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Open Access Data Note

Collection of Macaca fascicularis cDNAs derived from bone marrow, kidney, liver, pancreas, spleen, and thymus

Naoki Osada1*, Makoto Hirata1, Reiko Tanuma1, Yutaka Suzuki2, Sumio Sugano2, Keiji Terao3, Jun Kusuda1, Yosuke Kameoka1, Katsuyuki Hashimoto1 and Ichiro Takahashi1

Author Affiliations

1 Department of Biomedical Resources, National Institute of Biomedical Innovation, 7-6-8 Saito-Asagi, Ibaraki, Osaka 567-0085, Japan

2 Department of Medical Genome Sciences, Graduate School of Frontier Sciences, University of Tokyo, 5-1-5 Kashiwano-ha, Kashiwa, Chiba 277-8561. Japan

3 Tsukuba Primate Research Center, National Institute of Biomedical Innovation, 1 Hachimandai, Tsukuba 305-0843, Japan

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BMC Research Notes 2009, 2:199  doi:10.1186/1756-0500-2-199

Published: 29 September 2009

Abstract

Background

Consolidating transcriptome data of non-human primates is essential to annotate primate genome sequences, and will facilitate research using non-human primates in the genomic era. Macaca fascicularis is a macaque monkey that is commonly used for biomedical and ecological research.

Findings

We constructed cDNA libraries of Macaca fascicularis, derived from tissues obtained from bone marrow, liver, pancreas, spleen, and thymus of a young male, and kidney of a young female. In total, 5'-end sequences of 56,856 clones were determined. Including the previously established cDNA libraries from brain and testis, we have isolated 112,587 cDNAs of Macaca fascicularis, which correspond to 56% of the curated human reference genes.

Conclusion

These sequences were deposited in the public sequence database as well as in-house macaque genome database http://genebank.nibio.go.jp/qfbase/ webcite. These data will become valuable resources for identifying functional parts of the genome of macaque monkeys in future studies.