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Current (12/01/04) statistics for Floral Genome Project cDNA libraries, EST sequences and unigene builds. We will perform 10,000 EST sequencing reactions for each taxon listed in the top portion of the table and 2000 ESTs for each taxon in the bottom portion of the table. |
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| Taxon |
Primary Titre (pfu) |
Amplified Titre (pfu/ml) |
Avg Insert size (bp) |
ESTs to date |
aUnigenes |
bObserved Redundancy |
|
|
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| Libraries for deep sequencing |
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| Welwitschia mirabilis (m) – Gymnosperm |
3.25E+05 |
6.00E+08 |
1382 |
3732 |
2771 |
34.7% |
| Amborella trichopoda (m) – Basal angiosperm |
2.24E+06 |
1.37E+10 |
1611 |
4047 |
6099 |
41.5% |
| Amborella trichopoda (f) |
4.98E+06 |
1.40E+10 |
1031 |
4442 |
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| Nuphar advena (Water lily, Spadderdock) – Basal angiosperm |
2.00E+06 |
3.20E+10 |
1134 |
8442 |
6205 |
36.1% |
| Acorus americanus (Sweet flag) – Basal monocot. |
2.80E+06 |
6.00E+09 |
1083 |
5883 |
3067 |
28.8% |
| cAsparagus officinalis (m) – Transformable nongrass monocot |
1.50E+06 |
1.20E+10 |
1468 |
5188 |
4560 |
61.5% |
| Asparagus officinalis (f) |
1.30E+06 |
1.40E+10 |
1200 |
2174 |
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| Persea americana (Avocado) – Cultivated magnoliid |
2.74E+06 |
2.57E+10 |
1349 |
8735 |
5314 |
41.3% |
| Liriodendron tulipifera (Tulip Poplar, Yellow Poplar) – Transformable lumber species |
3.00E+06 |
2.00E+10 |
1346 |
9531 |
6520 |
46.2% |
| Saruma henryi (Upright Wild Ginger) – member of Aristolochiaceae with bipartite perianth |
1.97E+06 |
1.67E+10 |
1587 |
3230 |
2631 |
21.8% |
| Eschscholzia californica (California poppy) – Transformable basal eudicot |
7.00E+06 |
1.68E+11 |
1702 |
9079 |
6015 |
46.18% |
| Libraries for shallow sequencing |
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| dCucumis sativus (m) (Cucumber) – rosid |
------ |
------ |
------ |
1107 |
1648 |
23.5% |
| dCucumis sativus (f) |
------ |
------ |
------ |
928 |
||
| Ribes americanum (Black currant) |
2.58E+06 |
2.25E+10 |
1200 |
2238 |
1791 |
25.0% |
| eVaccinium corymbosum (Blueberry) – basal asterid |
------ |
------ |
------ |
1758 |
1549 |
13.5% |
| Total: |
70,514 |
48,170 |
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|
m indicates library constructed from male tissues; f for female tissues. aUnigene numbers are shown in the first line for taxa with multiple libraries. bObserved redundancy was measured for each taxon as (EST# – Unigene #)/Unigene#. c5188 ESTs from the male Asparagus library were sequenced in collaboration with Mike Havey (University of Wisconsin) and Chris Town (TIGR). dMale and female Cucumis flower bud libraries described by Perl-Treves et al [71] eVaccinium young inflorescence library was provided by Jeannine Rowland (USDA) [72]. | ||||||
Albert et al. BMC Plant Biology 2005 5:5 doi:10.1186/1471-2229-5-5 |
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