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Open Access Highly Accessed Technical advance

Rapid screening for chromosomal aneuploidies using array-MLPA

Jing-Bin Yan12, Miao Xu1, Can Xiong1, Da-Wen Zhou1, Zhao-Rui Ren1, Ying Huang12, Monique Mommersteeg3, Rinie van Beuningen3, Ying-Tai Wang4, Shi-Xiu Liao4, Fanyi Zeng125, Ying Wu13* and Yi-Tao Zeng12*

Author Affiliations

1 Institute of Medical Genetics, Children's Hospital of Shanghai, Shanghai Jiao Tong University, Shanghai, P.R. China

2 Key Lab of Embryo Molecular Biology, Ministry of Health, and Shanghai Lab of Embryo and Reproduction Engineering, Shanghai, P.R. China

3 PamGene International BV, 's-Hertogenbosch, The Netherlands

4 Medical Genetic Institute of Henan Province, the People's Hospital of Henan Province, Zhengzhou, P.R. China

5 Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai, P.R. China

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BMC Medical Genetics 2011, 12:68  doi:10.1186/1471-2350-12-68

Published: 17 May 2011

Abstract

Background

Chromosome abnormalities, especially trisomy of chromosome 21, 13, or 18 as well as sex chromosome aneuploidy, are a well-established cause of pregnancy loss. Cultured cell karyotype analysis and FISH have been considered reliable detectors of fetal abnormality. However, results are usually not available for 3-4 days or more. Multiplex ligation-dependent probe amplification (MLPA) has emerged as an alternative rapid technique for detection of chromosome aneuploidies. However, conventional MLPA does not allow for relative quantification of more than 50 different target sequences in one reaction and does not detect mosaic trisomy. A multiplexed MLPA with more sensitive detection would be useful for fetal genetic screening.

Methods

We developed a method of array-based MLPA to rapidly screen for common aneuploidies. We designed 116 universal tag-probes covering chromosomes 13, 18, 21, X, and Y, and 8 control autosomal genes. We performed MLPA and hybridized the products on a 4-well flow-through microarray system. We determined chromosome copy numbers by analyzing the relative signals of the chromosome-specific probes.

Results

In a blind study of 161 peripheral blood and 12 amniotic fluid samples previously karyotyped, 169 of 173 (97.7%) including all the amniotic fluid samples were correctly identified by array-MLPA. Furthermore, we detected two chromosome X monosomy mosaic cases in which the mosaism rates estimated by array-MLPA were basically consistent with the results from karyotyping. Additionally, we identified five Y chromosome abnormalities in which G-banding could not distinguish their origins for four of the five cases.

Conclusions

Our study demonstrates the successful application and strong potential of array-MLPA in clinical diagnosis and prenatal testing for rapid and sensitive chromosomal aneuploidy screening. Furthermore, we have developed a simple and rapid procedure for screening copy numbers on chromosomes 13, 18, 21, X, and Y using array-MLPA.