Additional file 6.

Urinary metabolic-profiling analysis of experiment II (repeated low-calcium diet experiment). (A) Principal component analysis (PCA) score plots of the low-calcium group (LCG; black triangles) and normal-calcium group (NCG; red circles) rats. (n = 24, week 1 to 12). Each data point represents one subject. (B) Partial least-squares discriminant analysis (PLS-DA) score plot between LCG (black triangles) and NCG (red circles). (n = 24, week 1 to 12). Each data point represents one subject. (C) Permutation test result of PLS-DA model. The R2Y value represents the goodness of fit of the model, and the Q2 value represents the predictability of the models. (D) Batch PLS score plot of urine samples mapped against time (n = 24, week 1 to 12). Dashed horizontal lines show two and three standard deviations for the dataset. LCG rats are shown as black lines and NCG rats as red lines. (E) PCA scores plots of LCG (black squares) and NCG (red triangles) rats. (A-C) Week 2, n = 24 (for two components R2Y = 48.8%, Q2 = 20.7%); week 4, n = 24 (for three components R2Y = 55.1%, Q2 = 26.4%) and week 9, n = 24 (for three components R2Y = 59.1%, Q2 = 28.7%). Each data point represents one subject. (F) Metabolic trajectory scores plots by PCA model derived from the urine of LCG rats (n = 12) from weeks 1 to 12. Black triangle: weeks 1 to 3; red star: weeks 4 to 8; blue circle: weeks 9 to 12. Comp, component. t[1], component 1; t[2], component 2.

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Wang et al. BMC Medicine 2013 11:86   doi:10.1186/1741-7015-11-86