Table 1

E. coli strains

Strains

Genotype/Relevant characteristics

Source


OP50

OP50 ura

[37]

RNAi control strain

HT115(DE3) W3110 rnc14::Tn10 (DE3 PlacUV5-T7 polymerase)

[46]

aroD mutant

HT115(DE3) aroD717::IS1

This study

Keio collection WT

BW25113

[16]

Keio collection aroD

BW25113 aroD

[16,36], This study

OP50 Su resistant

OP50 R26

This study; [47]

aroD mutant/ugt-27 RNAi

HT115(DE3)aroD717::IS1/L4440(ugt-27)

This study; [38]

RNAi control + vector

HT115(DE3)/pMMB67EH

This study

aroD mutant + vector

HT115(DE3)aroD717::IS1/pMMB67EH

This study

mutant + aroD

HT115(DE3)aroD717::IS1/pMMB67EH[aroD]

This study

ubiG mutant

GD1 ubiG

[15]

ubiG mutant + rescue

GD1 ubiG/pAHG(ubiG+)

[15]


Virk et al. BMC Biology 2012 10:67   doi:10.1186/1741-7007-10-67

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