Additional file 1.

Gas chromatography–mass spectrometry (GC-MS) chromatograms of Plasmodium falciparum-infected and uninfected red blood cell (RBC) polar metabolites. Gametocytes (top row), schizont-infected RBCs (asexuals, middle row), and uninfected RBCs (bottom row) were harvested, and metabolites extracted as described. The panels depict representative total ion chromatograms (TICs) and extracted ion chromatograms (EICs) of metabolites extracted from 108 cells. Panels in the left-hand column show full TICs. The area of the chromatogram containing the tricarboxylic acid (TCA) metabolites (purple box) is shown in detail in the panels of the middle column. Quantification was performed as described, using extracted ions to distinguish between overlapping peaks. An example is shown in the EIC panels in the right-hand column, depicting the area highlighted by the orange box in the TICs. Lines represent the monoisotopic (diagnostic) ion for aspartic acid (green), glutamine or glutamic acid (blue), and γ-aminobutyric acid (GABA; red) (note the difference in the y-axes). Abundances are shown in arbitrary units, with absolute quantifications shown in Additional file 3. NB: Quantification for glutamic acid was performed using the peak (E) corresponding to glutamate alone. Abbreviations: C, citrate; D, aspartate; E, glutamate; F, fumarate; G, GABA; M, malate; S, succinate; X (Gln/Glu), glutamine/glutamate.

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MacRae et al. BMC Biology 2013 11:67   doi:10.1186/1741-7007-11-67