Characterization of the colonic epithelial response in B. thetaiotaomicron and F. prausnitzii di-associated rats. (A) Measurement of colonic crypt depth, total number of cells per colonic crypt and counts of (B) alcian blue- (indicated as AB) and periodic acid Schiff- (indicated as PAS) positive cells per crypt in colonic sections of Bt-30d (n = 7) and Bt + Fp-30d rats (n = 6). (C) Representative Western blot and densitometric analyses of proteins involved in the differentiation pathway of the secretory lineage, KLF4 and Chromogranin A (ChgA) in Bt-30d (n = 5) and Bt + Fp-30d rats (n = 4); protein fold induction in Bt-30d rats was used as a reference and arbitrarily defined as 1. (D) Immunostaining for MUC2 in germfree (GF) (n = 3), Bt-2d (n = 6), Bt-30d (n = 7) and Bt + Fp-30d rats (n = 6); scale bars, 50 μm. The asterisk indicates a statistical difference compared to Bt-30d rats (P-value <0.05); n.s., not significant.
Wrzosek et al. BMC Biology 2013 11:61 doi:10.1186/1741-7007-11-61