Figure 5 .
Real-time quantitative PCR analysis and Western blot confirmation of the differentially expressed proteins. ( A) The mRNA expression levels of 11 differentially expressed proteins in 2-DE gels were analyzed by qRT-PCR. GAPDH was used as the internal control. Upregulation of PIG3, P53 ( B) and downregulation of ANAX2 ( C) in HCT116 gankyrin−/− cells was confirmed by Western blot analysis.
Luo et al. BMC Medical Genomics 2012 5:36 doi:10.1186/1755-8794-5-36