Characterization of the colonic epithelial response in B. thetaiotaomicron mono-associated rats. (A) Colonic crypt depth and total number of cells per crypt were determined on sections from GF (n = 3), Bt-2d (n = 5) and Bt-30d rats (n = 7) stained with Haematoxylin-Eosin-Safran (HES). (B, C) Representative pictures and graphs showing counts of goblet cells staining with (B) alcian blue (indicated as AB) and (C) periodic acid Schiff (indicated as PAS) in GF (n = 3), Bt-2d (n = 6) and Bt-30d (n = 7) samples. Scale bars, 50 μm. (D) Representative Western blots and densitometric analyses of proteins involved in the differentiation pathway of the secretory lineage, KLF4 and Chromogranin A (ChgA), in GF (n = 7), Bt-2d (n = 6) and Bt-30d (n = 7) samples. Means with different letters are significantly different (P-value <0.05).
Wrzosek et al. BMC Biology 2013 11:61 doi:10.1186/1741-7007-11-61