The GLK expression levels in T cells from patients with adult-onset Still's diseases and healthy controls. Representative examples of flow cytometry contour plots of intracellular GLK production in CD3+ T-cells, CD4+ T-cells, and CD8+ T-cells were obtained from peripheral blood of (A) one patient with AOSD and (B) one healthy control. (C) The frequencies of circulating GLK-expressing CD3+ T-cells were obtained from 24 patients with active AOSD and 12 HC. (D) The comparison in the relative expression levels of GLK transcript between patients with AOSD and HC. (E) Immunoblot analyses of GLK expression in the lysates of peripheral blood T cells from patients with AOSD and HC. (F) The comparison in the relative expression levels of GLK protein between patients with active AOSD and HC. Horizontal bar indicates median value. *P-value was determined by Mann-Whitney U test. AOSD: adult-onset Still's disease; GLK: germinal center kinase-like kinase; HC: healthy control.
Chen et al. BMC Medicine 2012 10:84 doi:10.1186/1741-7015-10-84