Additional file 3.

Quantification of polar metabolites of Plasmodium falciparum-infected and uninfected red blood cells (RBCs) (related to Figure 3A). Ring-infected RBCs, gametocytes, and uninfected RBCs were cultured in the presence or absence of NaFAc, and the metabolic and morphological effects were assessed. (A) After culturing in the presence or absence of 1 mmol sodium fluoracetate (NaFAc), metabolites were extracted from schizont-infected and uninfected RBCs (at 38 hours) and gametocytes (at 24 hours), and polar metabolites of interest were quantified by gas chromatography–mass spectrometry (GC-MS) and comparison with known standards. Abundances are shown in nmoles per 108 cells, with numbers in brackets representing standard deviation, where n = 4 for gametocyte analyses, and n = 3 for asexual stage analyses. The abundance ratio of cells cultured in the presence of NaFAc to the absence of NaFAc is also shown. All values are to 2 d.p. Abbreviations are described in Figures 1 and 3.

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MacRae et al. BMC Biology 2013 11:67   doi:10.1186/1741-7007-11-67