Table 1

Properties of mitochondria and their genomes.

Property

Value


Mitochondria

1. total length/cell (μm)

600 ± 124a

mt DNA foci

2. lineal density in mitochondria (foci/μm)

0.78b, 0.82c

3. foci/cell

468 ± 97d, 492 ± 102e

4. diameter (range; nm)

70f, 65 (31–132)g

mtDNA

5. molecules/cell

3,500h

6. molecules/focus

7.5i, 7.1j, 9.2k, 5.8l, 10m


ausing stacks of images of 50 cells (one image from a stack is shown in Figure 1A). busing anti-DNA as in Figure 1A,1B; 43 cells analyzed. cusing anti-BrdU as in Figure 1C,1D; 38 cells analyzed. dfrom rows 1 and 2 (anti-DNA). efrom rows 1 and 2 (anti-BrdU). ffrom 120 clusters of gold particles like that in Figure 2A, after correcting for sectioning effects). gfrom 97 clusters of gold particles like that in Figure 2B. hfrom gel electrophoresis; value similar to that found by Jacobs et al. [14]. ifrom rows 3 (anti-DNA) and 5. jfrom rows 3 (anti-BrdU) and 5. kfrom mean intensity in Figure 1F, assuming the faintest focus contains 1 molecule. lfrom row 4 (unextracted cells), assuming DNA of 16.5 kbp is packed at 35 mg/ml (that is, the density of the bacterial nucleoid) [26] into a sphere (diameter 68 nm). mfrom replication pattern (Figure 6).

Iborra et al. BMC Biology 2004 2:9   doi:10.1186/1741-7007-2-9

Open Data