Additional file 14.

17-N-Allylamino-17-demethoxygeldanamycin (17-AAG) mediated concentration-dependent inhibition of tumor spheroid invasion into Matrigel. (a-d) day 4 U-87 MG or MDA-MB-231 M (metastatic variant) spheroids were embedded into Matrigel for three-dimensional invasion. 17-AAG was used over a range of concentrations and control spheroids were treated with vehicle. Images were captured at intervals from t0 to 72 h using a Celigo cytometer for U-87 MG or an inverted microscope for MDA-MB-231 M. The area of invasion was determined using Image-Pro Analyzer software. Representative examples of three separate experiments are shown for U-87 MG (a, b) and for MDA-MB-231 M (c, d). Values are means ± SD, n = 6. (b, d) Representative images of spheroid invasion were obtained on a Celigo cytometer (b) or by microscopy (d) at t0 and 24 h. Scale bar: 500 μm.

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Vinci et al. BMC Biology 2012 10:29   doi:10.1186/1741-7007-10-29