Table 1

Differentially expressed proteins in mitochondria of metastatic 5-8F compared to non-metastatic 6-10B cells, identified by 2D-DIGE and MS (Ratio = 5-8F/6-10B; +, up-regulated; -, down-regulated)
Proteins names Spots no. Accession number Gene names Subcellular location Mass (Da) pI Mascot score NMP NUP MOI Sequence coverage (%) Average ratio
1. Oxidoreductase Proteins
Peroxiredoxin 3 2 gi|119569781 PRDX3 Mitochondria 24,941 5.73 90 5 9 PMF 43 +2.2
Peroxiredoxin 6 10 gi|4758638 PRDX6 Mitochondria, lysosome 25,133 8.36 71 5 8 PMF 21 +2.5
Superoxide dismutase 13 gi|67782309 SOD2 Mitochondria 20,882 8.36 71 4 4 PMF 52 +3.3
[Mn], mitochondrial isoform B precursor 62 2 \ MS/MS 8
Isocitrate dehydrogenase 12 gi|5031777 IDH3B Mitochondria 40,022 6.47 75 6 10 PMF 15 -3.3
[NAD] subunit alpha, mitochondrial precursor 73 1 \ MS/MS 3
Protein disulfide isomerase related protein 5 5 gi|1710248 PDIA5 Endoplasmic reticulum 46,512 4.95 166 9 4 PMF 37 +2.4
Cytochrome c oxidase subunit 5A, mitochondrial precursor 9 gi|190885499 COX5A Mitochondria 16,923 6.30 72 4 3 PMF 38 -4.4
Electron transfer 6 gi|2781202 ETFA Mitochondria 33,418 6.95 73 4 2 PMF 27 +2.6
flavoprotein 95 3 \ MS/MS 11
2. Porin Proteins
Volatage-dependent anion selective channel protein 1 4 gi|4507879 VDAC1 Mitochondria 30,868 8.62 117 7 8 PMF 38 +2.4
Volatage-dependent anion channel 2 1 gi|55664661 VDAC2 Mitochondria 30,842 8.00 101 6 5 PMF 34 +2.2
3. Metabolism and regulation Proteins
Delta(3.5)-Delta(2.4)-dienovl-CoA isomerase, mitochondrial precursor 11 gi|70995211 ECH1 Mitochondria 36,136 8.16 105 7 10 PMF 44 +4.1
Prohibitin 7 gi|4505773 PHB Mitochondria 29.843 5.57 158 8 1 PMF 44 +2.0
Maspin 15 gi|56554671 SERPINB5 Extracellular region 43,690 5.99 225 5 \ MS/MS 15 -3.2
Eukaryotic initiation factor 5A 14 gi|4261795 EIF5A Endoplasmic reticulum 17,018 4.85 68 3 \ MS/MS 15 +3.2
26S protease regulatory subunit 7 isoform 1 3 gi|4506209 PSMC4 Mitochondria, proteasome 49,002 5.71 92 6 2 PMF 19 +2.0
Ribosomal protein L7/L12, mitochondria 8 gi|1313962 MRPL12 Mitochindria 21,593 9.04 86 4 \ MS/MS 19 -3.9
Calumenin isoform c precursor 16 gi|314122177 CALU Endoplasmic reticulum 38,141 4.47 124 8 9 PMF 31 +2.9

NMP, the number of matched peptides. NUP, the number of unmatched peptides. MOI, methods of identification.

Liu et al.

Liu et al. BMC Medical Genomics 2012 5:62   doi:10.1186/1755-8794-5-62

Open Data