Table 1

Current (12/01/04) statistics for Floral Genome Project cDNA libraries, EST sequences and unigene builds. We will perform 10,000 EST sequencing reactions for each taxon listed in the top portion of the table and 2000 ESTs for each taxon in the bottom portion of the table.

Taxon

Primary Titre (pfu)

Amplified Titre (pfu/ml)

Avg Insert size (bp)

ESTs to date

aUnigenes

bObserved Redundancy


Libraries for deep sequencing

Welwitschia mirabilis (m) – Gymnosperm

3.25E+05

6.00E+08

1382

3732

2771

34.7%

Amborella trichopoda (m) – Basal angiosperm

2.24E+06

1.37E+10

1611

4047

6099

41.5%

Amborella trichopoda (f)

4.98E+06

1.40E+10

1031

4442

Nuphar advena (Water lily, Spadderdock) – Basal angiosperm

2.00E+06

3.20E+10

1134

8442

6205

36.1%

Acorus americanus (Sweet flag) – Basal monocot.

2.80E+06

6.00E+09

1083

5883

3067

28.8%

cAsparagus officinalis (m) – Transformable nongrass monocot

1.50E+06

1.20E+10

1468

5188

4560

61.5%

Asparagus officinalis (f)

1.30E+06

1.40E+10

1200

2174

Persea americana (Avocado) – Cultivated magnoliid

2.74E+06

2.57E+10

1349

8735

5314

41.3%

Liriodendron tulipifera (Tulip Poplar, Yellow Poplar) – Transformable lumber species

3.00E+06

2.00E+10

1346

9531

6520

46.2%

Saruma henryi (Upright Wild Ginger) – member of Aristolochiaceae with bipartite perianth

1.97E+06

1.67E+10

1587

3230

2631

21.8%

Eschscholzia californica (California poppy) – Transformable basal eudicot

7.00E+06

1.68E+11

1702

9079

6015

46.18%

Libraries for shallow sequencing

dCucumis sativus (m) (Cucumber) – rosid

------

------

------

1107

1648

23.5%

dCucumis sativus (f)

------

------

------

928

Ribes americanum (Black currant)

2.58E+06

2.25E+10

1200

2238

1791

25.0%

eVaccinium corymbosum (Blueberry) basal asterid

------

------

------

1758

1549

13.5%

Total:

70,514

48,170


m indicates library constructed from male tissues; f for female tissues.

aUnigene numbers are shown in the first line for taxa with multiple libraries.

bObserved redundancy was measured for each taxon as (EST# – Unigene #)/Unigene#.

c5188 ESTs from the male Asparagus library were sequenced in collaboration with Mike Havey (University of Wisconsin) and Chris Town (TIGR).

dMale and female Cucumis flower bud libraries described by Perl-Treves et al [71]

eVaccinium young inflorescence library was provided by Jeannine Rowland (USDA) [72].

Albert et al. BMC Plant Biology 2005 5:5   doi:10.1186/1471-2229-5-5

Open Data