Additional file 13.
17-N-Allylamino-17-demethoxygeldanamycin (17-AAG) concentration-dependent inhibition of tumor spheroid growth kinetics: alternative non-automated analysis. (a) U-87 MG and (b) LICR-LON-HN4 spheroids were treated on day 4 with 17-AAG (0 to 2 μM for U-87 MG and 0 to 2.93 μM for LICR-LON-HN4), according to their three-dimensional concentration inhibiting cell viability by 50% (GI50) values (see Table 2). Images were obtained by microscopy as described above and analysis of spheroid growth was performed using Image-Pro Analyzer software. Graphs show a concentration-dependent growth inhibition of the spheroids from day 4 to day 14. Values are means ± SD, n = 6; one representative of three separate experiments is shown for each cell line.
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Vinci et al. BMC Biology 2012 10:29 doi:10.1186/1741-7007-10-29