Additional file 2.

Generation of tumor spheroids: comparative methods. U-87 MG spheroids were generated in (a) U-bottomed 96-well ultra-low attachment (ULA) plates, (b) flat-bottomed 96-well agar-coated plates, (c) 24-well poly-Hema-coated plates and (d) in a Rotary Cell Culture System (RCCS; Cellon). In (a) and (b) one spheroid/well was obtained with a tighter structure in the former. In (c) and (d) multiple variably-sized spheroids were formed. All the images were taken 4 days post initiation. Scale bar: 200 μm.

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Vinci et al. BMC Biology 2012 10:29   doi:10.1186/1741-7007-10-29