Time course analysis of GIT in B. thetaiotaomicron mono-associated rats. Germfree (GF) rats were inoculated with a culture of B. thetaiotaomicron (107 CFU) and euthanized 2 days (Bt-2d) or 30 days (Bt-30d) after inoculation. (A) Establishment of B. thetaiotaomicron in the gastrointestinal tract (GIT) of Bt-30d rats (n = 13) was monitored weekly by enumeration of the bacterial counts in the feces. (B) Scanning electron microscopy images of B. thetaiotaomicron in the cecum of Bt-2d and Bt-30d rats; scale bars, 1 μm. (C) Measurement of cecal pH in GF (n = 12), Bt-2d (n = 13) and Bt-30d rats (n = 19). (D) Cecal concentration of short-chain fatty acids (SCFA) in GF (n = 16), Bt-2d (n = 13) and Bt-30d rats (n = 19); only results for acetate, propionate and butyrate are shown, other SCFA were not detected; results are expressed in mM. Means with different letters are significantly different (P-value <0.05).
Wrzosek et al. BMC Biology 2013 11:61 doi:10.1186/1741-7007-11-61