Email updates

Keep up to date with the latest news and content from BMC Medical Genomics and BioMed Central.

Open Access Research article

Gastric cancers of Western European and African patients show different patterns of genomic instability

Tineke E Buffart1, Melanie Louw2, Nicole CT van Grieken1, Marianne Tijssen1, Beatriz Carvalho1, Bauke Ylstra1, Heike Grabsch3, Chris JJ Mulder4, Cornelis JH van de Velde5, Schalk W van der Merwe6 and Gerrit A Meijer1*

Author Affiliations

1 Dept. of Pathology, VU University Medical Center, Amsterdam, The Netherlands

2 Dept. of Anatomical Pathology, University of Pretoria, Pretoria, South Africa

3 Pathology and Tumour Biology, Leeds Institute of Molecular Medicine, University of Leeds, Leeds, UK

4 Dept. of Gastroenterology, VU University Medical Center Amsterdam, The Netherlands

5 Dept. of Surgery, Leiden University Medical Center, Leiden, The Netherlands

6 Dept of Internal Medicine and Gastroenterology, University of Pretoria, South Africa

For all author emails, please log on.

BMC Medical Genomics 2011, 4:7  doi:10.1186/1755-8794-4-7

Published: 13 January 2011

Abstract

Background

Infection with H. pylori is important in the etiology of gastric cancer. Gastric cancer is infrequent in Africa, despite high frequencies of H. pylori infection, referred to as the African enigma. Variation in environmental and host factors influencing gastric cancer risk between different populations have been reported but little is known about the biological differences between gastric cancers from different geographic locations. We aim to study genomic instability patterns of gastric cancers obtained from patients from United Kingdom (UK) and South Africa (SA), in an attempt to support the African enigma hypothesis at the biological level.

Methods

DNA was isolated from 67 gastric adenocarcinomas, 33 UK patients, 9 Caucasian SA patients and 25 native SA patients. Microsatellite instability and chromosomal instability were analyzed by PCR and microarray comparative genomic hybridization, respectively. Data was analyzed by supervised univariate and multivariate analyses as well as unsupervised hierarchical cluster analysis.

Results

Tumors from Caucasian and native SA patients showed significantly more microsatellite instable tumors (p < 0.05). For the microsatellite stable tumors, geographical origin of the patients correlated with cluster membership, derived from unsupervised hierarchical cluster analysis (p = 0.001). Several chromosomal alterations showed significantly different frequencies in tumors from UK patients and native SA patients, but not between UK and Caucasian SA patients and between native and Caucasian SA patients.

Conclusions

Gastric cancers from SA and UK patients show differences in genetic instability patterns, indicating possible different biological mechanisms in patients from different geographical origin. This is of future clinical relevance for stratification of gastric cancer therapy.