Up-regulated expressions of PRDX3 in metastatic 5-8F cell mitochondria relative to nonmetastatic 6-10B cell mitochondria without significant expression difference in the level of whole cells.A. Western blotting image. B. The quantitative comparison of the digitized Western blotting image. COXIV, a marker protein of mitochondria, was used as a reference standard protein when mitochondrial protein sample was analyzed with Western blot. ACTIN that is mainly expressed in the cytoplasm was used a reference standard protein when whole-cell protein sample was analyzed with Western blot. Western blot experiments were carried out triply (n = 3).
Liu et al. BMC Medical Genomics 2012 5:62 doi:10.1186/1755-8794-5-62