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Overexpression of SERBP1 (Plasminogen activator inhibitor 1 RNA binding protein) in human breast cancer is correlated with favourable prognosis

Nuran Bektas Serce1, Andreas Boesl2, Irina Klaman3, Sonja von Serényi4, Erik Noetzel4, Michael F Press5, Arno Dimmler6, Arndt Hartmann7, Jalid Sehouli8, Ruth Knuechel4, Matthias W Beckmann9, Peter A Fasching109 and Edgar Dahl4*

Author Affiliations

1 Department of Pathology, University Hospital Bonn, Sigmund-Freud-Str. 25, 53127, Bonn, Germany

2 Department of Pathology, Vorarlberger Krankenhaus-Betriebsgesellschaft m.b.H. Hospital, Feldkirch, Austria

3 Signature Diagnostics AG, Voltaireweg 4b, 14469, Potsdam, Germany

4 Molecular Oncology Group, Institute of Pathology, University Hospital of the RWTH Aachen, Pauwelsstrasse 30, 52074, Aachen, Germany

5 Department of PathologyKeck School of Medicine, University of Southern California/Norris Comprehensive Cancer Center, 1441 Eastlake Avenue, Los Angeles, CA, 90033, U.S.A

6 Department of Pathology, St.-Vincentius-Kliniken, Südenstr. 37, 76137, Karlsruhe, Germany

7 Department of Pathology, University Hospital Erlangen, Krankenhausstrasse 12, 91054, Erlangen, Germany

8 Department of Gynaecology, Charité Campus Virchow-Klinikum, Augustenburger Platz 1, 13353, Berlin, Germany

9 Department of Gynaecology, University Hospital Erlangen, Universitätsstrasse 21-23, 91054, Erlangen, Germany

10 David Geffen School of Medicine, Division of Hematology and Oncology, University of California at Los Angeles, Los Angeles, USA

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BMC Cancer 2012, 12:597  doi:10.1186/1471-2407-12-597

Published: 13 December 2012

Additional files

Additional file 1:

Figure S1. Validation of SERBP1 antibody in Western blot analysis/Competition test. The SERBP1 antibody was validated in Western blot analysis by using the peptide competition test. SERBP1 protein expression was investigated in extracts of cryoconserved placenta, liver and in the breast cancer cell lines MDA-MB 231 and BT20. After peptide competition the SERBP1 protein signal was absent or weak whereas ß-actin displayed a homogenous protein loading.

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Additional file 2:

Table S1. Clinicopathological and immunohistochemical parameters in relation to SERBP1 immunoreactivity in the evaluation TMA.

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Additional file 3:

Table S2. Univariate analysis of factors regarding overall survival (OS) and recurrence-free survival (RFS) in the evaluation TMA.

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Additional file 4:

Figure S2. Correlation of SERBP1 expression and patient prognosis according to univariate Kaplan-Meier analysis. Breast cancer patients overexpressing SERBP1 presented favourable prognosis in recurrence-free survival analysis by trend (P = 0.301).

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