Additional file 9.

Scatter plots of the concentration inhibiting cell viability by 50% (GI50) values for cell viability studies summarized in Table 2. (a-c) U-87 MG, KNS42, LICR-LON-HN4 and MDA-MB-231 (P and M) cells were grown in two dimensions (monolayer) and in three dimensions (spheroids). Then, 4 days later, cells were treated with (a) 17-N-allylamino-17-demethoxygeldanamycin (17-AAG) (final concentrations 0 to 100 μM for U-87 MG and KNS42, 0 to 25 μM for MDA-MB-231 P and M, 0 to 1 μM for LICR-LON-HN4), (b) PI-103 (final concentrations 0 to 25 μM for U-87 MG and KNS42) and (c) CCT130234 (final concentrations 0 to 100 μM for U-87 MG and MDA-MB-231 P). Then, 72 h later, a CellTiter Glo luminescence assay for cell viability was performed and GI50 values were determined. Dots represent GI50 values from each assay and means ± SD are plotted.

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Vinci et al. BMC Biology 2012 10:29   doi:10.1186/1741-7007-10-29