Analytical pipeline captured in the provided ACS example file. The Figure shows an overview of the analytical pipeline captured in the ACS example file. The work flow is demonstrated by solid line arrows. Data files are preprocessed and manually gated for monocytes, dendritic cells (DCs, including myeloid and plasmacytoid subpopulations - mDCs and pDCs) and B cells, as well as additional novel populations whose roles in the immune response still need to be further investigated. The gating hierarchy (stored in Gating-ML files) is indicated by dashed line arrows. In parallel to this effort, the preprocessed FCS data files are fed to a clustering and cluster matching algorithm, which identifies monocytes, dendritic cells, B cells and additional subpopulations independently of the manual gating. The results of the automated and manual gating process are compared in order to determine the suitability of this automated analysis approach.
Spidlen et al. BMC Research Notes 2011 4:50 doi:10.1186/1756-0500-4-50