between chronic GVHD and HC but have found no association [9, 19], which is consistent with our results. However, this finding may be unreliable owing to the limited data available in the included studies and the relatively high heterogeneity between studies.
Our analysis indicated that CMV reactivation was a risk factor for HC in HSCT recipients. Zhang et al. [38] reported that the incidence rate of HC was higher in patients with CMV infection than in those without CMV infection. This may be because CMV infection causes impaired T-cell immunity and weak T-cell responses, which contribute to an increased risk of BKV infection and, consequently, the occurrence of HC [39]. However, this finding may be unreliable owing to the small number of studies included in the analysis. Further studies with large sample sizes are warranted to further explore the relationship between CMV reactivation and HC.
Owing to the limited data available in the included studies, we could not analyze the risk factors for the severity of HC. However, Leung et al. [40] reported that a 3-log increase in the BKV load in adult patients undergoing HSCT was an independent risk factor for severe HC. Another recently published study showed that BKV infection and multiple infections were predictors of HC severity in children undergoing HSCT [41]. Ost et al. [37] reported that the severity of acute GVHD was related to the severity of HC. BKV infection and GVHD may play key roles in the development of HC and cause more severe disease. Therefore, managing BKV infection and GVHD is important for preventing HC in children undergoing HSCT.
Other potential risk factors reported in previous studies include age and T-cell depletion. However, owing to the limited data available and the lack of standardization of reporting, these factors were not investigated in this meta-analysis. Previous studies have reported that in pediatric HSCT recipients, the incidence of HC is lower in younger children [6, 11, 42]. This may be attributable to the lower incidence of viral infection in younger children. Regarding T-cell depletion, a previous study found that patients with T-cell depletion had a higher risk of developing HC [9]. This may be because T-cell depletion results in substantial immunosuppression, which increases the risk of viral infection and HC. Further high-quality prospective studies are warranted to further explore the relationship between these factors and the development of HC.
Therapeutic options for HC are diverse and differ according to the disease severity and the presence of viral infection. Preventive therapy and conservative and supportive therapy should be used as the first steps in non-severe cases. In patients with more severe HC, continuous bladder irrigation and intravesical therapy may be considered. Moreover, in very severe cases, surgical procedures may be required to remove clots and stop bleeding [8]. Cidofovir, an antiviral drug, has high specificity against the BKV and has been shown to be effective in preventing and treating BKV-HC [43, 44]. Other antiviral drugs, such as leflunomide, have also been reported to be effective against BKV-HC [45, 46]. Virus-specific T cells (VSTs), which are derived from a patient’s stem cell donor (donor-derived) or a third-party donor, are a safe and effective therapy for viral infections in children undergoing HSCT [47, 48]. In a cohort of 56 patients undergoing HSCT, gross hematuria was completely resolved in 13 of 14 patients with BKV-HC who received VST infusion for six weeks, whereas four of the five patients who did not receive VST infusion developed disease progression [49]. Another clinical trial conducted by Olson et al. [50] reported that the clinical response of patients with HC who received VST was better than that of historical controls. These findings indicate that VSTs may be a feasible option for treating HC, especially BKV-HC, in patients undergoing HSCT. Other treatment strategies, such as estrogen, hyperbaric oxygen therapy, and mesenchymal stromal cells, have also been reported to be effective and can be used as alternative strategies.
This meta-analysis has several limitations. First, combining prospective and retrospective studies may result in potential bias. Second, owing to the limited number of included studies, it was not possible to perform multivariate or subgroup analyses. Third, the large time span of included studies and different transplantation protocols may limit the generalizability of our findings. Fourth, the criteria for the diagnosis of HC differed in different studies, which may contribute to bias.
Conclusions
Our meta-analysis suggested that male sex, allogeneic donor, HLA-mismatched donor, unrelated donor, MAC, use of busulfan or ATG, and CMV reactivation were potential risk factors for HC in pediatric HSCT recipients. However, additional prospective studies with large sample sizes are required to further explore the risk factors for HC in children undergoing HSCT.
Data availability
All data generated or analyzed during this study are included in this published article [and its supplementary information files].
Abbreviations
- ATG:
-
Anti-thymocyte globulin
- BKV:
-
BK virus
- CI:
-
Confidence interval
- CMV:
-
Cytomegalovirus
- GVHD:
-
Graft versus host disease
- HC:
-
Hemorrhagic cystitis
- HLA:
-
Human leucocyte antigen
- HSCT:
-
Hematopoietic stem cell transplantation
- MAC:
-
Myeloablative conditioning
- NOS:
-
Newcastle Ottawa Scale
- OR:
-
Odds ratio
- RIC:
-
Reduced-intensity conditioning
- VST:
-
Virus-specific T-cells
References
Zhang XH, Chen J, Han MZ, Huang H, Jiang EL, Jiang M, et al. The consensus from the Chinese Society of Hematology on indications, conditioning regimens and donor selection for allogeneic hematopoietic stem cell transplantation: 2021 update. J Hematol Oncol. 2021;14:145.
El-Zimaity M, Saliba R, Chan K, Shahjahan M, Carrasco A, Khorshid O, et al. Hemorrhagic cystitis after allogeneic hematopoietic stem cell transplantation: donor type matters. Blood. 2004;103:4674–80.
Ruggeri A, Roth-Guepin G, Battipaglia G, Mamez AC, Malard F, Gomez A, et al. Incidence and risk factors for hemorrhagic cystitis in unmanipulated haploidentical transplant recipients. Transpl Infect Dis. 2015;17:822–30.
Miano M, Faraci M, Dini G, Bordigoni P. Early complications following haematopoietic SCT in children. Bone Marrow Transpl. 2008;41(Suppl 2):S39–42.
Li B, Meng L, Tian Y, Lu Q, Gao L, Xiao P, et al. Outcomes and risk factors of hemorrhagic cystitis in pediatric allogeneic hematopoietic stem cell transplantation recipients using different graft source and condition with severe aplastic anemia. Hematology. 2022;27:714–22.
Kondo M, Kojima S, Kato K, Matsuyama T. Late-onset hemorrhagic cystitis after hematopoietic stem cell transplantation in children. Bone Marrow Transpl. 1998;22:995–8.
Leung AY, Suen CK, Lie AK, Liang RH, Yuen KY, Kwong YL. Quantification of polyoma BK Viruria in hemorrhagic cystitis complicating bone marrow transplantation. Blood. 2001;98:1971–8.
Harkensee C, Vasdev N, Gennery AR, Willetts IE, Taylor C. Prevention and management of BK-virus associated haemorrhagic cystitis in children following haematopoietic stem cell transplantation–a systematic review and evidence-based guidance for clinical management. Br J Haematol. 2008;142:717–31.
Hale GA, Rochester RJ, Heslop HE, Krance RA, Gingrich JR, Benaim E, et al. Hemorrhagic cystitis after allogeneic bone marrow transplantation in children: clinical characteristics and outcome. Biol Blood Marrow Transpl. 2003;9:698–705.
Cheuk DK, Lee TL, Chiang AK, Ha SY, Lau YL, Chan GC. Risk factors and treatment of hemorrhagic cystitis in children who underwent hematopoietic stem cell transplantation. Transpl Int. 2007;20:73–81.
Kloos RQ, Boelens JJ, de Jong TP, Versluys B, Bierings M. Hemorrhagic cystitis in a cohort of pediatric transplantations: incidence, treatment, outcome, and risk factors. Biol Blood Marrow Transpl. 2013;19:1263–6.
Hayden RT, Gu Z, Liu W, Lovins R, Kasow K, Woodard P, et al. Risk factors for hemorrhagic cystitis in pediatric allogeneic hematopoietic stem cell transplant recipients. Transpl Infect Dis. 2015;17:234–41.
Page MJ, McKenzie JE, Bossuyt PM, Boutron I, Hoffmann TC, Mulrow CD, et al. The PRISMA 2020 statement: an updated guideline for reporting systematic reviews. BMJ. 2021;372:n71.
Cesaro S, Brugiolo A, Faraci M, Uderzo C, Rondelli R, Favre C, et al. Incidence and treatment of hemorrhagic cystitis in children given hematopoietic stem cell transplantation: a survey from the Italian association of pediatric hematology oncology-bone marrow transplantation group. Bone Marrow Transpl. 2003;32:925–31.
Cesaro S, Tridello G, Pillon M, Calore E, Abate D, Tumino M, et al. A prospective study on the predictive value of plasma BK Virus-DNA load for Hemorrhagic Cystitis in Pediatric patients after Stem Cell transplantation. J Pediatr Infect Dis Soc. 2015;4:134–42.
Gorczynska E, Turkiewicz D, Rybka K, Toporski J, Kalwak K, Dyla A, et al. Incidence, clinical outcome, and management of virus-induced hemorrhagic cystitis in children and adolescents after allogeneic hematopoietic cell transplantation. Biol Blood Marrow Transplant. 2005;11:797–804.
Hayden RT, Gu Z, Liu W, Lovins R, Kasow K, Woodard P, et al. Risk factors for hemorrhagic cystitis in pediatric allogeneic hematopoietic stem cell transplant recipients. Transpl Infect Disease. 2015;17:234–41.
Laskin BL, Denburg M, Furth S, Diorio D, Goebel J, Davies SM, et al. BK Viremia precedes hemorrhagic cystitis in children undergoing allogeneic hematopoietic stem cell transplantation. Biol Blood Marrow Transpl. 2013;19:1175–82.
Rostami T, Aghabeigi S, Kiumarsi A, Kasaeian A, Parizi MK, Mirhosseini A et al. Incidence of hemorrhagic cystitis following unmanipulated peripheral blood stem cell transplantation in acute Leukemia: A retrospective single-center risk factor analysis. J Pediatr Urol. 2023;19:54.e1-54.e8.
Uppugunduri CRS, Storelli F, Mlakar V, Huezo-Diaz Curtis P, Rezgui A, Théorêt Y, et al. The Association of Combined GSTM1 and CYP2C9 genotype status with the occurrence of Hemorrhagic Cystitis in Pediatric patients receiving myeloablative conditioning Regimen Prior to Allogeneic hematopoietic stem cell transplantation. Front Pharmacol. 2017;8:451.
Cesaro S, Pillon M, Tridello G, Aljurf M, Martino R, Schroyens W, et al. Relationship between clinical and BK virological response in patients with late hemorrhagic cystitis treated with cidofovir: a retrospective study from the European Group for blood and marrow transplantation. Bone Marrow Transpl. 2013;48:809–13.
Zhou X, Zhang S, Fan J, Zhu X, Hu S. Risk factors for BK virus-associated hemorrhagic cystitis after allogeneic hematopoietic stem cell transplantation: a systematic review and meta-analysis. Clin Transpl. 2023:e15121.
Riachy E, Krauel L, Rich BS, McEvoy MP, Honeyman JN, Boulad F, et al. Risk factors and predictors of severity score and complications of pediatric hemorrhagic cystitis. J Urol. 2014;191:186–92.
Lam W, Storek J, Li H, Geddes M, Daly A. Incidence and risk factor of hemorrhagic cystitis after allogeneic transplantation with fludarabine, busulfan, and anti-thymocyte globulin myeloablative conditioning. Transpl Infect Dis. 2017;19.
Heath JA, Mishra S, Mitchell S, Waters KD, Tiedemann K. Estrogen as treatment of hemorrhagic cystitis in children and adolescents undergoing bone marrow transplantation. Bone Marrow Transpl. 2006;37:523–6.
Bedi A, Miller CB, Hanson JL, Goodman S, Ambinder RF, Charache P, et al. Association of BK virus with failure of prophylaxis against hemorrhagic cystitis following bone marrow transplantation. J Clin Oncol. 1995;13:1103–9.
Cesaro S, Facchin C, Tridello G, Messina C, Calore E, Biasolo MA, et al. A prospective study of BK-virus-associated haemorrhagic cystitis in paediatric patients undergoing allogeneic haematopoietic stem cell transplantation. Bone Marrow Transpl. 2008;41:363–70.
Jiang H, Geng L, Wan X, Song K, Tong J, Zhu X, et al. Incidence and risk factors of late-onset hemorrhagic cystitis after single umbilical cord blood transplantation with myeloablative conditioning regimen. Int J Hematol. 2021;114:381–9.
Salamonowicz-Bodzioch M, Frączkiewicz J, Czyżewski K, Zając-Spychała O, Gorczyńska E, Panasiuk A, et al. Prospective analysis of BKV hemorrhagic cystitis in children and adolescents undergoing hematopoietic cell transplantation. Ann Hematol. 2021;100:1283–93.
Hassan Z, Remberger M, Svenberg P, Elbander M, Omazic B, Mattsson J, et al. Hemorrhagic cystitis: a retrospective single-center survey. Clin Transpl. 2007;21:659–67.
Saito Y, Kumamoto T, Shiraiwa M, Sonoda T, Arakawa A, Hashimoto H, et al. Cyclophosphamide-induced hemorrhagic cystitis in young patients with solid tumors: a single institution study. Asia Pac J Clin Oncol. 2018;14:e460–4.
Kaur D, Khan SP, Rodriguez V, Arndt C, Claus P. Hyperbaric oxygen as a treatment modality in cyclophosphamide-induced hemorrhagic cystitis. Pediatr Transpl. 2018;22:e13171.
Pode D, Perlberg S, Steiner D. Busulfan-induced hemorrhagic cystitis. J Urol. 1983;130:347–8.
Santos Carreira A, Salas MQ, Remberger M, Novitzky-Basso I, Law AD, Lam W, et al. Interaction between High-Dose Intravenous Busulfan and Post-transplantation Cyclophosphamide on Hemorrhagic Cystitis after allogeneic hematopoietic cell transplantation. Transpl Cell Ther. 2023;29:e5811–8.
Fu H, Xu L, Liu D, Zhang X, Liu K, Chen H, et al. Late-onset hemorrhagic cystitis after haploidentical hematopoietic stem cell transplantation in patients with advanced leukemia: differences in ATG dosage are key. Int J Hematol. 2013;98:89–95.
Hess NJ, Brown ME, Capitini CM. GVHD Pathogenesis, Prevention and Treatment: lessons from Humanized Mouse Transplant models. Front Immunol. 2021;12:723544.
Ost L, Lönnqvist B, Eriksson L, Ljungman P, Ringdén O. Hemorrhagic cystitis–a manifestation of graft versus host disease? Bone Marrow Transpl. 1987;2:19–25.
Zhang L, Khadka B, Wu J, Feng Y, Long B, Xiao R, et al. CMV infection is a risk factor for hemorrhagic cystitis after hematopoietic stem cell transplantation. Ann Hematol. 2023;102:1193–201.
Bennett SM, Broekema NM, Imperiale MJ. BK Polyomavirus: emerging pathogen. Microbes Infect. 2012;14:672–83.
Leung AY, Chan MT, Yuen KY, Cheng VC, Chan KH, Wong CL, et al. Ciprofloxacin decreased polyoma BK virus load in patients who underwent allogeneic hematopoietic stem cell transplantation. Clin Infect Dis. 2005;40:528–37.
Liu P, Bai K, Zhang Z, Sun J. Analysis of early clinical signs and risk factors for severe hemorrhagic cystitis after stem cell transplantation in children. Int J Urol. 2024.
Jepsen C, Turkiewicz D, Ifversen M, Heilmann C, Toporski J, Dykes J, et al. Low incidence of hemorrhagic cystitis following ex vivo T-cell depleted haploidentical hematopoietic cell transplantation in children. Bone Marrow Transpl. 2020;55:207–14.
Kwon HJ, Kang JH, Lee JW, Chung NG, Kim HK, Cho B. Treatment of BK virus-associated hemorrhagic cystitis in pediatric hematopoietic stem cell transplant recipients with cidofovir: a single-center experience. Transpl Infect Dis. 2013;15:569–74.
Cesaro S, Hirsch HH, Faraci M, Owoc-Lempach J, Beltrame A, Tendas A, et al. Cidofovir for BK virus-associated hemorrhagic cystitis: a retrospective study. Clin Infect Dis. 2009;49:233–40.
Wu KH, Weng T, Wu HP, Peng CT, Sheu JN, Chao YH. Effective treatment of severe BK virus-associated hemorrhagic cystitis with leflunomide in children after hematopoietic stem cell transplantation: a pilot study. Pediatr Infect Dis J. 2014;33:1193–5.
Park YH, Lim JH, Yi HG, Lee MH, Kim CS. BK virus-hemorrhagic cystitis following allogeneic stem cell transplantation: clinical characteristics and utility of leflunomide treatment. Turk J Haematol. 2016;33:223–30.
Rubinstein JD, Zhu X, Leemhuis T, Pham G, Ray L, Emberesh S, et al. Virus-specific T cells for adenovirus infection after stem cell transplantation are highly effective and class II HLA restricted. Blood Adv. 2021;5:3309–21.
Leen AM, Bollard CM, Mendizabal AM, Shpall EJ, Szabolcs P, Antin JH, et al. Multicenter study of banked third-party virus-specific T cells to treat severe viral infections after hematopoietic stem cell transplantation. Blood. 2013;121:5113–23.
Tzannou I, Papadopoulou A, Naik S, Leung K, Martinez CA, Ramos CA, et al. Off-the-Shelf Virus-Specific T cells to treat BK Virus, Human Herpesvirus 6, Cytomegalovirus, Epstein-Barr Virus, and Adenovirus infections after allogeneic hematopoietic stem-cell transplantation. J Clin Oncol. 2017;35:3547–57.
Olson A, Lin R, Marin D, Rafei H, Bdaiwi MH, Thall PF, et al. Third-Party BK Virus-Specific cytotoxic T lymphocyte therapy for Hemorrhagic Cystitis following Allotransplantation. J Clin Oncol. 2021;39:2710–19.
Acknowledgements
We would like to thank Editage (www.editage.cn) for English language editing.
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The work was supported by the following grants: National Key R&D Program of China (2022YFC2502700), National Natural Science Foundation of China (No. 82170218 and 82300244), Jiangsu key project (No. BE2021654), Suzhou project (No. SZS2023014, SZS201615, SKY2022012), Soochow University Program (ML13101223), Children’s Hospital of Soochow Program (2023SYLCYJ01) and the Suzhou Clinical Trial Institution Capacity Enhancement Program, SLT2021003.
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Senlin Zhang, Minyuan Liu, and Qingwei Wang: literature search, statistical analysis, data extraction, interpretation of the data, drafting and revision of the manuscript. Shuran Wang, Xin Liu, Bohan Li, and Jie Li: statistical analysis, data extraction. Junjie Fan and Shaoyan Hu: study supervision and manuscript revision.
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Zhang, S., Liu, M., Wang, Q. et al. Risk factors for hemorrhagic cystitis in children undergoing hematopoietic stem cell transplantation: a systematic review and meta-analysis. BMC Pediatr 24, 333 (2024). https://doi.org/10.1186/s12887-024-04815-x
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DOI: https://doi.org/10.1186/s12887-024-04815-x